Evaluation of eight enzyme immunoassays for detection of immunoglobulin G against Helicobacter pylori

Meijer, B. C.; Thijs, J. C.; Kleibeuker, JH; Zwet, A. A. Van; Berrelkamp, R. J. P.

doi:10.1128/jcm.35.1.292-294.1997

Cited by 51 publications

(16 citation statements)

References 16 publications

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“…The parameters of these assays vary with sensitivity ranging between 81 and 100% and specificity between 79 and 98%. 3,9,10 Previous studies have shown that the performance of diagnostic kits is often poor in geographically different regions, possibly because of the use of less relevant antigens. 4,11 In the present study, the whole-cell antigen extract from the H. pylori strain NCTC 11637 gave reasonable accuracy in an Australian population.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of anti‐Helicobacter pylori IgG2 antibody for the diagnosis of Helicobacter pylori infection in western and Chinese populations

Ren¹,

Borody

Pang

et al. 2004

Aliment Pharmacol Ther

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SUMMARYBackground: The performance of commercial Helicobacter pylori diagnostic kits developed for particular geographic regions has often been found to be of poor diagnostic value when applied to other regions, possibly because of infections being caused by different H. pylori strains in different regions. Aim: To evaluate the performance of an IgG2 anti-H. pylori enzyme-linked immunoassay test (Helirad Alert) for detection of H. pylori infection in both Australian and Hong Kong (Chinese) subjects.Methods: Serum samples were tested for H. pylori specific IgG2 and IgG antibodies by enzyme-linked immunoassay kits using identical antigen preparation in 168 Australian and 160 Hong Kong (Chinese) subjects diagnosed with dyspepsia.Results: Using a cut-off value determined by analysis of H. pylori-negative Australian samples, the sensitivity, specificity and accuracy of the IgG2 assay were 77.8, 97.4 and 91.1%, respectively, for the Australian samples and 96.3, 83.8 and 90% for Hong Kong samples. For the IgG assay, sensitivity, specificity and accuracy were 87.0, 99.1 and 95.2% for Australian samples and 97.5, 75 and 86.3% for Hong Kong samples respectively. Receiver-operating characteristic analysis showed better discrimination of H. pylori status when the IgG2 assay was applied to Hong Kong samples, while the IgG assay was better in the Australian samples.Conclusion: These data demonstrate that the Helirad Alert enzyme-linked immunoassay could provide a reliable method for screening H. pylori infection in both western and Chinese populations.

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Section: Discussionmentioning

confidence: 99%

Evaluation of anti‐Helicobacter pylori IgG2 antibody for the diagnosis of Helicobacter pylori infection in western and Chinese populations

Ren¹,

Borody

Pang

et al. 2004

Aliment Pharmacol Ther

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“…Patients' sera were screened for IgG antibodies against H. pylori using a standard enzyme-linked immunosorbent assay (ELISA) test (Pyloriset, Orion, Espoo, Finland) according to the manufacturer's instructions [27].…”

Section: Patients Biopsies and Seramentioning

confidence: 99%

Mucosal Production of Antigastric Autoantibodies in Helicobacter pylori Gastritis

Faller

Ruff²,

Reiche³

et al. 2000

Helicobacter

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“…In recent years, several diagnostic methods for detecting H. pylori infection have been developed and they can be divided into two clinical groups: (i) invasive methods, requiring endoscopy (i.e. histologic evaluation, culture, the rapid‐urease test or PCR performed on gastric biopsy specimens obtained at endoscopy) and (ii) non‐invasive tests, mainly urea breath tests and serologic measurement of anti‐ Helicobacter pylori (anti‐Hp) antibodies (4,5).…”

Section: Introductionmentioning

confidence: 99%

Detection and evaluation of salivary antibodies to Helicobacter pylori in dyspeptic patients

Sönmezoğlu

Baysal

Ergen

et al. 2005

International Journal of Clinical Practice

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Helicobacter pylori infection is one of the most prevalent infections in humans. The high prevalence and the association with peptic ulceration and gastric cancer require simple and non-invasive methods for the diagnosis of the infection. Detection of salivary anti-H. pylori IgG antibodies has advantages compared with those on serum. In this study, salivary immunoglobulin G response to H. pylori was evaluated in 100 consecutive dyspeptic patients by enzyme-linked immunosorbent assay (ELISA), in comparison with culture and histopathologic examination of gastric biopsy specimens obtained at endoscopic procedures and assessed the accuracy of salivary diagnosis of the infection. The overall sensitivity and specificity of the test were 87 and 73%, respectively. These results suggest that saliva testing for H. pylori antibodies could be used reliably for screening dyspeptic patients in general practice, especially in children in whom venesection is more difficult.

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Evaluation of eight enzyme immunoassays for detection of immunoglobulin G against Helicobacter pylori

Cited by 51 publications

References 16 publications

Evaluation of anti‐Helicobacter pylori IgG2 antibody for the diagnosis of Helicobacter pylori infection in western and Chinese populations

Evaluation of anti‐Helicobacter pylori IgG2 antibody for the diagnosis of Helicobacter pylori infection in western and Chinese populations

Mucosal Production of Antigastric Autoantibodies in Helicobacter pylori Gastritis

Detection and evaluation of salivary antibodies to Helicobacter pylori in dyspeptic patients

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