Evaluation of enzyme‐linked immunosorbent assays based on monoclonal antibodies for the serology and antigen detection in canine parvovirus infections

Rimmelzwaan, Guus F.; Juntti, N.; Klingeborn, B.; Groen, Jan; Uytdehaag, Fons G. C. M.; Osterhaus, A. D. M. E.

doi:10.1080/01652176.1990.9694236

Cited by 12 publications

(13 citation statements)

References 7 publications

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“…As has also been documented previously, the sensitivity of indirect ELISA systems for the detection of serum antibodies to CPV, CCV and rotavirus was about 2-10 times higher than conventional methods such as HI, VN and CF (Ghose et al, 1978;Osterhaus et al, 1980a;Fiscus et al, 1985;Rimmelzwaan et al, 1990). Results obtained with the same sera in the CTB-ELISA system, which is based on a different principle, correlated well with those obtained in the indirect ELISA system.…”

Section: Discussionsupporting

confidence: 80%

“…The ELISA system used for the detection of viral antigens in the feces of dogs has been used previously for the detection of CPV antigen, and proved to correlate well with results obtained in the HA assay. This confirmed both the specificity and the sensitivity of this ELISA Teramoto et al, 1984;Rimmelzwaan et al, 1990). The specificities of the antigen ELISAs were further confirmed by using the appropriate specific antiviral antibodies in CTB-ELISAs with all the positive fecal samples, and by showing that all the samples from SPF dogs were negative.…”

Section: Discussionsupporting

confidence: 71%

“…Indirect ELISA for the detection of CPV-specific antibodies. This has been described previously (Rimmelzwaan et al, 1990). Briefly, plates were coated with the CPV-specific monoclonal antibodies H-1 and H-2 as described above.…”

Section: Elisa Techniques For the Detection Of Virus-specific Antibodiesmentioning

confidence: 99%

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The use of enzyme-linked immunosorbent assay systems for serology and antigen detection in parvovirus, coronavirus and rotavirus infections in dogs in The Netherlands

Rimmelzwaan

Groen

Egberink

et al. 1991

Veterinary Microbiology

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Complex trapping blocking (CTB) enzyme-linked immunosorbent assays (ELISAs) and indirect ELISAs for the detection of antibodies to canine parvovirus (CPV), canine coronavirus (CCV) and rotavirus in sera of dogs were established. Double antibody sandwich ELISAs for the detection of CPV-, CCV- and rotavirus antigens in fecal samples were also developed. Both the serological and antigen-detection ELISAs were used to screen samples from dogs in The Netherlands, with or without a history of acute diarrhea. It was shown that the results of the respective serological ELISAs correlated well and that CPV was the major cause of virus-induced acute diarrhea in dogs in The Netherlands.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionsupporting

confidence: 71%

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The use of enzyme-linked immunosorbent assay systems for serology and antigen detection in parvovirus, coronavirus and rotavirus infections in dogs in The Netherlands

Rimmelzwaan

Groen

Egberink

et al. 1991

Veterinary Microbiology

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“…PHE-CoV can be isolated from the nasal cavity, trachea, brain, and lungs of diseased or healthy pigs [34][35][36] and the brain had the highest detection rate by nested PCR and RT-PCR [15]. Because of RT-PCR and ELISA are also widely applied in clinical practice due to its high sensitivity and specificity [37,38]. Thus, a lot of brain tissue samples were collected from deceased piglets with suspected PHE-CoV infection, and using RT-PCR and ELISA as reference test, the relative specificity and sensitivity of the immunochromatographic strip were determined to be 100% and 97.78%, respectively.…”

Section: Discussionmentioning

confidence: 99%

Development and evaluation of an immunochromatographic strip for rapid detection of porcine hemagglutinating encephalomyelitis virus

Chen

Zhao

Song

et al. 2012

Virol J

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BackgroundThe incidence of PHE among pigs in many countries is on the rise, and it has caused great economic losses to the pig industry. Therefore, the development of a sensitive, specific, and easily-performed assay is crucial for the rapid detection and surveillance of PHE-CoV infection and transmission.ResultsAn immunochromatographic strip was developed for the detection of PHE-CoV. The colloidal gold-labeled MAb 4D4 was used as the detection reagent, and the MAb 1E2 and goat anti-mouse IgG coated the strip's test and control lines, respectively. The immunochromatographic strip was capable of specifically detecting PHE-CoV with a HA unit of 2 within 10 min. Storage of the strips at room temperature for 6 months or at 4°C for 12 months did not change their sensitivity or specificity. Using RT-PCR as a reference test, the relative specificity and sensitivity of the immunochromatographic strip were determined to be 100% and 97.78%, respectively. There was an excellent agreement between the results obtained by RT-PCR and the immunochromatographic strips (kappa = 0.976). Additionally, there was a strong agreement between the sandwich enzyme-linked immunosorbent assay (ELISA) and immunochromatographic strips (Kappa = 0.976). When the immunochromatographic strips were used for diagnosing PHE-CoV infection in the Jilin Province, the PHE-CoV-positive rate ranged from 61.54% in the Jilin district to 17.95% in the Songyuan district.ConclusionsBased on its high specificity, sensitivity, and stability, the immunochromatographic strip would be suitable for on-site detection of PHE-CoV for surveillance and epidemiological purposes.

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“…Monoclonal antibodies (mAbs) to CPV were developed as described previously (Rimmelzwaan et al . 1990).…”

Section: Methodsmentioning

confidence: 99%

A Comparative Study of a New Rapid and One‐Step Test for the Detection of Parvovirus in Faeces from Dogs, Cats and Mink

Esfandiari

Klingeborn

2000

Journal of Veterinary Medicine, Series B

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A one-step immunochromatographic test, based on the use of monoclonal antibodies, was developed for the detection of canine parvovirus (CPV) in dog faeces. In addition to canine parvovirus the test can also be used for the diagnosis of infections with viruses causing parvovirus enteritis in cats (feline panleukopenia virus) and mink (mink enteritis virus). Four hundred and forty-three faecal samples were evaluated by comparative testing between this one-step test and three different enzyme-linked immunosorbent assays (ELISA) in Sweden, Denmark and The Netherlands. The result of the evaluation showed an overall relative sensitivity and specificity of 95.8 and 99.7%, respectively. Furthermore, the comparative testing of 83 dog samples in Germany between the one-step test and an immune electron microscopy (IEM) agreed to 85.5%. The sensitivity and specificity were 83.9 and 88.9%, respectively. These results show that the one-step test is a rapid, simple, reproducible and sensitive diagnostic test for the detection of parvovirus in faecal samples of dogs, cats and mink.

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Evaluation of enzyme‐linked immunosorbent assays based on monoclonal antibodies for the serology and antigen detection in canine parvovirus infections

Cited by 12 publications

References 7 publications

The use of enzyme-linked immunosorbent assay systems for serology and antigen detection in parvovirus, coronavirus and rotavirus infections in dogs in The Netherlands

The use of enzyme-linked immunosorbent assay systems for serology and antigen detection in parvovirus, coronavirus and rotavirus infections in dogs in The Netherlands

Development and evaluation of an immunochromatographic strip for rapid detection of porcine hemagglutinating encephalomyelitis virus

A Comparative Study of a New Rapid and One‐Step Test for the Detection of Parvovirus in Faeces from Dogs, Cats and Mink

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