Synthesis, characterization and in vitro anticancer, DNA binding and cleavage studies of Mn (II), Co (II), Ni (II) and Cu (II) complexes of Schiff base ligand 3‐(2‐(1‐(1H‐benzimidazol‐2‐yl)ethylidene)hydrazinyl)quinoxalin‐2(1H)‐one and crystal structure of the ligand

Abstract

A Schiff base ligand, 3‐(2‐(1‐(1H‐benzimidazol‐2‐yl)ethylidene)hydrazinyl)quinoxalin‐2(1H)‐one (BZHQO), has been synthesized by the condensation of 3‐hydrazinylquinoxalin‐2(1H)‐one and 1‐(1H‐benzoimidazol‐2‐yl) ethanone and characterized using spectral and single‐crystal X‐ray analyses. Mn (II), Co (II), Ni (II) and Cu (II) complexes of the BZHQO ligand have been synthesized and characterized. The interactions of the ligand and its metal complexes with calf thymus DNA have been investigated using absorption spectroscopy and the intrinsic binding constant has been evaluated. Agarose gel electrophoresis has been performed to study the abilities of the ligand and its metal complexes to cleave supercoiled pBR322 DNA into nicked circular form. In vitro anticancer activities of the Cu (II) and Ni (II) complexes have been investigated by MTT assay, using the cell lines HeLa, B16‐F10, SKOV3 and MCF7. The Cu (II) complex has been found to be active against HeLa, B16‐F10 and MCF7, while the Ni (II) complex has been found to be active against MCF‐7.

A selective CuII complex with 4-fluorophenoxyacetic acid hydrazide and phenanthroline displays DNA-cleaving and pro-apoptotic properties in cancer cells

“…Therefore, complex 1 was selected for further studies. Additionally, when compared to other Cu II complexes containing analogous ligands, complex 1 has already been shown to stand out as one of the most active copper-based compounds 25 , 26 with high selectivity 22 , as it can be confirmed by the present work.…”

Retracted: A new Co(II)‐coordination polymer: Photocatalytic studies and treatment values on mania by reducing the ErbB4 expression on the noradrenergic neurons

“…[15] Since the first reported studies on the biological activity of cobalt complexes in 1952, many cobalt complexes of biological interest have been reported, with the most structurally characterized showing antitumor, antiproliferative, antimicrobial, and antifungal activity. [16][17][18][19][20] In our experiment, a novel CP based on Co(II) has been generated in the existence of a second N-donor ligand 3,3 0 -bipyridine (3,3 0 -bpy) utilizing 4,4 0 ,4 00 -phosphanetriyltribenzoic acid (H 3 tpp) as the tritopic connector under conditions of solvothermal reaction, and its chemical formula is [Co 3 (tpp) 2 (3,3 0 -bpy)]Á 7DMF (1). The product acquired was completely investigated using powder X-ray diffraction (PXRD), thermogravimetric analyses, infrared radiation (IR) spectra, and elemental analysis (EA), as well as the diffraction of a single-crystal X-ray.…”

Chromone‐Appended Zn(II) tRNA‐Targeted Potential Anticancer Chemotherapeutic Agent: Structural Details, in vitro ct‐DNA/tRNA Binding, Cytotoxicity Studies And Antioxidant Activity

“…Gel electrophoresis of plasmid DNA resulted in the migration of fastest moving supercoiled form, Form I to relaxed nicked form, Form II, along with the appearance of slow-moving linearized form, Form III of DNA migrating between supercoiled and nicked forms, which indicated lethal double-stranded cleavage of DNA (Figure S9). [56] The concentration-dependent DNA cleavage assay was performed to evaluate the affinity of complex 1 towards supercoiled (SC) DNA in the absence of any reducing agent. Moreover, complex 1 showed efficient cleavage at the micromolar concentration of 15 μM (lane 4) i. e., conversion of Form I to Form II to Form III takes place.…”