Evaluation of enzyme-linked immunosorbent assays and a haemagglutination inhibition tests for the detection of antibodies to Newcastle disease virus in village chickens using a Bayesian approach

Abstract

Newcastle disease (ND) is an endemic disease in village chickens in Ethiopia with substantial economic importance. The sensitivity (Se) and specificity (Sp) of a blocking enzyme-linked immunosorbent assay (bELISA, Svanova Biotech), indirect ELISA (iELISA, Laboratoire Service International) and haemagglutination inhibition (HI) test for ND virus (NDV) antibody detection were evaluated in a Bayesian framework in the absence of a gold standard test, on sera collected from unvaccinated chickens kept under the village production system in household flocks and at markets in two woredas (i.e. districts) of the Eastern Shewa zone, Ethiopia. The outcomes of the iElisa test differed dramatically from those of the two other tests with 92% of the samples testing positive as compared with less than 15% for b-Elisa and HI. iElisa results were also inconsistent with previous estimations of Newcastle serological prevalence. The information provided by the iElisa test was thus considered as highly unreliable, probably due to an extremely low specificity, and thus not considered in the

Rapid Newcastle Disease Virus Detection Based on Loop-Mediated Isothermal Amplification and Optomagnetic Readout

“…Newcastle disease (ND), together with highly pathogenic avian influenza, are regarded as the two most significant diseases of poultry as well as of other birds throughout the world . Caused by Newcastle disease virus (NDV), the ND outbreaks have flock mortality rates up to 100% in fully susceptible chickens, and can spread rapidly, resulting in severe economic losses. , While virus isolation in embryonated eggs remains the gold standard for NDV detection, molecular diagnostics, especially real-time reverse transcription PCR (real-time RT-PCR), are more commonly used because of their reliability. , However, real-time RT-PCR based methods require high-precision thermal cycling equipment, expensive optical detection of PCR amplification products (e.g., fluorescence readers), and complicated operation requiring specially trained personnel. This limits the potential for RT-PCR for rapid and cost-effective on-site epizootic monitoring especially in developing countries.…”

Immunosensor‐based rapid quantitative detection of Newcastle disease virus antibodies using innovative gold immunochromatographic assay

“…2007; Chaka et al . 2015). However, these methods are inconvenient and unsuitable for rapid on‐site detection of antibodies because they take several hours to complete and skilled operating personnel (Ngom et al .…”

Simple Label-Free Electrochemical Immunosensor in a Microchamber for Detecting Newcastle Disease Virus

“…Newcastle disease (ND) is one of the most contagious diseases in poultry that has widely spread in South East Asian countries including Vietnam and causes severe economic losses [1,2]. So far, the conventional qualitative methods such as hemagglutination inhibition (HI) [2], agar gel precipitation [3], and Latex agglutination tests [4] have been introduced for clinical diagnosis of ND. In addition, enzymelinked immunosorbent assay (ELISA) [2], polymerase chain reaction (PCR) [5,6], and immunofluorescence test [7] have also been used for the semiquantitative analysis.…”

“…So far, the conventional qualitative methods such as hemagglutination inhibition (HI) [2], agar gel precipitation [3], and Latex agglutination tests [4] have been introduced for clinical diagnosis of ND. In addition, enzymelinked immunosorbent assay (ELISA) [2], polymerase chain reaction (PCR) [5,6], and immunofluorescence test [7] have also been used for the semiquantitative analysis. Although these methods effectively determine NDV in infective samples, they require complicated procedures for sample preparations, sophisticated instruments for assays, and large periods of time for the completion of the assays.…”