Heat shock proteins: Possible biomarkers in pulmonary and extrapulmonary tuberculosis

Shekhawat, Seema D.; Jain, Ruchika; Gaherwar, Hari M.; Purohit, Hemant J.; Taori, Girdhar M.; Daginawala, Hatim F.; Kashyap, Rajpal S.

doi:10.1016/j.humimm.2013.11.007

Cited by 16 publications

(8 citation statements)

References 28 publications

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“…Our results also showed similar trends with the finding of a previous study that indicated that the serum APOC2 expression level was downregulated. Heat shock proteins might be a potential biomarker in pulmonary and extrapulmonary tuberculosis and may be useful for diagnosis and for understanding the pathogenesis of pulmonary and extrapulmonary TB [23]. HSP90 was also identified in our study.…”

Section: Discussionsupporting

confidence: 61%

Discovery and verification of serum differential expression proteins for pulmonary tuberculosis

et al. 2015

Tuberculosis

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Section: Discussionsupporting

confidence: 61%

Discovery and verification of serum differential expression proteins for pulmonary tuberculosis

et al. 2015

Tuberculosis

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“…MTB heat shock proteins (Hsps) 16, 65, 71 were detected using in house developed ELISA method as described elsewhere ( 13 ). Total IgG was estimated using ELISA protocol as described earlier by Husain et al ( 2 ).…”

Section: Methodsmentioning

confidence: 99%

Investigation of Immune Biomarkers Using Subcutaneous Model of M. tuberculosis Infection in BALB/c Mice: A Preliminary Report

et al. 2015

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Evaluation and screening of vaccines against tuberculosis depends on development of proper cost effective disease models along with identification of different immune markers that can be used as surrogate endpoints of protection in preclinical and clinical studies. The objective of the present study was therefore evaluation of subcutaneous model of M.tuberculosis infection along with investigation of different immune biomarkers of tuberculosis infection in BALB/c mice. Groups of mice were infected subcutaneously with two different doses : high (2×106 CFU) and low doses (2×102 CFU) of M.tuberculosis and immune markers including humoral and cellular markers were evaluated 30 days post M.tuberculosis infections. Based on results, we found that high dose of subcutaneous infection produced chronic disease with significant (p<0.001) production of immune markers of infection like IFNγ, heat shock antigens (65, 71) and antibody titres against panel of M.tuberculosis antigens (ESAT-6, CFP-10, Ag85B, 45kDa, GroES, Hsp-16) all of which correlated with high bacterial burden in lungs and spleen. To conclude high dose of subcutaneous infection produces chronic TB infection in mice and can be used as convenient alternative to aerosol models in resource limited settings. Moreover assessment of immune markers namely mycobacterial antigens and antibodies can provide us valuable insights on modulation of immune response post infection. However further investigations along with optimization of study protocols are needed to justify the outcome of present study and establish such markers as surrogate endpoints of vaccine protection in preclinical and clinical studies in future.

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“…We previously reported Hsp(s) as promising biomarkers in TB disease [36]. In this preliminary study, our results suggest that the evaluated Hsp(s) have the potential to distinguish individuals with active TB and LTBI, thus providing a new frontier in TB research.…”

Section: Parameters [Mean With Sd] Tb (N = 10)mentioning

confidence: 71%

Evaluation of heat shock proteins for discriminating between latent tuberculosis infection and active tuberculosis: A preliminary report

Shekhawat

Purohit

Taori

et al. 2016

Journal of Infection and Public Health

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The diagnosis of a latent tuberculosis infection (LTBI) is of the utmost concern. The available tests, the tuberculin skin test (TST) and the Quantiferon-TB Gold test (QFT-G) cannot discriminate between active TB and LTBI. Therefore, the aim of the study is to identify new biomarkers that can discriminate between active TB and LTBI and can also assess the risk of the individual developing active TB. In total, 55 blood samples were collected, of which 10 samples were from the active TB infection group, 10 were from the high-risk exposure group, 23 were from the low-risk exposure group, and 12 were from healthy controls living in a non-TB endemic area. A panel of heat shock proteins (Hsps), including host Hsp25, Hsp60, Hsp70, and Hsp90 and Mycobacterium tuberculosis (MTB) Hsp16, were evaluated in all of the collected samples using ELISA. The levels of the host Hsp(s) (Hsp25, Hsp60, Hsp70 and Hsp90) and MTB Hsp16 were significantly (p<0.05) elevated in the active TB group compared to the high-risk exposure group, the low-risk exposure group and the control group. Notably, the levels of the same panel of Hsp(s) were elevated in the high-risk exposure group compared to the low-risk exposure group. On follow-up, out of the 10 high-risk exposure participants, 3 converted into active TB, indicating that this group has the highest risk of developing TB. Thus, the evaluated panel of Hsp(s) can discriminate between LTBI and active TB. They can also identify individuals who are at the highest risk of developing active TB. Because they can be rapidly detected, Hsp(s) have an edge over the existing diagnostic tools for LTBI. The evaluation of these proteins will be useful in designing better diagnostic methods for LTBI.

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Heat shock proteins: Possible biomarkers in pulmonary and extrapulmonary tuberculosis

Cited by 16 publications

References 28 publications

Discovery and verification of serum differential expression proteins for pulmonary tuberculosis

Discovery and verification of serum differential expression proteins for pulmonary tuberculosis

Investigation of Immune Biomarkers Using Subcutaneous Model of M. tuberculosis Infection in BALB/c Mice: A Preliminary Report

Evaluation of heat shock proteins for discriminating between latent tuberculosis infection and active tuberculosis: A preliminary report

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