First Comprehensive Report of Clinical Fusarium Strains Isolated in the State of Sao Paulo (Brazil) and Identified by MALDI-TOF MS and Molecular Biology

Paziani, Mário Henrique; Carvalho, Ludmilla Tonani; Melhem, Márcia de Souza Carvalho; Almeida, Margarete Teresa Gottardo de; Silva, Maria Emília Nadaletto Bonifácio da; Martínez, Roberto; Santos, Cledir; Kress, Márcia Regina von Zeska

doi:10.3390/microorganisms8010066

Cited by 9 publications

(13 citation statements)

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“…Thus, the Fusarium species was only reported when determined by genomic sequencing or MALDI-TOF mass spectrometry, because these techniques have high agreement rates (89.8%-97.0%) (31)(32)(33). All remaining IF cases in our series were reported as Fusarium spp.…”

Section: Discussionmentioning

confidence: 95%

“…Although all IF cases in this retrospective study met standard criteria ( 16 , 17 ), we could not determine the causative species in every case. Thus, the Fusarium species was only reported when determined by genomic sequencing or MALDI-TOF mass spectrometry, because these techniques have high agreement rates (89.8%–97.0%) ( 31 – 33 ). All remaining IF cases in our series were reported as Fusarium spp.…”

Section: Discussionmentioning

confidence: 99%

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Invasive Fusariosis in Nonneutropenic Patients, Spain, 2000–2015

Pérez‐Nadales¹,

Alastruey‐Izquierdo²,

Linares-Sicilia³

et al. 2021

Emerg. Infect. Dis.

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Invasive Fusariosis in Nonneutropenic Patients, Spain, 2000–2015

Pérez‐Nadales¹,

Alastruey‐Izquierdo²,

Linares-Sicilia³

et al. 2021

Emerg. Infect. Dis.

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“…2015 , Sleiman et al. 2016 , Paziani et al. 2020 ) and clinically relevant Bisifusarium ( Triest et al.…”

Section: Maldi-tofmentioning

confidence: 99%

“…2020 ) and clinically relevant Bisifusarium ( Triest et al. 2015 , Paziani et al. 2020 ) and Neocosmospora species ( Marinach-Patrice et al.…”

Section: Maldi-tofmentioning

confidence: 99%

Fusarium: more than a node or a foot-shaped basal cell

Crous¹,

Lombard²,

Sandoval-Denis³

et al. 2021

Studies in Mycology

182

133

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Recent publications have argued that there are potentially serious consequences for researchers in recognising distinct genera in the terminal fusarioid clade of the family Nectriaceae . Thus, an alternate hypothesis, namely a very broad concept of the genus Fusarium was proposed. In doing so, however, a significant body of data that supports distinct genera in Nectriaceae based on morphology, biology, and phylogeny is disregarded. A DNA phylogeny based on 19 orthologous protein-coding genes was presented to support a very broad concept of Fusarium at the F1 node in Nectriaceae . Here, we demonstrate that re-analyses of this dataset show that all 19 genes support the F3 node that represents Fusarium sensu stricto as defined by F. sambucinum (sexual morph synonym Gibberella pulicaris ). The backbone of the phylogeny is resolved by the concatenated alignment, but only six of the 19 genes fully support the F1 node, representing the broad circumscription of Fusarium. Furthermore, a re-analysis of the concatenated dataset revealed alternate topologies in different phylogenetic algorithms, highlighting the deep divergence and unresolved placement of various Nectriaceae lineages proposed as members of Fusarium . Species of Fusarium s. str. are characterised by Gibberella sexual morphs, asexual morphs with thin- or thick-walled macroconidia that have variously shaped apical and basal cells, and trichothecene mycotoxin production, which separates them from other fusarioid genera. Here we show that the Wollenweber concept of Fusarium presently accounts for 20 segregate genera with clear-cut synapomorphic traits, and that fusarioid macroconidia represent a character that has been gained or lost multiple times throughout Nectriaceae . Thus, the very broad circumscription of Fusarium is blurry and without apparent synapomorphies, and does not include all genera with fusarium-like macroconidia, which are spread throughout Nectriaceae ( e.g. , Cosmosporella , Macroconia , Microcera ). In this study four new genera are introduced, along with 18 new species and 16 new combinations. These names convey information about relationships, morphology, and ecological preference that would otherwise be lost in a broader definition of Fusarium . To assist users to correctly identify fusarioid genera and species, we introduce a new online identification database, Fusarioid-ID, accessible at www.fusarium.org . The database comprises partial sequences from multiple genes commonly used to identify fusarioid taxa ( ...

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“…Calibration of the MALDI-TOF equipment was performed using freshly in situ extracted ribosomal proteins of E. coli strain DH5-alpha, as previously described by Matos et al 30 and Paziani et al 31 Cells of E. coli DH5-alpha were grown on nutrient agar medium (Merck) at 37°C for 20 h. Briefly, fresh E. coli DH5-alpha biomass was transferred to MALDI sample plate and MALDI matrix solution was added and mixed. To evaluate reproducibility, each sample was applied in triplicate.…”

Section: Maldi-tof Ms Analyses Of Proteinsmentioning

confidence: 99%

Influence of S. cerevisiae and P. kluyveri as starters on chocolate flavour

et al. 2021

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BACKGROUND: Fermented cocoa beans (Theobroma cacao L.) are a pivotal raw material for chocolate production. A cocktail yeast applied in the cocoa fermentation process can promote the formation of pleasant metabolites. Saccharomyces, Pichia and Hanseniaspora have been widely used in fermentation to improve the final product organoleptic profile, highlighting that fermentation is a critical point for chocolate flavour precursor production. This study aims to evaluate the impact of Pichia kluyveri and Saccharomyces cerevisiae strains as starter cultures on the fermentation for two cocoa hybrids, FA13 and CEPEC2002. RESULTS: During fermentation processes, volatile organic compounds (VOCs) and protein profiles were assessed. Chocolates produced were also assessed regarding the presence of VOCs. Eighty VOCs were identified using gas chromatography coupled to mass spectrometry analysis. Mass spectrometry provided the protein profile evolution during fermentation and showed that the profiles changed with inoculation type (spontaneous versus inoculated fermentation). Chocolate obtained from FA13 inoculated with S. cerevisiae strain contained a greater amount of organics acids, being categorised as sourer than chocolate produced by spontaneous fermentation of FA13. CEPEC2002 inoculated with S. cerevisiae strain in co-culture with P. kluyveri strain generated less sour and sweeter chocolate than spontaneous fermentation only.CONCLUSIONS: Chocolates from inoculated assays with starter cultures were more accepted by evaluators, highlighting that P. kluyveri and S. cerevisiae influence the composition of VOCs. Besides, protein profiles also changed throughout fermentation. Further investigation should be conducted to clarify protein degradation dynamics during inoculated fermentations to define which of the microbial cultures positively affect the chocolate sensory characteristics.

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First Comprehensive Report of Clinical Fusarium Strains Isolated in the State of Sao Paulo (Brazil) and Identified by MALDI-TOF MS and Molecular Biology

Cited by 9 publications

References 50 publications

Invasive Fusariosis in Nonneutropenic Patients, Spain, 2000–2015

Invasive Fusariosis in Nonneutropenic Patients, Spain, 2000–2015

Fusarium: more than a node or a foot-shaped basal cell

Influence of S. cerevisiae and P. kluyveri as starters on chocolate flavour

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