Evaluation of in vitro growth-inhibitory effect of carvacrol and thymol combination against Staphylococcus aureus in liquid and vapour phase using new broth volatilization chequerboard method

Netopilova, M; Houdková, Markéta; Rondevaldova, Johana; Kmet, V.; Kokoška, Ladislav

doi:10.1016/j.fitote.2018.07.002

Cited by 20 publications

(11 citation statements)

References 24 publications

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“…Aspergillus flavus O 100 % 0.33 µL/mL [81] Aspergillus niger O 100 % 0.33 µL/mL [81] Aspergillus ochraceus O 100 % 0.33 µL/mL [81] Continued ▶ Aspergillus fumigatus MIC 0.025 µg/mL [92] Aspergillus niger MIC 0.020 µg/mL; 3.08 µL/L air [91,93] Aspergillus nomius MIC 0.060 µg/mL [92] Bacillus subtilis MIC 0.250 µg/mL [92] Candida albicans MIC 0.012 µg/mL [92] Cryptococcus neoformans MIC 0.060 µg/mL [92] Escherichia. coli MIC 0.050 µg/mL [92] Eupenicillum hirayamae MIC 0.060 µg/mL [92] Mycobacterium smegmatis MIC 0.012 µg/mL [92] Penicillium cinnamopurpureum MIC 0.025 µg/mL [92] Penicillium expansum MIC 0.012 µg/mL [92] Penicillium viridicatum MIC 0.060 µg/mL [92] Pseudomonas aeruginosa MIC 0.012 µg/mL [92] Salmonella typhimurium MIC 0.025 µg/mL; IZ 57.5-85.0 mm for 10 µL of EO [91,94] Staphylococcus aureus MIC 0.012 µg/mL [92] Streptococcus pyogenes MIC 0.012 µg/mL [92] Trichophyton rubrum MIC 0.050 µg/mL [92] Continued ▶ Staphylococcus aureus MIC 64-1024 µg/mL [65,66] Streptococcus pneumoniae MIC 64 µg/mL [65] disc volatilization method…”

Section: Asteraceae Shoot Disc Volatilization Methodsmentioning

confidence: 99%

“…MIC 40 mg/L [123] Phomopsis spp. MIC 40 mg/L [123] Pseudomonas fluorescenc MID 10 mg/petri dish [122] Saccharomyces cerevisiae MID 5 mg/petri dish [122] Salmonella Aspergillus flavus MID 56 mg/L air [125] Aspergillus versicolor MID 28 mg/L air [125] Eurotium amstelodami MID 28 mg/L air [125] Eurotium chevalieri MID 14 mg/L air [125] Continued ▶ Aspergillus flavus MID 56 mg/L air [125] Aspergillus versicolor MID 56 mg/L air [125] Eurotium amstelodami MID 28 mg/L air [125] Eurotium chevalieri MID 56 mg/L air [125] Penicillium adametzii MID 28 mg/L air [125] Penicillium citrinum MID 28 mg/L air [125] Penicillium griseofulvum MID 56 mg/L air [125] Penicillium islandicum MID 28 mg/L air [125] disc volatilization method Bacillus cereus IZ 35 mm [101] Candida albicans MIC 0.0075 µg/mL [110] Candida glabrata MIC 0.0075-0.0300 µg/mL [110] Candida tropicalis MIC 0.0015-0.0300 µg/mL [110] Enterococcus faecalis MID 100-1600 mg/L [124] Continued ▶ Staphylococcus aureus MIC 32-1024 µg/mL [65,66] Streptococcus pneumoniae MIC 32 µg/mL [65] disc volatilization method…”

Section: Asteraceae Shoot Disc Volatilization Methodsmentioning

confidence: 99%

“…Netopilova et al [66] modified the broth microdilution volatilization method for the evaluation of the combinatory effects of volatiles using a chequerboard design and allowing the determination of FIC indices. It differs from the method described above only in the layout of the assay plate (▶ Fig.…”

Section: Broth Microdilution Volatilization Methodsmentioning

confidence: 99%

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Volatile Antimicrobial Agents and In Vitro Methods for Evaluating Their Activity in the Vapour Phase: A Review

Houdková

Kokoška

2020

Planta Med

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This review summarizes data on the in vitro antimicrobial effectiveness of volatile agents of plant origin and in vitro methods for evaluating their activity in the vapour phase. As a result of literature analysis, the antimicrobial efficacy of vapours from 122 different plant species and 19 pure compounds examined in 61 studies using different in vitro tests against a broad spectrum of microorganisms was identified and summarized. In addition, 11 different techniques found in the literature are described in detail. An original classification of methods based on the solid and liquid matrix volatilization principle is proposed because carrier medium/matrix selection is crucial for the volatilization of any agents tested. This review should be useful for medicinal, pharmaceutical, food, and agricultural experts working in areas related to the management of infectious diseases (especially respiratory and skin infections), food preservation (active packaging), and protection of agriculture products (controlled atmosphere). It may also stimulate the interest of pharmaceutical, cosmetic, food, and agriculture industries in the research and development of new antimicrobial agents of natural origin. Since several original apparatuses previously developed for antimicrobial susceptibility testing in the vapour phase are described in this review, labware manufacturers may also be interested in this topic. The review also provides specific guidelines and recommendations for researchers studying the antimicrobial activity of volatile agents. The article will therefore appeal to communities of industrial stakeholders, pharmacists, physicians, food experts, agriculturists, and researchers in related areas such as pharmacology, medicinal chemistry, microbiology, natural product chemistry, food preservation and plant protection.

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Section: Asteraceae Shoot Disc Volatilization Methodsmentioning

confidence: 99%

Section: Asteraceae Shoot Disc Volatilization Methodsmentioning

confidence: 99%

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Volatile Antimicrobial Agents and In Vitro Methods for Evaluating Their Activity in the Vapour Phase: A Review

Houdková

Kokoška

2020

Planta Med

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“…The antibacterial combinatory potential of CCEO in combination with 8-HQ in liquid and vapour phase was determined using a broth volatilization chequerboard method previously developed by our team (Netopilova et al 2018). The technique is based on the combination of classical microdilution chequerboard test and broth microdilution volatilization method (Houdkova et al 2017) allowing determination of combinatory effect of plant volatile agents simultaneously in liquid and vapour phases as well as comparison of MIC and calculation of FIC values in both liquid and solid media.…”

Section: Antimicrobial Assaymentioning

confidence: 99%

In vitro antimicrobial combinatory effect of Cinnamomum cassia essential oil with 8‐hydroxyquinoline against Staphylococcus aureus in liquid and vapour phase

Netopilova

Houdková

Urbanová

et al. 2020

J. Appl. Microbiol.

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Aims The objective of the study was to evaluate the antimicrobial interactions between two volatile agents, Cinnamomum cassia essential oil (CCEO) and 8‐hydroxyquinoline (8‐HQ) against Staphylococcus aureus strains in liquid and vapour phases. Methods and Results In vitro antimicrobial effect of CCEO in combination with 8‐HQ was evaluated against 12 strains of S. aureus by broth volatilization chequerboard method. Results show additive effects against all S. aureus strains for both phases. In several cases, sums of fractional inhibitory concentration values of our test combinations were lower than 0·6, which can be considered as a strong additive interaction. Moreover, composition of CCEO was analysed by gas chromatography–mass spectrometry analysis. In the CCEO, 26 compounds in total were identified, where (E)‐cinnamaldehyde was the predominant compound, followed by cinnamyl acetate, α‐copaene, bornyl acetate and caryophyllene. Conclusions Results showed additive in vitro growth‐inhibitory effect of CCEO and 8‐HQ combination against various standard strains and clinical isolates of S. aureus. Significance and Impact of the Study This is the first report on antibacterial effect of 8‐HQ and CCEO combination in liquid and vapour phases. Results of the study suggest these agents as potential candidates for development of new anti‐staphylococcal applications that can be used in the inhalation therapy against respiratory infections.

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“…currently used in the food industry for food preservation, and these features make them known as one of the most promising EOs (Kachur & Suntres, 2019;Khan et al, 2017;Netopilova, Houdkova, Rondevaldova, Kmet, & Kokoska, 2018). Indeed, several studies have shown that carvacrol and thymol exert an effective antimicrobial effect against S. aureus (Engel, Heckler, Tondo, Daroit, & da Silva Malheiros, 2017;Gallucci et al, 2009;Guarda, Rubilar, Miltz, & Galotto, 2011;Lambert, Skandamis, Coote, & Nychas, 2001;Nostro et al, 2007;Rúa, del Valle, de Arriaga, Fernández-Álvarez, & García-Armesto, 2019).…”

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confidence: 99%

Effect of sub‐lethal treatment of carvacrol and thymol on virulence potential and resistance to several bactericidal treatments of Staphylococcus aureus

Kim

Shin

Kang

et al. 2022

Journal of Food Safety

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This study examined the changes in biofilm‐formation ability, hemolytic/lipase/nuclease/protease activities, and resistance to various bactericidal treatments of Staphylococcus aureus after sublethal treatment with carvacrol and thymol. The minimum inhibitory concentrations (MICs) of carvacrol and thymol for S. aureus were 0.4 and 0.3 mg/ml, respectively, and sublethal concentrations (1/2 and 1/4 MIC) were determined based on these concentrations. Sublethal treatment with carvacrol and thymol did not change the protease and lipase activities but reduced the hemolytic and nuclease activities of S. aureus. After sublethal treatment with carvacrol and thymol, the biofilm‐formation ability of S. aureus was decreased, which was found to be due to the decrease in cell surface hydrophobicity. In addition, after sublethal treatment with carvacrol and thymol, the resistance of S. aureus to heating and malic acid treatments increased, whereas resistance to ultraviolet and hypochlorous acid (HOCl) decreased, and resistance to hydrogen peroxide (H2O2) did not change. The results of this study show that sublethal treatment with carvacrol and thymol can effectively suppress some virulence factors of S. aureus and that applying HOCl or ultraviolet radiation would be an effective subsequent treatment to inactivate S. aureus after sublethal treatment.

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Evaluation of in vitro growth-inhibitory effect of carvacrol and thymol combination against Staphylococcus aureus in liquid and vapour phase using new broth volatilization chequerboard method

Cited by 20 publications

References 24 publications

Volatile Antimicrobial Agents and In Vitro Methods for Evaluating Their Activity in the Vapour Phase: A Review

Volatile Antimicrobial Agents and In Vitro Methods for Evaluating Their Activity in the Vapour Phase: A Review

In vitro antimicrobial combinatory effect of Cinnamomum cassia essential oil with 8‐hydroxyquinoline against Staphylococcus aureus in liquid and vapour phase

Effect of sub‐lethal treatment of carvacrol and thymol on virulence potential and resistance to several bactericidal treatments of Staphylococcus aureus

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