Evaluation of Lactobacillus paracasei
 subsp. tolerans
 isolated from Jenyn's sprat (Ramnogaster arcuata
) as probiotic for juvenile rainbow trout Oncorhynchus mykiss
 (Walbaum, 1792)

A complex and dynamic community of microorganisms, play important roles within the fish gastrointestinal (GI) tract. Of the bacteria colonizing the GI tract, are lactic acid bacteria (LAB) generally considered as favorable microorganism due to their abilities to stimulating host GI development, digestive function, mucosal tolerance, stimulating immune response, and improved disease resistance. In early finfish studies, were culture-dependent methods used to enumerate bacterial population levels within the GI tract. However, due to limitations by using culture methods, culture-independent techniques have been used during the last decade. These investigations have revealed the presence of Lactobacillus, Lactococcus, Leuconostoc, Enterococcus, Streptococcus, Carnobacterium, Weissella, and Pediococcus as indigenous species. Numerous strains of LAB isolated from finfish are able to produce antibacterial substances toward different potential fish pathogenic bacteria as well as human pathogens. LAB are revealed be the most promising bacterial genera as probiotic in aquaculture. During the decade numerous investigations are performed on evaluation of probiotic properties of different genus and species of LAB. Except limited contradictory reports, most of administered strains displayed beneficial effects on both, growth—and reproductive performance, immune responses and disease resistance of finfish. This eventually led to industrial scale up and introduction LAB-based commercial probiotics. Pathogenic LAB belonging to the genera Streptococcus, Enterococcus, Lactobacillus, Carnobacterium, and Lactococcus have been detected from ascites, kidney, liver, heart, and spleen of several finfish species. These pathogenic bacteria will be addressed in present review which includes their impacts on finfish aquaculture, possible routes for treatment. Finfish share many common structures and functions of the immune system with warm-blooded animals, although apparent differences exist. This similarity in the immune system may result in many shared LAB effects between finfish and land animals. LAB-fed fish show an increase in innate immune activities leading to disease resistances: neutrophil activity, lysozyme secretion, phagocytosis, and production of pro-inflammatory cytokines (IL-1β, IL-6, IL-8, and TNF-α). However, some LAB strains preferentially induces IL-10 instead, a potent anti-inflammatory cytokine. These results indicate that LAB may vary in their immunological effects depending on the species and hosts. So far, the immunological studies using LAB have been focused on their effects on innate immunity. However, these studies need to be further extended by investigating their involvement in the modulation of adaptive immunity. The present review paper focuses on recent findings in the field of isolation and detection of LAB, their administration as probiotic in aquaculture and their interaction with fish immune responses. Furthermore, the mode of action of probiotics on finfish are discussed.

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“…In a study using rainbow trout (31.25 ± 3.43 g), Lopez Cazorla et al ( 2015 ) tested Lb. paracasei subsp.…”

Section: Lab As Probioticmentioning

confidence: 99%

Lactic Acid Bacteria in Finfish—An Update

et al. 2018

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“…On teleost hosts, only few studies have investigated gut microbiota ontogenesis so far. This is a topic of interest, as the sequence of the initial microbial colonization of the gut has important implications in the yield of aquaculture activities, both in terms of fish growth rate 6 and disease susceptibility 7 . Those parameters are critically dependent upon maturation of fish gut microbiome, which is controlled by the composition of water microbial community 7 and the components of the first feed to the fry 8 .…”

Section: Introductionmentioning

confidence: 99%

Vertically and horizontally transmitted microbial symbionts shape the gut microbiota ontogenesis of a skin-mucus feeding discus fish progeny

Sylvain

Derôme

2017

Sci Rep

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Fish gut microbial communities play key functions for their hosts, but their ontogenesis is poorly understood. Recent studies on the zebrafish suggest that gut symbionts are recruited naturally through horizontal transmission from environmental water. We used an alternative fish model, the discus (Symphysodon aequifasciata), to identify the main factors driving fish gut microbiota ontogenesis. The discus exhibits a unique parenting behavior: both discus parents vertically feed their fry with a cutaneous mucus secretion during three weeks post-hatching. We hypothesized that vertical microbial transmission via parental mucus feeding, along with horizontal transmission of environmental microbial symbionts, helps to shape the taxonomic structure of the discus fry gut microbiota. To assess this premise, we thoroughly documented the gut microbiota ontogenesis of a discus progeny during 100 days post-hatching. The V4 16S rRNA gene was sequenced to assess taxonomic structure of fry gut, parent mucus, and water samples. Our main results suggest that specific microbial symbionts both from the parents skin mucus and environmental water play important roles in shaping the structure of the fry gut microbiota.

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“…As cepas selecionadas ainda foram testadas quanto ao seu perfil de suscetibilidade aos antibióticos (Tabela 4), que demonstrou que o isolado CP08 é resistente à ampicilina, clindamicina e eritromicina; o isolado CP10 foi resistente à ampicilina e gentamicina, e os isolados CP03 e CP20 foram sensíveis a todos os antibióticos testados, corroborando com outros estudos já realizados [47,64,65]. A sensibilidade dos probióticos a antibióticos comerciais é um dos requisitos dos probióticos, pois quando resistentes, os mesmos podem transferir seus genes para a microbiota intestinal do hospedeiro [66], portanto, a ausência de genes de resistência transmissíveis em micro-organismos potencialmente probióticos garante sua aplicação na aquicultura, bem como para o consumo seguro do pescado [67], dessa forma foram excluídas as cepas CP08 e CP10 do estudo.…”

Section: Resultsunclassified

Seleção in vitro de isolados do trato gastrointestinal de Colossoma macropomum, com potencial probiótico

Souza

Garcia

et al. 2023

Sci. Plena

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Os probióticos são utilizados há mais de 30 anos na aquicultura, no entanto, melhores resultados têm sido demonstrados usando cepas espécies-específicas, sustentando a premissa da não utilização de probióticos alóctones. Por este motivo, o objetivo deste estudo foi avaliar o potencial probiótico de cepas de bactérias ácido lácticas provenientes da microbiota intestinal de tambaqui. As bactérias isoladas foram identificadas de acordo com Vieira et al. (2013), através da reação de Gram, morfologia, produção de gás, catalase e hemólise, sendo testadas ainda quanto a sua resistência ao pH, a presença de sais biliares e NaCl, além do antagonismo frente à patógenos e perfil de resistência. Foram isoladas 28 cepas bacterianas, onde apenas quatro (CP03, CP08, CP10 e CP20) foram selecionadas na triagem inicial tendo como base as características das bactérias ácido lácticas. As cepas CP20 e CP03 foram as que melhor responderam aos testes de tolerância aos sais biliares, ao pH e à presença de NaCl respectivamente. A CP03 apresentou antagonismo apenas contra o Staphylococcus aureus e a CP08 e CP20 apresentaram antagonismo contra Escherichia coli, Vibrio harveyi, Pseudomonas aeroginosa e Aeromonas hydrophila. Apenas a CP03 e CP020 foram sensíveis a todos os antibióticos testados. As cepas bacterianas CP03 e CP020 exibiram propriedades probióticas significativas, apresentando boa viabilidade aos testes de resistência in vitro, assim como capacidade inibitória de patógenos. Dessa maneira sendo recomendadas como possível probiótico multiespécie.

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Evaluation of Lactobacillus paracasei subsp. tolerans isolated from Jenyn's sprat (Ramnogaster arcuata ) as probiotic for juvenile rainbow trout Oncorhynchus mykiss (Walbaum, 1792)

Cited by 12 publications

References 50 publications

Lactic Acid Bacteria in Finfish—An Update

Lactic Acid Bacteria in Finfish—An Update

Vertically and horizontally transmitted microbial symbionts shape the gut microbiota ontogenesis of a skin-mucus feeding discus fish progeny

Seleção in vitro de isolados do trato gastrointestinal de Colossoma macropomum, com potencial probiótico

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