Evaluation of in situ functional activity of casing soils during growth cycle of mushroom (Agaricus bisporus (Lange) Imbach) employing community level physiological profiles (CLPPs)

Abstract

Community level physiological profi les (CLPPs) have been rarely applied to mushroom compost ecosystem, probably for the lack of standardized methodology. Recently, however CLPPs have been employed as a tool to investigate the degree of maturity of compost (Mondini and Insam, 2005, Compost Science and Utilization, 13(1): 27-33). The potential of CLPPs to detect compost maturity test is considerably signifi cant in that it provides sensitivity and the simplicity of the assay. The aim of this work was to investigate the maturity of casing that comprised of farm yard manure and spent compost and infl uence of casing type on the behaviour of bacterial community during the growth cycle of mushroom Agaricus bisporus (Lange) Imbach employing standardized inoculum density and effects of different data interpretation based on the kinetics of colour formation. Casing samples of different age were extracted at a particular dilution and then inoculated in 96 well microtitre plates. Optical density (OD) in well was measured at 590 nm every 24 hours for 5 days. Principal component analysis (PCA) was performed by employing OD values at fi xed average well colour development (AWCD). PCA of fresh samples showed that classifi cation and ordination of samples according to their age were signifi cant with fi xed AWCD.

Impact of Cultivation Substrate and Microbial Community on Improving Mushroom Productivity: A Review

“…In this method, microbial communities are distinguished by analyzing the color of the dye and the number of micropore reactions on the micropore plate. This method has been widely used to characterize the physiological characteristics of microbial communities in different substrates [96][97][98]. However, the application of biological microplate methods in the research of fermentation materials is relatively limited.…”

Microbial Potential of Spent Mushroom Compost and Oyster Substrate in Horticulture: Diversity, Function, and Sustainable Plant Growth Solutions

“…Colonies were transferred to broth media comprising tryptic soy broth (TSB) and King B broth (KBB), respectively, and inoculated at 25 • C with shaking (140 rpm) for 24 h. The 16s rRNA region of all the bacterial isolates was amplified individually by polymerase chain reaction (PCR) with the universal primer pairs 27F (5′-AGAGTTTGATCMTGGCTCAG-3′) and 907R (5′-CCGTCAATTCMTTTRAGTTT-3′), using an initial denaturation step at 94 • C for 3 min, followed by 35 cycles at 94 • C for 45 s, 50 • C for 30 s, and 72 • C for 30 s, then a final extension step at 72 • C for 5 min. The isolated bacterial colonies were screened for production of enzymes protease, phosphatase, cellulase, and xylanase on functional media using a plate assay as described by Choudhary et al (2009).…”

Physical, Chemical and Biological Properties of Casing Soil Used for Milky Mushroom (Calocybe indica P&C) Production

“…However, the cfu of pseudomonads were continued to be higher in clay loam soil even after steaming followed by peat soil (Table 4). Compost or casing soil borne Pseudomonas putida is known to have a positive relationship in the primordial induction and fruiting body formation of button mushroom (Choudhary et al, 2009).…”