Evaluation of genotoxic activity of maleic hydrazide, ethyl methane sulfonate, and N-nitroso diethylamine in Tradescantia

Alvarez-Moya, Carlos; Santerre-Lucas, Anne; Zúñiga‐González, Guillermo Moisés; Torres-Bugarı́n, Olivia; Padilla-Camberos, Eduardo; Feria‐Velasco, Alfredo

doi:10.1590/s0036-36342001000600007

Cited by 18 publications

(24 citation statements)

References 29 publications

(64 reference statements)

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“…The results of treatment with lead nitrate observed here concur with those reported by other authors for diverse biological systems [33][34][35][36]) and provide evidence for the toxic effects of lead on exposed cells [37].…”

Section: Chromosomal Aberrations and Micronuclei Inductionsupporting

confidence: 91%

Cytotoxic and Genotoxic Effects of cis-Tetraammine(oxalato)Ruthenium(III) Dithionate on the Root Meristem Cells of Allium cepa

Pereira

Vilanova-Costa

Lima

et al. 2008

Biol Trace Elem Res

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Ruthenium complexes have attracted much attention as possible building blocks for new transition-metal-based antitumor agents. The present study examines the mitotoxic and clastogenic effects induced in the root tips of Allium cepa by cis-tetraammine(oxalato)ruthenium(III) dithionate {cis-[Ru(C(2)O(2))(NH(3))(4)](2)(S(2)O(6))} at different exposure durations and concentrations. Correlation tests were performed to determine the effects of the time of exposure and concentration of ruthenium complex on mitotic index (MI) and mitotic aberration index. A comparison of MI results of cis-[Ru(C(2)O(2))(NH(3))(4)](2)(S(2)O(6)) to those of lead nitrate reveals that the ruthenium complex demonstrates an average mitotic inhibition eightfold higher than lead, with the frequency of cellular abnormalities almost fourfold lower and mitotic aberration threefold lower. A. cepa root cells exposed to a range of ruthenium complex concentrations did not display significant clastogenic effects. Cis-tetraammine(oxalato)ruthenium(III) dithionate therefore exhibits a remarkable capacity to inhibit mitosis, perhaps by inhibiting DNA synthesis or blocking the cell cycle in the G2 phase. Further investigation of the mechanisms of action of this ruthenium complex will be important to define its clinical potential and to contribute to a novel and rational approach to developing a new metal-based drug with antitumor properties complementary to those exhibited by the drugs already in clinical use.

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Section: Chromosomal Aberrations and Micronuclei Inductionsupporting

confidence: 91%

Cytotoxic and Genotoxic Effects of cis-Tetraammine(oxalato)Ruthenium(III) Dithionate on the Root Meristem Cells of Allium cepa

Pereira

Vilanova-Costa

Lima

et al. 2008

Biol Trace Elem Res

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“…Lower concentrations cause less severe genetic damage, producing more visible and apparently longer tails. This might explain the apparent paradox, as reported by Alvarez-Moya et al (2001).…”

Section: Discussionmentioning

confidence: 82%

“…The mixture was then filtered through a nylon mesh with a pore opening of 80 µm. The nuclei were separated by centrifugation at 3000 rpm (4°C) for 3 min and then washed 3 times in 5 mL phosphate-buffered saline (0.9% NaCl), resuspended in 200 µL phosphate buffer (Alvarez-Moya et al, 2001), and stored until use.…”

Section: Obtaining Tradescantia Nucleimentioning

confidence: 99%

“…One of the most used methods is the alkaline comet assay that was developed by Singh et al (1988), which can detect breaks in the DNA strands, alkali-sensitive sites, and incomplete repair sites (Nacci et al, 1996;Belpaeme et al, 1998). The assay (Koppen and Verschaeve, 1996) and its effectiveness were shown by using the nuclei of Tradescantia (Alvarez-Moya et al, 2001); additionally, the assay could be used to examine the genotoxic activity of several natural products.…”

Section: Introductionmentioning

confidence: 99%

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Assessing the genotoxicities of sparteine and compounds isolated from Lupinus mexicanus and L. montanus seeds by using comet assay

Silva¹,

Alvarez²,

Garcia³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. The genus Lupinus is widely distributed. Its seeds are used for animal and human food, and Lupinus possesses pharmacological potential because of its high content of quinolizidine alkaloids and flavonoids; however, there is little available information about its genotoxicity. We used the comet assay and staminal nuclei of Tradescantia (clone 4430) to evaluate the in vitro genotoxicity of 4 concentrations (0.01, 0.1, 0.5, and 1.0 mM) of alkaloid extracts of Lupinus mexicanus and Lupinus montanus, flavonoids of L. mexicanus, and commercial sparteine; nitrosodiethylamine was used as a positive control and untreated nuclei were used as a negative control. All concentrations of L. mexicanus and L. montanus showed significant genotoxic activity (P ≤ 0.05). A similar behavior was observed for flavonoid extracts of L. montanus except the 1.0 mM concentration. Sparteine showed genotoxic activity only at 0.5 mM. The order of Genotoxicities of Lupinus extracts genotoxicity of the compounds studied was as follows: L. mexicanus > L. montanus > flavonoids of L. montanus > sparteine. There is evident genotoxic activity in the compounds that were studied, particularly at lower concentrations (0.01 and 0.1 mM). Given the limited information about the genotoxicity of the compounds of L. mexicanus and L. montanus, further studies are necessary.

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“…Thus, characterising the mutagenic properties of the effluent is crucial to complement the chemical and biological analyses and provide evidences of possible genotoxic agents. The detection of the genotoxic activities of such agents may be determined through the test of mutation in Tradescantiaa simple low cost procedure that could provide the results in approximately 15 days -being employed for the in situ or in laboratory monitoring (Ma et al 1982;Alverez-Moya et al 2001). The present study aimed at the assessment of genotoxicity of raw wastes and effluents of a treatment system of swine wastes in clones of Tradescantia.…”

Section: Introductionmentioning

confidence: 99%

Genotoxicity of swine effluents

Techio

Stolberg

Kunz³

et al. 2011

Water Science and Technology

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This study aimed at the investigation of genotoxic effects of swine effluents from different stages of a treatment system for swine wastes through bioassay of stamen hairs and micronuclei in Tradescantia (clone BNL 4430). No significant differences (p≥0.05) regarding the genic mutations were found in the bioassay of stamen hairs, independently of the effluent analysed. For the genotoxicity test with micronuclei, the plants exposed to raw wastes, to sludge, and to effluent of the biodigester have presented higher rates of chromosomal damages (micronuclei), with significant differences in relation to the control group and other effluent of the waste treatment system (p≤0.05). The association between the chemical parameters and the genotoxicity data have shown that the variables COD and TKN have presented significant correlation (p≤0.05) with the number of mutagenic events in the tetrads.

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Evaluation of genotoxic activity of maleic hydrazide, ethyl methane sulfonate, and N-nitroso diethylamine in Tradescantia

Cited by 18 publications

References 29 publications

Cytotoxic and Genotoxic Effects of cis-Tetraammine(oxalato)Ruthenium(III) Dithionate on the Root Meristem Cells of Allium cepa

Cytotoxic and Genotoxic Effects of cis-Tetraammine(oxalato)Ruthenium(III) Dithionate on the Root Meristem Cells of Allium cepa

Assessing the genotoxicities of sparteine and compounds isolated from Lupinus mexicanus and L. montanus seeds by using comet assay

Genotoxicity of swine effluents

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