EVALUATION OF HYPOALLERGENICITY OF VARIOUS INFANT FORMULA USING TNFα RELEASED FROM MONONUCLEAR CELLS OF COW'S MILK ALLERGIC CHILDREN.

Heyman, Martine; Benlounes, Nadia; Candalh, Céline; Dupont, Christophe

doi:10.1097/00005176-199704000-00083

Cited by 3 publications

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“…The 24‐hour TNF‐α test was used to screen the immunogenicity of extensively hydrolyzed infant formulae or amino acid‐based formula, by using mononuclear cells from infants with allergic to intact milk proteins or to milk protein hydrolysates, who had development of intestinal or skin symptoms (88). The results confirmed that TNF‐α secretion by mononuclear cells from infants with active cow's milk allergy is specifically increased in the presence of cow's milk proteins and further indicate that the TNF‐α test could be used to identify potentially active peptides still present in extensively hydrolyzed formulas.…”

Section: Testing Allergenicity Of Cow's Milk Substitutesmentioning

confidence: 99%

Food Protein‐Induced Enterocolitis Syndrome: Laboratory Perspectives

Dupont,

Heyman,

Simpson

et al. 2000

J. pediatr. gastroenterol. nutr.

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The expression of food protein allergy in man is very heterogeneous, varies with the age of the subject and is to a certain extent genetically determined. Skin prick tests with standardized food extracts are a sensitive method for detection of immunoglobulin E bound to reactive cells such as mast cells. Various tests on cellular immunity have been developed, especially because T‐cell mediated reactions are considered to play a role in mainly delayed gastrointestinal reactions to cow's milk proteins. Food allergy may involve the entire gut, from mouth to rectum, including the esophagus. Abnormalities in intestinal permeability are the hallmarks of the inflamed gut, and may contribute to diagnosis of food induced enteropathy. What determines the characteristics of the intestinal inflammatory response is largely the cytokine responses triggered by the pathologic mechanism, whatever its origin, in the stomach, the small intestine, and the colon. A so‐called T‐helper type 2 response is characteristic of the allergic subject. A secretion of tumor necrosis factor‐α alpha by blood cells of children allergic to milk was shown. All means of investigation may help in analyzing food substitutes for allergic infants.

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Section: Testing Allergenicity Of Cow's Milk Substitutesmentioning

confidence: 99%

Food Protein‐Induced Enterocolitis Syndrome: Laboratory Perspectives

Dupont,

Heyman,

Simpson

et al. 2000

J. pediatr. gastroenterol. nutr.

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Evaluation of the impact of food technology on the allergenicity of cow’s milk proteins

Heyman

1999

Proc. Nutr. Soc.

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The quantitative evaluation of the allergenicity of food proteins and the clinical tolerance towards antigens are problems the food industry and the clinicians have to face. The allergenicity of a protein depends on multiple factors, including the stability to digestion and the interaction with the intestinal environment. In addition to the possible reduction in allergenicity by technological treatments such as heat and enzymic hydrolysis, the complex interactions existing between the antigens, the intestinal epithelium and the underlying immune system, as well as the individual susceptibility to the sensitizing epitopes, have to be taken into account. Indeed, the intestinal cells are able to take up and process proteins, and possibly to present them directly to mucosal lymphocytes. On the other hand, pathophysiological conditions can modify the interactions between food antigens and the immune system. A large number of methods has been developed to assess the residual antigenicity of food proteins, based on the various immune responses leading to intestinal or extradigestive pathologies. Thus, the difficulty in measuring the residual allergenicity of hypoallergenic formulas is partly due to the physiology of the gastrointestinal tract, since an intricate network of interactions between enterocytes and immune cells governs the development of the immune response to food antigens. Résumé L'évaluation de l'allergénicité des aliments et leur bonne tolérance clinique est une question touchant à la fois les industriels de l'agro-alimentaire et les cliniciens. L'allergénicité d'une protéine dépend de multiples facteurs parmi lesquels la résistance à la digestion, les interactions avec le tractus digestif et les facteurs environnementaux. L'allergénicité d'un produit alimentaire peut être modifiée non seulement par les traitements technologiques industriels, mais aussi par le tractus gastrointestinal. La susceptibilité individuelle aux epitopes peptidiques formés est également un facteur primordial. En effet, les interactions complexes existant entre les antigènes, l'épithelium intestinal et le système immunitaire muqueux peuvent conduirent à des réponses immunitaires différentes selon les individus. De nombreuses méthodes existent pour mesurer l'antigénicité résiduelle des aliments, basées sur la nature des différentes réactions immunitaires anormales conduisant à une pathologie intestinale ou extradigestive. La difficulté de mesurer l'allergénicité résiduelle de formules hypoallergéniques repose donc sur les relations complexes entre les antigènes alimentaires, l'épithelium intestinal et le système immunitaire muqueux. Adverse reactions to food are relatively frequent, and affect mainly the digestive tract but also many other organs, explaining the variety of symptoms observed. They are divided into toxic and non-toxic reactions, the latter being divided into immune-mediated (allergy) and non-immunemediated (intolerance) reactions. Food allergy develops when the intestinal immune system does not respond normally to th...

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Food Protein-Induced Enterocolitis Syndrome: Laboratory Perspectives

Dupont

Heyman

2000

Journal of Pediatric Gastroenterology and Nutrition

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The expression of food protein allergy in man is very heterogeneous, varies with the age of the subject and is to a certain extent genetically determined. Skin prick tests with standardized food extracts are a sensitive method for detection of immunoglobulin E bound to reactive cells such as mast cells. Various tests on cellular immunity have been developed, especially because T-cell mediated reactions are considered to play a role in mainly delayed gastrointestinal reactions to cow's milk proteins. Food allergy may involve the entire gut, from mouth to rectum, including the esophagus. Abnormalities in intestinal permeability are the hallmarks of the inflamed gut, and may contribute to diagnosis of food induced enteropathy. What determines the characteristics of the intestinal inflammatory response is largely the cytokine responses triggered by the pathologic mechanism, whatever its origin, in the stomach, the small intestine, and the colon. A so-called T-helper type 2 response is characteristic of the allergic subject. A secretion of tumor necrosis factor-alpha alpha by blood cells of children allergic to milk was shown. All means of investigation may help in analyzing food substitutes for allergic infants.

show abstract

EVALUATION OF HYPOALLERGENICITY OF VARIOUS INFANT FORMULA USING TNFα RELEASED FROM MONONUCLEAR CELLS OF COW'S MILK ALLERGIC CHILDREN.

Cited by 3 publications

References 0 publications

Food Protein‐Induced Enterocolitis Syndrome: Laboratory Perspectives

Food Protein‐Induced Enterocolitis Syndrome: Laboratory Perspectives

Evaluation of the impact of food technology on the allergenicity of cow’s milk proteins

Food Protein-Induced Enterocolitis Syndrome: Laboratory Perspectives

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