Evaluation of inoculum size and fermentation period for bacterial cellulose production from sago liquid waste

Yanti, Nur Arfa; Ahmad, Sitti Wirdhana; Muhiddin, Nurhayani H.

doi:10.1088/1742-6596/1116/5/052076

Cited by 14 publications

(10 citation statements)

References 12 publications

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“…Low concentrations below 30% the degradation power is not very good and above 40% the degradability tends to be constant. The addition of inoculum concentration in the fermentation medium resulted in competition among microorganisms in using nutrients, so that the growth of microorganisms and production were disrupted [28]. Optimal inoculum concentration causes rapid fungal growth characterized by the formation of a dense mass of mycelium accompanied by a rapidly ripening conidiospore [19].…”

Section: Discussionmentioning

confidence: 99%

Optimization of the Degradation Power of Aspergillus flavus DFSP.J1 and Aspergillus niger DFSP. J4 in Degrading Sago Bioplastics

Gunaedi¹,

Mawardi²,

Rumbrawer³

2021

AJOB

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Bioplastics made from sago flour have the potential to be developed into plastic-based industrial materials that are environmentally friendly and easily degraded by microorganisms. A. flavus DSFP.J1 and A. niger DFSP.J4 are microorganisms from a type of fungus that are proven to be able to degrade sago bioplastics, but have not yet obtained optimal degradation power. Therefore, this study aimed to obtain optimal conditions of the two isolates of fungi in degrading bioplastics made from sago flour at variable inoculum concentrations of 10, 20, 30,40, 50% v/w. The manufacture of sago bioplastics is done by adding acetic acid as a catalyst and added glycerol to form plastic properties in 15% sago flour, after being formed and cut to a size of 1 cm2. Observations to determine the degradation power were carried out after an incubation period of seven days to the next five weeks. The results obtained showed that the optimal conditions of A. flavus DFSP.J1 and in degrading sago bioplastics at 40% inoculum concentration with a degradation power of 56.52% in the fifth week of observation and tended to increase the degradation power on the following day, as well as A. niger DFSP.J4 optimally degrades at 30% inoculum concentration with a degradation power of 55.90% in the fifth week of observation. The results of the research, it can be concluded. Both the fungus A. flavus DFSP. J1 and A. niger DFSP.J4 showed that potentialy to be developed for further research to increase its degradation power higher and faster.

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Section: Discussionmentioning

confidence: 99%

Optimization of the Degradation Power of Aspergillus flavus DFSP.J1 and Aspergillus niger DFSP. J4 in Degrading Sago Bioplastics

Gunaedi¹,

Mawardi²,

Rumbrawer³

2021

AJOB

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“…The larger inoculum size with active seed culture minimizes the length of adaptation (lag period) phase and facilitates the biomass concentration with a short fermentation time leading to higher production of exopolysaccharides such as BC [48]. However, if the inoculum concentration is in excess, there would be competition between the cells in utilizing nutrients, which disrupts the bacterial growth and thereby reduces the production of BC [4,49,50]. The rapid consumption of nutrients contained in the medium at larger inoculum size reduces the efficiency of fermentation [48].…”

Section: Assessment Of Cell Ability To Produce Bcmentioning

confidence: 99%

Preparation of Komagataeibacter xylinus Inoculum for Bacterial Cellulose Biosynthesis Using Magnetically Assisted External-Loop Airlift Bioreactor

et al. 2021

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The aim of this study was to demonstrate the applicability of a novel magnetically assisted external-loop airlift bioreactor (EL-ALB), equipped with rotating magnetic field (RMF) generators for the preparation of Komagataeibacterxylinus inoculum during three-cycle repeated fed-batch cultures, further used for bacterial cellulose (BC) production. The fermentation carried out in the RMF-assisted EL-ALB allowed to obtain an inoculum of more than 200× higher cellular density compared to classical methods of inoculum preparation. The inoculum obtained in the RMF-assisted EL-ALB was characterized by a high and stable metabolic activity during repeated batch fermentation process. The application of the RMF-assisted EL-ALB for K. xylinus inoculum production did not induce the formation of cellulose-deficient mutants. It was also confirmed that the ability of K. xylinus to produce BC was at the same level (7.26 g/L of dry mass), regardless of inoculum age. Additionally, the BC obtained from the inoculum produced in the RMF-assisted EL-ALB was characterized by reproducible water-related properties, mechanical strength, nano-fibrillar structure and total crystallinity index. The lack of any negative impact of inoculum preparation method using RMF-assisted EL-ALB on BC properties is of paramount value for its future applications, including use as a biomaterial in tissue engineering, wound healing, and drug delivery, where especially BC liquid capacity, nanostructure, crystallinity, and mechanical properties play essential roles.

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“…The recent biodegradable plastics (BPs) development has been developed from bacterial biomass, which is becoming an interesting topic to be studied [ 3 , 4 , 5 ], mainly to be applied as scaffolding materials, bone tissue engineering, nerve regeneration, blood vessel replacement [ 4 ], food industry [ 4 , 5 ], and burn wound dressing [ 4 ]. BC is one of the promising biomaterials which can be developed as a food packaging plastic material [ 6 ] that is produced by acetic acid bacteria through the fermentation of high carbohydrate-containing substrates such as agricultural and industrial waste [ 7 , 8 , 9 ]. The BC production is mainly facilitated in the laboratory by non-pathogenic bacteria, such as Acetobacter xylinum [ 7 , 8 , 9 , 10 ] and Gluconacetobacter xylinum [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

“…BC is one of the promising biomaterials which can be developed as a food packaging plastic material [ 6 ] that is produced by acetic acid bacteria through the fermentation of high carbohydrate-containing substrates such as agricultural and industrial waste [ 7 , 8 , 9 ]. The BC production is mainly facilitated in the laboratory by non-pathogenic bacteria, such as Acetobacter xylinum [ 7 , 8 , 9 , 10 ] and Gluconacetobacter xylinum [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

“…Given this problem, the abundant waste of polysaccharides in Southeast Sulawesi, Indonesia, or hence the popular sago liquid waste by-products produced from the sago starch processing industry were disposed of in the surrounding environment. In order to mitigate the environmental issue due to the use of synthetic plastics, sago liquid waste was treated to produce BC [ 9 , 10 ]. The properties of BC from sago liquid waste were supportive to be applied as plastic [ 6 ] so that BC has the potential to be made into an edible film for food packaging.…”

Section: Introductionmentioning

confidence: 99%

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Properties and Application of Edible Modified Bacterial Cellulose Film Based Sago Liquid Waste as Food Packaging

et al. 2021

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Bacterial cellulose (BC) based on sago liquid waste has been developed to be used as food packaging. This study investigated the physicochemical and mechanical properties of modified BC film and its application as food packaging. The modified BC film performed carboxymethyl cellulose (CMC) as a stabilizer and glycerol as a plasticizer. Films were prepared by casting technique using BC as the primary material and composites with various concentrations of CMC and glycerol (0.5%, 1%, and 1.5%, v/v). BC film was applied as the packaging of meat sausage, and the quality of meat sausage was measured based on weight loss, moisture content, pH, protein content, and total microbial count. The addition of CMC and glycerol influences the physical and mechanical properties of BC composites film. The best mechanical properties of edible BC film were collected by adding 1% CMC and 1% glycerol with a tensile strength of 17.47 MPa, elongation at a break of 25.60%, and Young’s modulus of 6.54 GPa. FTIR analysis showed the characteristic bands of BC, and the addition of CMC and glycerol slightly changed the FTIR spectrum of the composites. The utilization of modified BC-based sago liquid waste film as the packaging of meat sausage could maintain sausage quality during 6 days of storage at room temperature. Therefore, edible BC film has the potential to be used as food packaging.

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Evaluation of inoculum size and fermentation period for bacterial cellulose production from sago liquid waste

Cited by 14 publications

References 12 publications

Optimization of the Degradation Power of Aspergillus flavus DFSP.J1 and Aspergillus niger DFSP. J4 in Degrading Sago Bioplastics

Optimization of the Degradation Power of Aspergillus flavus DFSP.J1 and Aspergillus niger DFSP. J4 in Degrading Sago Bioplastics

Preparation of Komagataeibacter xylinus Inoculum for Bacterial Cellulose Biosynthesis Using Magnetically Assisted External-Loop Airlift Bioreactor

Properties and Application of Edible Modified Bacterial Cellulose Film Based Sago Liquid Waste as Food Packaging

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