Food Protein-Induced Enterocolitis Syndrome: Laboratory Perspectives

Dupont, Christophe; Heyman, Martine

doi:10.1097/00005176-200001001-00008

Cited by 34 publications

(23 citation statements)

References 80 publications

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“…Special attention has focused on TNF-α, and IL-10 and IL-12 as key regulators of allergic diseases (24, 26), where IL-12 and TNF-α promote Th1 responses and cellular immunity, whereas IL-10 suppresses Th1 activities and stimulates Th2 and humoral immune responses. A number of laboratory studies have been proposed to identify specific proteins responsible for allergic disorders related to CMPA (15,16,22,29,30). The possibility to use TNF-α, IL-10, and IL-12 in laboratory predictive tests for CMPA has been suggested in various studies, given the importance for children not to be exposed to the potentially dangerous reintroduction of the antigen (3, 22).…”

Section: Discussionmentioning

confidence: 99%

Levels of inflammatory cytokines from peripheral blood mononuclear cells of children with cow’s milk protein allergy

et al. 2018

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Aim: The aim of the study was to investigate the level of cytokines in cultures of cow's milk protein-stimulated peripheral blood mononuclear cells of patients with cow's milk protein allergy. Material and Methods:Eleven children with cow's milk protein allergy and 11 non-allergic controls were studied. Their peripheral blood mononuclear cells were cultured alone and in the presence of cow's milk α-lactalbumin; β-lactoglobulin; αS 1, αS 2, β, and κ-casein fraction mixtures; and a cow's protein mixture from whole milk. Production of cytokines, tumor necrosis factor-α, interleukin-10, and interleukin-12 were determined in culture supernatants.Results: In cow's milk protein-stimulated peripheral blood mononuclear cell cultures of children with cow's milk protein allergy, tumor necrosis factor-α, interleukin-10, and interleukin-12 production was significantly higher than in non-allergic controls (p<0.05). No difference in cytokine production was found between cultures obtained from unstimulated peripheral blood mononuclear cell cultures of both cow's milk protein allergy and non-allergic controls. Conclusions:The findings of this preliminary study align with data from the literature suggesting that the investigation of tumor necrosis factor-α, interleukin-10, and interleukin-12 in cow's milk protein-stimulated peripheral blood mononuclear cell cultures of children may be taken in further consideration to explore whether they might have a predictive role for cow's milk protein allergy. Further studies are therefore needed to extensively investigate this issue. Keywords: Challenge, cow milk, cytokines, immunoglobulin, interleukin, tumor necrosis factor Original Article IntroductionCow's milk protein allergy (CMPA) is an adverse immunologic response to cow's milk proteins (1). In particular, four casein fractions (αS 1, αS 2, β, and κ-casein) and two whey proteins (α-lactalbumin and β-lactoglobulin) are considered the most important allergenic proteins contained in cow's milk (2). Cow's milk protein allergy is one of the most common forms of food allergy in infants aged below 24 months in economically advanced countries (3) and its prevalence in this age group is estimated as 2-7.5%, whereas 5 to 15% of infants show symptoms suggesting adverse reactions to cow's milk components (4,5). Preventing the onset of inappropriate elimination diets in children with CMPA is important (4). In fact, the only effective treatment for CMPA is the total avoidance of cows' milk, which has to be replaced by appropriate substitutes (6), including soy formulas or casein, extensively hydrolyzed whey formulas or other mammalian milks such as goat's milk (7), which is frequently used although several studies raised concerns regarding its tolerability and safety, particularly in children (3,8,9).The immune reaction to cow's milk proteins is determined by immunoglobulin (Ig)-E or non-IgE-mediated type of response (10). Currently, the diagnosis relies both on IgE tests, such as the skin prick test, and measurement of serum-specific Ig...

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Section: Discussionmentioning

confidence: 99%

Levels of inflammatory cytokines from peripheral blood mononuclear cells of children with cow’s milk protein allergy

et al. 2018

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“…Most ASD children exhibit no immediate GI/systemic symptoms with intake of DPs, but develop GI symptoms several hours after ingestion of DPs. In non IgE-mediated immune responses to DPs, production of inflammatory cytokines, especially TNF-· in the gut mucosa, appears to play a vital role in its pathogenesis [7,11,21,22]. In DPsensitive enteropathy, local immune reactions to DPs in the gut augment production of inflammatory cytokines, leading to chronic gut inflammation and increase in the gut permeability.…”

Section: Discussionmentioning

confidence: 99%

“…We assessed immune reactivity to DPs by peripheral blood mononuclear cells (PBMCs) from ASD children by measuring production of IFN-Á, IL-5, and TNF-· against common DPs. Both IFN-Á, a type 1 T cell (T1) cytokine, and TNF-· are implicated with CMP intolerance [1,[10][11][12]. IL-5 was measured as a representative type 2 T cell (T2) cytokine [13].…”

Section: Introductionmentioning

confidence: 99%

Innate Immunity Associated with Inflammatory Responses and Cytokine Production against Common Dietary Proteins in Patients with Autism Spectrum Disorder

Jyonouchi

Sun

Itokazu³

2002

Neuropsychobiology

204

121

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Objectives: Children with autism spectrum disorder (ASD) frequently reveal various gastrointestinal (GI) symptoms that may resolve with an elimination diet along with apparent improvement of some of the behavioral symptoms. Evidence suggests that ASD may be accompanied by aberrant (inflammatory) innate immune responses. This may predispose ASD children to sensitization to common dietary proteins (DP), leading to GI inflammation and aggravation of some behavioral symptoms. Methods: We measured IFN-γ, IL-5, and TNF-α production against representative DPs [gliadin, cow’s milk protein (CMP), and soy] by peripheral blood mononuclear cells (PBMCs) from ASD and control children [those with DP intolerance (DPI), ASD siblings, and healthy unrelated children]. We evaluated the results in association with proinflammatory and counter-regulatory cytokine production with endotoxin (LPS), a microbial product of intestinal flora and a surrogate stimulant for innate immune responses. Results: ASD PBMCs produced elevated IFN-γ and TNF-α, but not IL-5 with common DPs at high frequency as observed in DPI PBMCs. ASD PBMCs revealed increased proinflammatory cytokine responses with LPS at high frequency with positive correlation between proinflammatory cytokine production with LPS and IFN-γ and TNF-α production against DPs. Such correlation was less evident in DPI PBMCs. Conclusion: Immune reactivity to DPs may be associated with apparent DPI and GI inflammation in ASD children that may be partly associated with aberrant innate immune response against endotoxin, a product of the gut bacteria.

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“…These are dietary protein enterocolitis syndrome, dietary protein proctitis/ proctocolitis syndrome, dietary protein enteropathy and the well-documented coeliac disease 5 . In proctitis / proctocolitis syndrome, manifestations are seen in early infancy and includes blood streaked stools and anaemia 1 .…”

Section: Exclusively Cell-mediated Disorders (Non-ige Mediated)mentioning

confidence: 99%

Some aspects of food allergy

Lamabadusuriya

2009

Sri Lanka J Child Health

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Food Protein-Induced Enterocolitis Syndrome: Laboratory Perspectives

Cited by 34 publications

References 80 publications

Levels of inflammatory cytokines from peripheral blood mononuclear cells of children with cow’s milk protein allergy

Levels of inflammatory cytokines from peripheral blood mononuclear cells of children with cow’s milk protein allergy

Innate Immunity Associated with Inflammatory Responses and Cytokine Production against Common Dietary Proteins in Patients with Autism Spectrum Disorder

Some aspects of food allergy

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