Evaluation ofin vivocytogenetic toxicity of europium hydroxide nanorods (EHNs) in male and female Swiss albino mice

Abstract

Our group already demonstrated that europium hydroxide nanorods (EHNs) show none or mild toxicity in C57BL/6 mice even at high dose and exhibited excellent pro-angiogenic activity towards in vitro and in vivo models. In the present study, we evaluated the in vivo cytogenetic toxicity of intraperitoneally administered EHNs (12.5-250 mg/kg/b.w.) in male and female Swiss albino mice by analyzing chromosomal aberrations (CAs), mitotic index (MI), micronucleus (MN) from bone marrow and peripheral blood. Furthermore, we performed the cytogenetic toxicity study of EHNs towards Chinese hamster ovary (CHO) cells, in order to compare with the in vivo results. The results of CA assay of mice treated with EHNs (12.5-125 mg/kg/b.w.) showed no significant change in the formation of aberrant metaphases compared to the control group. Also, there was no significant difference in the number of dividing cells between the control group and EHNs-treated groups observed by MI study, suggesting the non-cytotoxicity of EHNs. Additionally, FACS study revealed that EHNs do not arrest cells at any phase of cell cycle in the mouse model. Furthermore, MN test of both bone marrow and peripheral blood showed no significant differences in the induction of MNs when compared with the control group. In vitro results from CHO cells also support our in vivo observations. Considering the role of angiogenesis by EHNs and the absence of its genotoxicity in mouse model, we strongly believe the future application of EHNs in treating various diseases, where angiogenesis plays an important role such as cardiovascular diseases, ischemic diseases and wound healing.

Synthesis, characterization and in vitro anticancer, DNA binding and cleavage studies of Mn (II), Co (II), Ni (II) and Cu (II) complexes of Schiff base ligand 3‐(2‐(1‐(1H‐benzimidazol‐2‐yl)ethylidene)hydrazinyl)quinoxalin‐2(1H)‐one and crystal structure of the ligand

“…In vitro anticancer activity studies of the Ni (II) and Cu (II) complexes of BZHQO were investigated using MTT assay, with cell lines HeLa, B16‐F10, SKOV3, MCF7 and CHO‐K1. The results are expressed as IC 50 values that represent the concentration of complex required for the inhibition of the growth of 50% of cells in vitro .…”

“…Finally the absorbance of the plates was measured using a Synergy H1 multi‐mode plate reader (USA). Mitomycin‐C for normal cells and doxorubicin for cancer cells were used as positive controls for comparison …”

Synthesis and evaluation of anti-oxidant and cytotoxic activities of novel 10-undecenoic acid methyl ester based lipoconjugates of phenolic acids

“…The cytotoxicity assay (MTT) was evaluated for all test compounds as described in our earlier work [25]. Five different cancer cell lines viz., MDA-MB-231, breast cancer (ATCC ® HTB-26™); SKOV3, ovarian cancer (ATCC ® HTB-77™); MCF7, breast cancer (ATCC ® HTB-22™); DU 145, prostate cancer (ATCC ® HTB-81™); HepG2, liver hepatocellular carcinoma (ATCC ® HB-8065™) were obtained from the ATCC (Bethesda, MD, USA) and maintained in DMEM supplemented with 10% FBS, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin at 37 °C in a 5% CO 2 incubator.…”

Molecular Engineering of Tetracyclic 2,3‐Dihydro‐1H‐benzo[2,3]‐benzofuro[4,5‐e][1,3]oxazine Derivatives: Evaluation for Potential Anticancer Agents

“…Cytotoxicity assay (MTT) was evaluated for all the test compounds ( MCV‐1–31 ) as per our earlier published work . Three different cancer cell lines, viz.…”

“…Finally, the absorbance of the plates were measured using a Synergy H1 multi‐mode plate reader, USA. Doxorubicin and DMSO were used as positive and vehicle control, respectively, for the comparison of cytotoxicity activity .…”