Immunosensor‐based rapid quantitative detection of Newcastle disease virus antibodies using innovative gold immunochromatographic assay

Abstract

Aims A novel quantitative method for rapid Newcastle disease virus (NDV) antibody detection was developed based on an innovative gold immunochromatographic assay with a quantitative immunosensor. Methods and Results NDV antibody‐detecting test strips containing a two‐reaction system and double‐test lines (T1, T2) were prepared. The test results were judged according to the signal ratio between the test and control lines as measured by the quantitative immunosensor. The minimum detection limit of the test strips for NDV antibodies was 22 titres. In addition, the assay was highly specific because only NDV antibodies produced visible test lines on the strip. The clinical application of the strips was tested by detecting NDV antibodies in 506 serum samples collected from chickens. The results showed a coincidence of 92·49% with those of the haemagglutination inhibition assay. Conclusions The strips were successfully prepared and showed high specificity towards NDV, sensitivity and stability. Significance and Impact of the Study This study describes a new method for detection of NDV antibody and provides a reference basis for rapid and quantitative monitoring of NDV antibodies. This new method overcomes the limitation of the existing colloidal gold immunochromatography, which only produces qualitative or semi‐quantitative results.

Enhanced Detection of Major Pathogens and Toxins in Poultry and Livestock With Zoonotic Risks Using Nanomaterials-Based Diagnostics

“…Similar to avian influenza, ND can also be detected and diagnosed by using immunochromatographic assays. Recently, a quantitative antigen-based Au-immunochromatographic technique incorporated with viral protein antigens/AuNPs (haemagglutinin-neuraminidase) was developed, which allowed the detection of ND viral antibodies with a sensitivity of 2 2 titers without any cross-reactivity ( 42 ).…”

Meeting Contemporary Challenges: Development of Nanomaterials for Veterinary Medicine

“…Antibodies conjugated to NPs are more stable, biocompatible, and more sensitive to target antigens [ 12 ]. Effective bioanalytical kits of enzyme-linked immunosorbent assays (ELISAs) have been developed using nanomaterials to diagnose a number of animal infectious diseases, such as bird flu [ 13 , 14 , 15 , 16 ], post-weaning multisystem wasting syndrome (PMWS) caused by a porcine circovirus type 2 (PCV2) [ 17 , 18 ], Newcastle disease [ 19 , 20 , 21 ], and many others.…”

Review detection of Newcastle disease virus

“…The immune colloidal gold test strip has no cross-immune reaction with AIV, EDS, IB, and IBD which indicated that the test strips had high specificity and could specifically detect NDV antibodies. This method overcomes the limitation that the existing colloidal gold immunochromatographic method is only used for qualitative or semi-quantitative purposes ( 70 ). GICT technology is easy to operate and does not require other instruments.…”