Agaricus bisporus mushrooms contain an abundance of ergosterol, which on exposure to UV irradiation is converted to vitamin D2. The present study evaluated the effects UV-C irradiation on vitamin D2 formation and its bioavailability in rats. Fresh button mushrooms were exposed to UV-C irradiation at mean intensities of 0.403, 0.316, and 0.256 mW/cm(2) from respective distances of 30, 40, and 50 cm for periods ranging from 2.5 to 60 min. Vitamin D2 and ergosterol were measured by HPLC-MS/MS. The stability and retention of vitamin D2 were assessed including the extent of discoloration during storage at 4 degrees C or at room temperature. Exposure to UV-C irradiation at 0.403 mW/cm(2) intensity from 30 cm distance resulted in a time-dependent increase in vitamin D2 concentrations that was significantly higher than those produced at intensities of 0.316 and 0.256 mW/cm(2) from distances of 40 and 50 cm, respectively. Furthermore, the concentrations of vitamin D2 produced after exposure to UV-C irradiation doses of 0.125 and 0.25 J/cm(2) for, 2.5, 5, and 10 min were 6.6, 15.6, and 23.1 microg/g solids, equivalent to 40.6, 95.4, and 141 microg/serving, respectively. The data showed a high rate of conversion from ergosterol to vitamin D2 at short treatment time, which is required by the mushroom industry. The stability of vitamin D2 remained unchanged during storage at 4 degrees C and at room temperature over 8 days (P = 0.36), indicating no degradation of vitamin D2. By visual assessment or using a chromometer, no significant discoloration of irradiated mushrooms, as measured by the degree of "whiteness", was observed when stored at 4 degrees C compared to that observed with mushrooms stored at room temperature over an 8 day period (P < 0.007). Vitamin D2 was well absorbed and metabolized as evidenced by the serum response of 25-hydroxyvitamin D in rats fed the irradiated mushrooms. Taken together, the data suggest that commercial production of button mushrooms enriched with vitamin D2 for improving consumer health may be practical.
Oropharyngeal candidiasis is associated with defects in cell-mediated immunity and is commonly seen in human immunodeficiency virus positive individuals and AIDS patients. A model for oral candidiasis in T-cell-deficient BALB/c and CBA/CaH nu/nu mice was established. After inoculation with 10 8 Candida albicans yeasts, these mice displayed increased levels of oral colonization compared to euthymic control mice and developed a chronic oropharyngeal infection. Histopathological examination of nu/nu oral tissues revealed extensive hyphae penetrating the epithelium, with polymorphonuclear leukocyte microabscess formation.
Adoptive transfer of either naive or immune lymphocytes into immunodeficient mice resulted in the recovery of these animals from the oral infection. Reconstitution of immunodeficient mice with naive CD4؉ but not CD8 ؉ T cells significantly decreased oral colonization compared to controls. Interleukin-12 and gamma interferon were detected in the draining lymph nodes of immunodeficient mice following reconstitution with naive lymphocytes. This study demonstrates the direct requirement for T lymphocytes in recovery from oral candidiasis and suggests that this is associated with the production of cytokines by CD4 ؉ T helper cells.
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