Community level physiological profi les (CLPPs) have been rarely applied to mushroom compost ecosystem, probably for the lack of standardized methodology. Recently, however CLPPs have been employed as a tool to investigate the degree of maturity of compost (Mondini and Insam, 2005, Compost Science and Utilization, 13(1): 27-33). The potential of CLPPs to detect compost maturity test is considerably signifi cant in that it provides sensitivity and the simplicity of the assay. The aim of this work was to investigate the maturity of casing that comprised of farm yard manure and spent compost and infl uence of casing type on the behaviour of bacterial community during the growth cycle of mushroom Agaricus bisporus (Lange) Imbach employing standardized inoculum density and effects of different data interpretation based on the kinetics of colour formation. Casing samples of different age were extracted at a particular dilution and then inoculated in 96 well microtitre plates. Optical density (OD) in well was measured at 590 nm every 24 hours for 5 days. Principal component analysis (PCA) was performed by employing OD values at fi xed average well colour development (AWCD). PCA of fresh samples showed that classifi cation and ordination of samples according to their age were signifi cant with fi xed AWCD.
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