Evaluation of MALDI-TOF mass spectrometry for the identification of bacteria growing as biofilms

Gaudreau, Annie; Labrie, Josée; Goetz, Coralie; Dufour, Simon; Jacques, Mario

doi:10.1016/j.mimet.2018.01.003

Cited by 28 publications

(21 citation statements)

References 13 publications

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“…With this technology it is possible to obtain a protein profile, both from intact cells or cell extracts, and by comparing it to a database of bacterial reference mass spectra one can obtain a rapid identification of genus, species, and in some cases the sub species level [19]. In addition, recently, it has been increasingly used for the analysis of bacteria grown in biofilm [20,21]. Biofilm can be defined as a microbially-derived sessile community where cells are attached to each other and to a substratum embedded in a matrix of extracellular polymeric substance, and exhibit an altered phenotype regarding: growth, gene expression, and protein production [22].…”

Section: Discussionmentioning

confidence: 99%

Use of MALDI-TOF MS to Discriminate between Biofilm-Producer and Non-Producer Strains of Staphylococcus epidermidis

Caputo

Martino

Perfetto

et al. 2018

IJERPH

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For the management of Staphylococci coagulase-negative infection, often related to biofilm formation, rapid and accurate identification is necessary in choosing a correct antibiotic therapy. Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) is becoming increasingly important for bacterial identification over traditional methods. Our aim was to validate the use of MALDI to discriminate Staphylococcus epidermidis biofilm-producing strains. Clinical strains coming from suture wires were identified and their protein profiles were compared to that obtained from two ATCC reference strains (biofilm producer and non-producer). MALDI identified the eighteen isolates as S. epidermidis, combining sixteen profiles with the biofilm producer and two with the non-producer, confirming the results of crystal violet assay. Our data highlight that MALDI can be considered a good tool to discriminate between biofilm-producer and non-producer strains of S. epidermidis, thus helping to establish an effective antibiotic therapy.

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Section: Discussionmentioning

confidence: 99%

Use of MALDI-TOF MS to Discriminate between Biofilm-Producer and Non-Producer Strains of Staphylococcus epidermidis

Caputo

Martino

Perfetto

et al. 2018

IJERPH

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“…Authors showed that Maldi-Tof MS is applicable for routine identification and validation of foodborne yeasts. Gaudreau et al (2018) evaluated Maldi-Tof MS to identify bacteria from biofilms. They compared three sample preparation procedures on biofilms grown in vitro.…”

Section: Resultsmentioning

confidence: 99%

“…An optical technique with potential use to rapidly identify the microorganisms is the Maldi-Tof MS (Matrix Assisted Laser Desorption/Ionisation -Time of Flight Mass Spectrometry) (Microflex-Bruker Daltonics/ BioTyper™) (Chang et al, 2016;Pasternak et al, 2012;Gaudreau et al, 2018;Mayoral et al, 2018). In this technique, the sample is uniformly mixed in a large quantity of matrix.…”

Section: Introductionmentioning

confidence: 99%

Use of Maldi-Tof MS biosensor in microbial assessment of Brazilian kefir grains

et al. 2019

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RESUMOThe aim of this study was to evaluate the use of Maldi-Tof MS biosensor in microbial assessment of Brazilian kefir grains. Maldi-Tof MS is a new methodology for the rapid diagnosis of microorganisms. A total of 358 microorganisms were isolated, 31 were yeasts and 327 were bacteria (divided into lactic and acetic bacteria). Microbial colonies were grown in Luria-Bertani agar medium and incubated at 35 °C for 18h and used in the identification of species by Maldi-Tof MS. The microbial population identified in Brazilian kefir grains was Lactobacillus paracasei, Saccharomyces cerevisiae, Lactobacillus plantarum, Acetobacter pasteurianus, and Acetobacter syzygii. This study demonstrated a rapid and accurate identification of the Brazilian kefir grains microorganisms using the Maldi-Tof MS biosensor. In conclusion, the Maldi-Tof MS technology can facilitate the microbiological control in a fermentation process using kefir grains as starter cultures.O objetivo deste estudo foi avaliar o uso do biossensor de Maldi-Tof MS na avaliação microbiana de grãos de kefir. Maldi-Tof MS é uma nova metodologia para o diagnóstico rápido de micro-organismos. Um total de 358 microorganismos foram isolados, 31 foram leveduras e 327 foram bactérias (divididas em bactérias láticas e acéticas). Colônias microbianas foram cultivadas em meio Luria-Bertani (LB) e incubadas a 35 °C por 18h e posteriormente utilizadas na identificação a nível de espécies pelo Maldi-Tof MS. A população microbiana identificada nos grãos de kefir brasileiros foi Lactobacillus paracasei, Saccharomyces cerevisiae, Lactobacillus plantarum, Acetobacter pasteurianus e Acetobacter syzygii. Este estudo demonstrou uma identificação rápida e precisa dos micro-organismos de grãos de kefir brasileiros utilizando o biossensor Maldi-Tof MS. Em conclusão, a tecnologia Maldi-Tof MS pode facilitar o controle microbiológico em um processo de fermentação usando grãos de kefir como culturas iniciadoras. Palavras-chave: micro-organismos; bactérias; levedura; sensor biológico.This is an open access article under the CC BY licence Creative Commons 73 Use of Maldi-Tof MS biosensor in microbial assessment of Brazilian kefir grains 1

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“…Also, the method allows for differentiation between planktonic and biofilm cells, as these two populations display different spectra. The method has been tested in different species of Gram-positive and negative bacteria [ 84 ].…”

Section: Diagnosis Of Biofilm Mediated Infectionsmentioning

confidence: 99%

Targeting the Bacterial Protective Armour; Challenges and Novel Strategies in the Treatment of Microbial Biofilm

et al. 2018

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Infectious disease caused by pathogenic bacteria continues to be the primary challenge to humanity. Antimicrobial resistance and microbial biofilm formation in part, lead to treatment failures. The formation of biofilms by nosocomial pathogens such as Staphylococcus aureus (S. aureus), Pseudomonas aeruginosa (P. aeruginosa), and Klebsiella pneumoniae (K. pneumoniae) on medical devices and on the surfaces of infected sites bring additional hurdles to existing therapies. In this review, we discuss the challenges encountered by conventional treatment strategies in the clinic. We also provide updates on current on-going research related to the development of novel anti-biofilm technologies. We intend for this review to provide understanding to readers on the current problem in health-care settings and propose new ideas for new intervention strategies to reduce the burden related to microbial infections.

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Evaluation of MALDI-TOF mass spectrometry for the identification of bacteria growing as biofilms

Cited by 28 publications

References 13 publications

Use of MALDI-TOF MS to Discriminate between Biofilm-Producer and Non-Producer Strains of Staphylococcus epidermidis

Use of MALDI-TOF MS to Discriminate between Biofilm-Producer and Non-Producer Strains of Staphylococcus epidermidis

Use of Maldi-Tof MS biosensor in microbial assessment of Brazilian kefir grains

Targeting the Bacterial Protective Armour; Challenges and Novel Strategies in the Treatment of Microbial Biofilm

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