Evaluation of dynamic thiol-disulfide homeostasis and ischemia-modified albumin levels in patients with chronic lymphocytic leukemia

Abstract

This is the first study to evaluate both the dynamic thiol-disulfide homeostasis and ischemia-modified albumin (IMA) levels in patients with chronic lymphocytic leukemia (CLL). Twenty-nine patients with CLL and 20 controls were included in the study. The dynamic thiol-disulfide balance was determined by the newly developed colorimetric method by Erel. IMA levels were determined by the cobalt binding test. We found that total antioxidant status levels were lower while total oxidant status (TOS) and oxidative stress index (OSI) levels were significantly higher in patients with CLL than controls. Moreover, native and total thiol levels were found to be statistically significant between the study and control groups (p<0.001), whereas no statistically significant difference was noted for IMA levels (p=0.365). A negative correlation was observed between native and total thiol levels, leukocyte, lymphocyte, and TOS. Total bilirubin showed positive correlation with direct bilirubin and alkaline phosphatase. In addition, IMA levels showed a positive correlation with OSI. This study highlights measurement of native and total thiol and IMA levels in patients with CLL for the first time. Dynamic thiol-disulfide homeostasis may contribute in the pathophysiological mechanism, and follow-up to disease in patients with CLL.

Thiol/disulfide homeostasis as a new oxidative stress marker in patients with Fabry disease

“…Under OS, thiol groups transform into disulfide structures and these forms can be reduced back to thiol groups, thus providing dynamic thiol balance. 11 Simoncini et al 19 in their study with FD patients showed a significant impairment in OS status. They reported high levels of advanced oxidation protein products and reduced levels of antioxidant and thiols in FD compared to the healthy controls.…”

“…Under OS, thiol groups transform into disulfide structures and these forms can be reduced back to thiol groups, thus providing dynamic thiol balance. 11…”

“…7,8 Several studies in the literature have shown that OS plays a critical role in the pathogenesis of various diseases. [9][10][11][12][13][14][15][16][17][18] Studies have shown that FD patients have increased lipid and protein damage and decreased antioxidant defense system as a result of OS. [19][20][21] Increased intracellular Gb3 causes OS, which increases the expression of cellular adhesion molecules 1 in vascular endothelial cells.…”

“…22 Studies have shown the determination of dynamic thiol-disulfide balance as evidence of elevated OS. [9][10][11][12][13][14][15][16][17][18]23 This is the first study to show both dynamic thiol-disulfide balance and OS levels in FD patients.…”

Effect of mode of delivery on neonatal oxidative stress and dynamic thiol–disulfide homeostasis

“…This increased oxygen consumption leads to an increase in the production of free radicals, causing OS. 12,25,26 Thiols are composed of sulfur and hydrogen atoms attached to a carbon atom, and play a significant role in the elimination of OS in cells. 27 When exposed to oxidation, thiols transform into disulfide structures, which is an indication of an early cellular response to OS.…”