Use of MALDI-TOF MS to Discriminate between Biofilm-Producer and Non-Producer Strains of Staphylococcus epidermidis

Caputo, Pina; Martino, Maria; Perfetto, Brunella; Iovino, Francesco; Donnarumma, Giovanna

doi:10.3390/ijerph15081695

Cited by 19 publications

(13 citation statements)

References 29 publications

(36 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been used as a routine technique for fungal identification at species level, and represents an important phenotypic methodology used for fungal identification [23][24][25]. In addition, MALDI-TOF MS has been used to identify both yeasts and filamentous fungal species and other microbial taxonomic groups [26,27].…”

Section: Introductionmentioning

confidence: 99%

First Comprehensive Report of Clinical Fusarium Strains Isolated in the State of Sao Paulo (Brazil) and Identified by MALDI-TOF MS and Molecular Biology

et al. 2019

View full text Add to dashboard Cite

The aim of this study was to compare the performance of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), phenotypic and molecular methods for the identification of Fusarium species complexes isolated from clinical cases in the State of Sao Paulo (Brazil) between the years 2001 and 2017. Sequencing of ITS region of ribosomal DNA and elongation factor 1 alpha gene (ET1α) were used as reference method in the analysis of a total of 108 Fusarium spp. clinical strains isolated from human hosts with superficial and systemic infections. Agreement between MALDI-TOF-MS and molecular data was observed for 97 out of 108 clinical isolates (89.8%), whereas five (4.6%) and six (5.5%) clinical isolates were misidentified and were not identified by MALDI-TOF MS, respectively. ITS region sequences and MALDI-TOF MS mass spectra identified and grouped correctly most of Fusarium clinical isolates at species complex level. This investigation highlights the potential of MALDI-TOF MS technique as a fast and cost-efficient alternative for clinical Fusarium identification. However, MALDI-TOF MS requires a more accurate and larger database. This work is the first comprehensive report for Fusarium population, based on phenotypic analyses, proteomic profile by MALDI-TOF and phylogenetic analyses of Fusarium species complexes isolated from clinical cases in the State of Sao Paulo, Brazil.

show abstract

Section: Introductionmentioning

confidence: 99%

First Comprehensive Report of Clinical Fusarium Strains Isolated in the State of Sao Paulo (Brazil) and Identified by MALDI-TOF MS and Molecular Biology

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Microbial identification is performed in 60 s and has been used in the diagnosis of several infectious diseases around the world (Singhal et al 2015). Currently, this methodology is the target of researchers and applied biofilm studies, since MALDI-TOF allows the differentiation of planktonic cells and biofilms due to profiles of different mass spectra (Caputo et al 2018). The method was tested on different species of Gram-positive and negative bacteria and fungi (Kubesova et al 2012;Pereira et al 2015;Caputo et al 2018), showing that it is possible, through MALDI-TOF, to differentiate microorganisms that form weak or strong biofilms, based on their molecular mass size.…”

Section: Detection Of Biofilms In Vivomentioning

confidence: 99%

Diagnosis of biofilm infections: current methods used, challenges and perspectives for the future

Silva

Marques

Röder³

2021

J Appl Microbiol

View full text Add to dashboard Cite

The diagnosis of biofilms continues to be a challenge, and there is no standardized protocol for such a diagnosis in clinical practice. In addition, some proposed methodologies are expensive to require significant amounts of time and a high number of trained staff, making them impracticable for clinical practice. In recent years, mass spectrophotometry/MALDI-TOF has been applied it in biofilm studies. However, due to several problems and limitations of the technique, MALDI-TOF is far from being the gold standard for identifying biofilm formation. The omics analysis may prove to be a promising strategy for the diagnosis of biofilms in clinical laboratories since it allows the identification of pathogens in less time than needed for conventional techniques and in a more specific manner. However, omic tools are expensive and require qualified technical expertise and an analysis of the data obtained needs to be careful not to neglect sub-populations in the biofilm. More studies must therefore be developed for creating a protocol that guarantees rapid biofilm identification, ensuring greater chances of success in infection control. This review discusses the current methods of microbial biofilm detection and future perspectives for its diagnosis in clinical practice.

show abstract

“…MALDI-TOF MS can efficiently identify bacteria directly in samples prepared from biofilms [8] and discriminate between biofilm-producer and non-producer strains, using the same method and software that is used in the identification stage [25]. Among the three potential biomarker peaks of biofilm production we detected, the 6074 − /6102 + phenotype was associated with the rmlA + genotype, which could be used as a potential biomarker peak of strong https://doi.org/10.1371/journal.pone.0244751.g002…”

Section: Plos Onementioning

confidence: 99%

Discrimination of biofilm-producing Stenotrophomonas maltophilia clinical strains by matrix-assisted laser desorption ionization–time of flight

et al. 2020

View full text Add to dashboard Cite

Stenotrophomonas maltophilia is a Gram-negative drug-resistant pathogen responsible for healthcare-associated infections. The aim was to search for biomarker peaks that could rapidly detect biofilm production in S. maltophilia clinical isolates obtained from two tertiary care hospitals in Mexico. Isolates were screened for the presence of biofilm-associated genes, in which the fsnR gene was associated with biofilm production (p = 0.047), whereas the rmlA+ genotype was associated with the rpfF- genotype (p = 0.017). Matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) mass spectra comparison yielded three potential biomarker peaks (4661, 6074, and 6102 m/z) of biofilm-producing rmlA+ and rpfF- genotypes with >90% sensitivity (p<0.001). MALDI-TOF MS analyses showed a correlation between the relative abundance of 50S ribosomal proteins (L30 and L33) and the presence of the fnsR, rmlA and rpfF-2 genes, suggested to play a role in biofilm formation. Isolates obtained in the intensive care unit showed low clonality, suggesting no transmission within the hospital ward. The detection of biomarkers peaks by MALDI-TOF MS could potentially be used to early recognize and discriminate biofilm-producing S. maltophilia strains and aid in establishing appropriate antibiotic therapy.

show abstract

Use of MALDI-TOF MS to Discriminate between Biofilm-Producer and Non-Producer Strains of Staphylococcus epidermidis

Cited by 19 publications

References 29 publications

First Comprehensive Report of Clinical Fusarium Strains Isolated in the State of Sao Paulo (Brazil) and Identified by MALDI-TOF MS and Molecular Biology

First Comprehensive Report of Clinical Fusarium Strains Isolated in the State of Sao Paulo (Brazil) and Identified by MALDI-TOF MS and Molecular Biology

Diagnosis of biofilm infections: current methods used, challenges and perspectives for the future

Discrimination of biofilm-producing Stenotrophomonas maltophilia clinical strains by matrix-assisted laser desorption ionization–time of flight

Contact Info

Product

Resources

About