Evaluation of digital optical density of bone repair in rats medicated with ketoprofen

Abstract

The purpose of this study was to evaluate the influence of ketoprofen on bone repair process in tibiae of rats by means of analysis of the digital optical density. Twenty Wistar rats were assigned to two groups: an untreated control group and a group treated with ketoprofen. The experimental procedures comprised the following stages: general anesthesia, preparation of a unicortical bone defect on the left tibia of each rat, medication with ketoprofen and radiographic examination. Digital radiographic images were acquired using Visualix GX-S-HDI™ digital sensor and an x-ray equipment. Radiographs were taken at baseline, 7, 14, 21 and 30 days postoperatively and the optical density (OD) was evaluated using the Vix win TM 1.4 system. The mean values of OD readings were analyzed statistically by ANOVA and Tukey's test with significance level set at á=5%. The control group showed a statistically significant correlation (p=0.001) between time and optical density, while the ketoprofen group exhibited a weak and not statistically significant correlation (p=0.100). The control group presented the smallest OD ratios at days 1 and 7, and the greatest OD ratios at days 14, 21 and 30, with statistically significant difference (p=0.001). There was no significant differences (p=0.100) among the OD ratios in the ketoprofen group, regardless of the evaluation period. The findings of this study suggest that ketoprofen influenced bone repair process because there was an increase in optical density during the first week and delayed new bone formation after the 21st day.

Comparison of the effects of dexketoprofen trometamol, meloxicam and diclofenac sodium on fibular fracture healing, kidney and liver: An experimental rat model

“…Some animal studies have investigated the effects of ketoprofen, meloxicam, and diclofenac on bone healing, and some of these studies showed that these drugs impair the bone healing process, whereas others showed that these drugs did not affect the healing process [7,[39][40][41][42][43].…”

Short-term administration of non-selective and selective cox-2 nsaids do not interfere with bone repair in rats

“…Studying the action of ketoprofen in rats tibia repair, Martins et al (2005), noticed a negative influence of the drug on bone density when the administration exceeded 21 days. Possibly, this is one of the reasons why the obtained results from the present study did not agree with those found in literature.…”

Pain and ketoprofen: what is its role in clinical practice?

“…The healing process of fractures and bone defects is characterized by bone tissue regeneration; this phenomenon is due to the stimulation of specific growth factors that act on stromal cells arising from the inflammatory process, and in physiological conditions, may result in the formation of bone matrix. There is also evidence to suggest that ketoprofen does not seem to interfere with these processes in animal models (86,87). One study compared the effects of ketoprofen with a placebo control group in tibial defects in rats digitally analyzing the bone density up to 21 days after osteotomy (86).…”

“…There is also evidence to suggest that ketoprofen does not seem to interfere with these processes in animal models (86,87). One study compared the effects of ketoprofen with a placebo control group in tibial defects in rats digitally analyzing the bone density up to 21 days after osteotomy (86). In the control group, the optical density increased significantly in relation to time.…”