The use of enzyme-linked immunosorbent assay systems for serology and antigen detection in parvovirus, coronavirus and rotavirus infections in dogs in The Netherlands

Rimmelzwaan, Guus F.; Groen, Jan; Egberink, Herman; Borst, G. H. A.; Uytdehaag, Fons G. C. M.; Osterhaus, Albert D. M. E.

doi:10.1016/0378-1135(91)90039-i

Cited by 39 publications

(30 citation statements)

References 19 publications

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“…Both viruses were isolated from all feces samples collected. Earlier serological and virological studies had demonstrated that puppies and dogs from kennels and animal shelters are particularly exposed to these infections (Rimmelzwaan et al, 1991;Tennant et al, 1993;Bandai et al, 1999;Naylor et al, 2001;Yesilbag et al, 2004;Schulz et al, 2008). The high prevalence observed here reflects the high contagiosity of these two viruses.…”

Section: Discussionsupporting

confidence: 47%

Validation of a fecal scoring scale in puppies during the weaning period

Grellet

Feugier²,

Chastant-Maillard

et al. 2012

Preventive Veterinary Medicine

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In puppies weaning is a high risk period. Fecal changes are frequent and can be signs of infection by digestive pathogens (bacteria, viruses, parasites) and indicators of nutritional and environmental stress. The aim of this study was to define a pathological fecal score for weaning puppies, and to study the impact on that score of two intestinal viruses (canine parvovirus type 2 and canine coronavirus). For this, the quality of stools was evaluated on 154 puppies between 4 and 8 weeks of age (100 from small breeds and 54 from large breeds). The scoring was performed immediately after a spontaneous defecation based on a 13-point scale (from 1; liquid to 13; dry and hard feces). Fecal samples were frozen for further viral analysis. Each puppy was weighed once a week during the study period. The fecal score regarded as pathological was the highest score associated with a significant reduction in average daily gain (ADG). Fecal samples were checked by semi-quantitative PCR or RT-PCR for canine parvovirus type 2 and canine coronavirus identification, respectively. The quality of feces was affected by both age and breed size. In small breeds, the ADG was significantly reduced under a fecal score of 6 and 7 for puppies at 4-5 and 6-8 weeks of age, respectively. In large breeds, the ADG was significantly reduced under a fecal score of 5 whatever the age of the puppy. Whereas a high viral load of canine parvovirus type 2 significantly impacted feces quality, no effect was recorded for canine coronavirus. This study provides an objective threshold for evaluation of fecal quality in weaning puppies. It also emphasizes the importance to be given to age and breed size in that evaluation.

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Section: Discussionsupporting

confidence: 47%

Validation of a fecal scoring scale in puppies during the weaning period

Grellet

Feugier²,

Chastant-Maillard

et al. 2012

Preventive Veterinary Medicine

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“…Since then, several CCoV outbreaks have been reported worldwide, showing that CCoV is an important enteropathogen of the dog. Serological and virological investigations have demonstrated that CCoV is widespread in dog population, mainly in kennels and animal shelters (Carmichael, 1978;Rimmelzwaan et al, 1991;Tennant et al, 1993;Möstl et al, 1994;Bandai et al, 1999;Naylor et al, 2001b;Yeşilbag et al, 2004;Schulz et al, 2008). CCoV infection is characterised by high morbidity and low mortality, as well as by a typical faecal-oral route of transmission (Tennant et al, 1991).…”

Section: History and Pathobiologymentioning

confidence: 99%

An update on canine coronaviruses: Viral evolution and pathobiology

Decaro

Buonavoglia

2008

Veterinary Microbiology

161

142

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“…Assessment of antibodies by the virus neutralisation assay (Mochizuki et al, 1987), or by indirect enzyme-linked immunosorbent assay (ELISA) (Rimmelzwaan et al, 1991;Tuchiya et al, 1991) provides an indication of the exposure of an animal to CCoV. Detection of immunoglobulin M (IgM) and IgG against CCoV by indirect ELISA (Tennant et al, 1991;Naylor et al, 2001) determines current or previous exposure of an animal to CCoV.…”

Section: Introductionmentioning

confidence: 99%

Prevalence of canine coronavirus antibodies by an enzyme-linked immunosorbent assay in dogs in the south of Italy

Pratelli

Elia

Martella

et al. 2002

Journal of Virological Methods

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An enzyme-linked immunosorbent assay (Elisa), using as antigen canine coronavirus-infected CrFK cell supernatant, was developed to detect antibodies against canine coronavirus (CCoV). Out of a total of 109 dog serum samples, 80 which were positive by routine virus neutralisation test were also Elisa positive. Seventeen samples which were negative by the virus neutralisation test, were positive by Elisa and by the confirmatory Western blotting test. The Elisa was substantially more sensitive than the virus neutralisation test in detecting antibodies to CCoV and may be used as an alternative technique to virus neutralisation.

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The use of enzyme-linked immunosorbent assay systems for serology and antigen detection in parvovirus, coronavirus and rotavirus infections in dogs in The Netherlands

Cited by 39 publications

References 19 publications

Validation of a fecal scoring scale in puppies during the weaning period

Validation of a fecal scoring scale in puppies during the weaning period

An update on canine coronaviruses: Viral evolution and pathobiology

Prevalence of canine coronavirus antibodies by an enzyme-linked immunosorbent assay in dogs in the south of Italy

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