Evaluation of invitro α-amylase and α-glucosidase inhibitory potential of N 2 O 2 schiff base Zn complex

Balan, Kannan; Ratha, Periyasamy; Prakash, Govindan; Viswanathamurthi, Periasamy; Palvannan, Thayumanavan

doi:10.1016/j.arabjc.2014.07.002

Cited by 37 publications

(25 citation statements)

References 29 publications

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“…One therapeutic approach is the prevention of carbohydrate absorption after food intake, which is facilitated by inhibition of enzymes including α-amylase. In the human species, α-amylase is present in both salivary and pancreatic secretions [4]. This enzyme hydrolyzes complex polysaccharides to produce oligosaccharides and disaccharides which are absorbed through the small intestines into the hepatic portal vein and increase postprandial glucose levels [40,41].…”

Section: Discussionmentioning

confidence: 99%

“…The management of diabetes in the absence of any side effects is up to this time a challenge to the medical system [3]. The therapeutic approaches for reducing postprandial hyperglycemia is to hinder the glucose absorption by the inhibition of carbohydrate-hydrolyzing enzymes, such as α-amylase [4]. Thus, the slowness of the α-amylase action by inhibitors could be one of the most efficient approaches to control Type 2 DM.…”

Section: Introductionmentioning

confidence: 99%

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Exploring Antioxidant and Enzymes (A-Amylase and B-Glucosidase) Inhibitory Activity of Morinda lucida and Momordica charantia Leaves from Benin

Chokki

Cudalbeanu

Zongo

et al. 2020

Foods

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Background: Momordica charantia Linn. (Cucurbitaceae), the wild variety of bitter melon and Morinda lucida Benth (Rubiaceae) were commonly used as a popular folk medicine in Benin. This research focused to measure the antioxidant and enzyme inhibitory effects of M. charantia and M. lucida leaves and their antidiabetic activity. Methods: Antioxidant activities were evaluated by micro-dilution technique using DPPH free radical scavenging activity and β-carotene-linoleate bleaching assay. The α-amylase inhibition assay was carried out utilizing the 3,5-dinitrosalicylic acid procedure, while β-glucosidase inhibition assay was demonstrated using as substrate p-nitrophenyl-β-D-glucopyranoside (PNPG). HPLC-DAD analysis was realized using a high-performance liquid chromatography systems with diode-array detector, L-3000. Results: Chlorogenic acid, epicatechin, daidzein, rutin, naringin, quercetin, naringenin and genistein were identified as polyphenol compounds in the both plants extract. Dichloromethane and ethyl acetate extracts showed a good α-amylase inhibitory activity (56.46 ± 1.96% and 58.76 ± 2.74% respectively). M. lucida methanolic extract has shown IC50 of 0.51 ± 0.01 mg/mL, which is the lowest for DPPH scavenging activity. M. lucida dichloromethane extract showed the highest inhibitory capacity of β-glucosidase activity (82.11. ± 2.15%). Conclusion: These results justify some traditional medicinal uses of both plants. The purified fractions could be used in future formulations, possibly incorporated in functional foods to combat certain diseases.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Exploring Antioxidant and Enzymes (A-Amylase and B-Glucosidase) Inhibitory Activity of Morinda lucida and Momordica charantia Leaves from Benin

Chokki

Cudalbeanu

Zongo

et al. 2020

Foods

View full text Add to dashboard Cite

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“…The reaction was stopped with 50 μL of 96 mM 3,5,dinitrosalicylic acid color reagent. The microplate was then incubated in a boiling water bath for 5 min and cooled to room temperature . Absorbance was measured at 540 nm using a microplate reader.…”

Section: Methodsmentioning

confidence: 99%

Synthesis and Biological Evaluation of some 3d Metal Complexes with a Novel Heterocyclic Schiff Base

Thomas

Mohanan

2017

J Chinese Chemical Soc

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A heterocyclic Schiff base was prepared by condensing 3‐acetylcoumarin with 2‐amino‐3‐carboxyethyl‐4,5,6,7‐tetrahydrobenzo[b]thiophene. Such Schiff bases derived from two different heterocyclic moieties are rare and expected to have properties surpassing those of either of the parent compounds in effectiveness of complex formation and biological activities. This ligand formed a series of complexes with manganese(II), cobalt(II), nickel(II), copper(II), and zinc(II) ions. The ligand and the metal complexes were characterized by various physicochemical and spectral studies. These included elemental analysis, molar conductance, magnetic susceptibility, as well as UV–vis, IR, 1H NMR, 13C NMR, and ESR spectral studies. The ESR spectral data adequately supported the covalent nature of the metal–ligand bonds. The ligand possessed a hexagonal crystal structure, but on complexation the crystallinity was lost. The fluorescence spectra of the ligand and its metal complexes in DMSO were also recorded. The ligand and the metal complexes were screened for their antimicrobial activities, and it was observed that the metal complexes are more active than the ligand. The α‐amylase inhibitory activity and the DNA cleavage activity of the ligand and the metal complexes were also examined. in vitro antitumor activity of the copper(II) complex was assayed against human cervical carcinoma cells (HeLa cell line), showing that the complex exhibited promising antitumor activity on the HeLa cell line.

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“…N-Mannich products were prepared by a one-pot, three-component condensation reaction, performed under reflux conditions. Formaldehyde (S), amine (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13), and tetrahydroquinoline (F) were placed in a pressure tube and dissolved in ethanol and a catalytic amount of HCl was added. The mixture was refluxed at 80 • C for~5-7 h, leading to the formation of the N-Mannich base.…”

Section: Chemistrymentioning

confidence: 99%

“…α-Amylase is a key enzyme responsible for the hydrolysis of carbohydrates. Inhibiting α-amylase is a successful approach to controlling postprandial blood sugar within the acceptable range, which is a key factor in controlling diabetes mellitus (DM) [8][9][10].…”

Section: Introductionmentioning

confidence: 99%

One-Pot Multicomponent Synthesis and Bioevaluation of Tetrahydroquinoline Derivatives as Potential Antioxidants, α-Amylase Enzyme Inhibitors, Anti-Cancerous and Anti-Inflammatory Agents

et al. 2020

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Mankind has always suffered from multiple diseases. Therefore, there has been a rigorous need in the field of medicinal chemistry for the design and discovery of new and potent molecular entities. In this work, thirteen tetrahydroquinoline derivatives were synthesized and evaluated biologically for their antioxidant, α-amylase enzyme inhibitory, anti-proliferative and anti-inflammatory activities. SF8 showed the lowest IC50 of 29.19 ± 0.25 µg/mL by scavenging DPPH free radicals. SF5 showed significant antioxidant activity in total antioxidant capacity (TAC) and total reducing power (TRP) assays. SF5 and SF9 showed the maximum inhibition of α-amylase enzyme i.e., 97.47% and 89.93%, respectively, at 200 µg/mL concentration. Five compounds were shortlisted to determine their anti-proliferative potential against Hep-2C cells. The study was conducted for 24, 48 and 72 h. SF8 showed significant results, having an IC50 value of 11.9 ± 1.04 µM at 72 h when compared with standard cisplatin (IC50 value of 14.6 ± 1.01 µM). An in vitro nitric oxide (NO) assay was performed to select compounds for in vivo anti-inflammatory activity evaluation. SF13 scavenged the NO level to a maximum of 85% at 50 µM concentration, followed by SF1 and SF2. Based on the NO scavenging assay results, in vivo anti-inflammatory studies were also performed and the results showed significant activity compared to the standard, acetylsalicylic acid (ASA).

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Evaluation of invitro α-amylase and α-glucosidase inhibitory potential of N 2 O 2 schiff base Zn complex

Cited by 37 publications

References 29 publications

Exploring Antioxidant and Enzymes (A-Amylase and B-Glucosidase) Inhibitory Activity of Morinda lucida and Momordica charantia Leaves from Benin

Exploring Antioxidant and Enzymes (A-Amylase and B-Glucosidase) Inhibitory Activity of Morinda lucida and Momordica charantia Leaves from Benin

Synthesis and Biological Evaluation of some 3d Metal Complexes with a Novel Heterocyclic Schiff Base

One-Pot Multicomponent Synthesis and Bioevaluation of Tetrahydroquinoline Derivatives as Potential Antioxidants, α-Amylase Enzyme Inhibitors, Anti-Cancerous and Anti-Inflammatory Agents

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