Evaluation of Different Carbon and Nitrogen Sources in Production of Rhamnolipids by a Strain of &lt;E1&gt;Pseudomonas aeruginosa

Santos, Alexandre Soares dos; Sampaio, Ana Paula W.; Vasquez, Gina S.; Anna, L. M. Santa; Pereira, Nei; Freire, Denise Maria Guimarães

doi:10.1385/abab:98-100:1-9:1025

Cited by 78 publications

(17 citation statements)

References 25 publications

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“…Similar results were reported by Santos [14] using the P. aeruginosa PA1 strain. In that study, an air bubbling system failed to control foam formation.…”

Section: Biosurfactant Productionsupporting

confidence: 91%

“…Between the hours 12 and 48, high nitrogen and phosphorus consumption was evident, which apparently influenced biomass production and biosurfactant synthesis. It has been reported that these nutrients stimulate microbial growth [14]. By contrast, an older study demonstrated that bacterial growth and surfactant accumulation were not modified by the addition of phosphate and magnesium [26].…”

Section: Kinetic and Modeling Of Rhamnose Production Cell Growth Anmentioning

confidence: 93%

“…Growth of the bacterial culture was performed on medium proposed by Santos et al [14], consisting of (g/l): NH 4 NO 3 1.7; Na 2 HPO 4 7.0; KH 2 PO 4 3.0; MgSO 4 .7H 2 O 0.2; yeast extract 5.0; and glucose 10.0.…”

Section: Media and Growth Conditionsmentioning

confidence: 99%

“…The process interruption, therefore, may be attributed to a limitation on the carbon needed for bacterial metabolism. Usually, the carbon to nitrogen ratio is important to the product yield of bioactive molecules [5,10,14,24,35]. The nitrogen source, in association with carbon availability, favors the maintenance of the necessary enzymatic machinery for the synthesis of rhamnose [10].…”

Section: Kinetic and Modeling Of Rhamnose Production Cell Growth Anmentioning

confidence: 99%

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Biosurfactant Production by Pseudomonas aeruginosa Grown in Residual Soybean Oil

Lima

Ribeiro

Sérvulo

et al. 2008

Appl Biochem Biotechnol

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Pseudomonas aeruginosa PACL strain, isolated from oil-contaminated soil taken from a lagoon, was used to investigate the efficiency and magnitude of biosurfactant production, using different waste frying soybean oils, by submerged fermentation in stirred tank reactors of 6 and 10 l capacities. A complete factorial experimental design was used, with the goal of optimizing the aeration rate (0.5, 1.0, and 1.5 vvm) and agitation speed (300, 550, and 800 rpm). Aeration was identified as the primary variable affecting the process, with a maximum rhamnose concentration occurring at an aeration rate of 0.5 vvm. At optimum levels, a maximum rhamnose concentration of 3.3 g/l, an emulsification index of 100%, and a minimum surface tension of 26.0 dynes/cm were achieved. Under these conditions, the biosurfactant production derived from using a mixture of waste frying soybean oil (WFSO) as a carbon source was compared to production when non-used soybean oil (NUSO), or waste soybean oils used to fry specific foods, were used. NUSO produced the highest level of rhamnolipids, although the waste soybean oils also resulted in biosurfactant production of 75-90% of the maximum value. Under ideal conditions, the kinetic behavior and the modeling of the rhamnose production, nutrient consumption, and cellular growth were established. The resulting model predicted data points that corresponded well to the empirical information.

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“…Similar results were reported by Santos [14] using the P. aeruginosa PA1 strain. In that study, an air bubbling system failed to control foam formation.…”

Section: Biosurfactant Productionsupporting

confidence: 91%

Section: Kinetic and Modeling Of Rhamnose Production Cell Growth Anmentioning

confidence: 93%

Section: Media and Growth Conditionsmentioning

confidence: 99%

Section: Kinetic and Modeling Of Rhamnose Production Cell Growth Anmentioning

confidence: 99%

See 2 more Smart Citations

Biosurfactant Production by Pseudomonas aeruginosa Grown in Residual Soybean Oil

Lima

Ribeiro

Sérvulo

et al. 2008

Appl Biochem Biotechnol

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show abstract

“…The growth medium (inoculum) contained (in g/L) glucose 10.0, BRY 11.0, NH 4 NO 3 5.7, MgSO 4 ·7H 2 O 0.2, Na 2 HPO 4 7.0, and KH 2 PO 4 3.0. The medium containing the inoculum was adjusted to pH 7 and sterilized at 121°C for 15 Min 11,12.…”

Section: Methodsmentioning

confidence: 99%

Use of a greasy effluent floater treatment station from the slaughterhouse for biosurfactant production

Borges

Cardoso

Resende

2012

Biotech and App Biochem

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Most commercially available surfactants are produced from petroleum. However, increasing concerns about the environment have stimulated the search for biosurfactant production. This work examines biosurfactant production from the greasy effluent floater treatment station from the slaughterhouse of poultry and pigs. The biosurfactant production was evaluated using two strains of Pseudomonas aeruginosa [American Type Culture Collection (ATCC) 9027 and 10145] in a kinetic study to determine which strain produces a higher rhamnolipid concentration, which is characterized by the rhamnose concentration. The strain of P. aeruginosa was selected via a central composite design based on the following variables: fat concentration, nitrogen concentration, added ammonium nitrate (AN), and brewery residual yeast (BRY). The preliminary results show that the P. aeruginosa strain ATCC 10145 produced biosurfactant more efficiently than ATCC 9027. After optimizing the variables that were studied, the best fat, BRY, and AN concentrations (in g/L) were found to be 12, 15, and 0, respectively.

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Comparison of mono‐rhamnolipids and di‐rhamnolipids on microbial enhanced oil recovery (MEOR) applications

Rocha

Castilho

Castro

et al. 2020

Biotechnology Progress

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Rhamnolipids (RMLs) have more effectiveness for specific uses according to their homologue proportions. Thus, the novelty of this work was to compare mono‐RMLs and di‐RMLs physicochemical properties on microbial enhanced oil recovery (MEOR) applications. For this, RML produced by three strains of Pseudomonas aeruginosa containing different homologues proportion were used: a mainly mono‐RMLs producer (mono‐RMLs); a mainly di‐RMLs producer (di‐RMLs), and the other one that produces relatively balanced amounts of mono‐RML and di‐RML homologues (mono/di‐RML). For mono‐RML, the most abundant molecules were Rha‐C10C10 (m/z 503.3), for di‐RML were RhaRha‐C10C10 (m/z 649.4) and for Mono/di‐RML were Rha‐C10C10 (m/z 503.3) and RhaRha‐C10C10 (m/z 649.4). All RMLs types presented robustness under high temperature and variation of salinity and pH, and high ability for oil displacement, foam stability, wettability reversal and were classified as safe for environment according to the European Union Directive No. 67/548/EEC. For all these properties, it was observed a highlight for mono‐RML. Mono‐RML presented the lowest surface tension (26.40 mN/m), interfacial tension (1.14 mN/m), and critical micellar concentration (CMC 27.04 mg/L), the highest emulsification index (EI24 100%) and the best wettability reversal (100% with 25 ppm). In addition, mono‐RML showed the best acute toxicity value (454 mg/L), making its application potential even more attractive. Based on the results, it was concluded that all RMLs homologues studied have potential for MEOR applications. However, results showed that mono‐RML stood out and have the best mechanism of oil incorporation in micelles due their most effective surface‐active physicochemical features.

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Evaluation of Different Carbon and Nitrogen Sources in Production of Rhamnolipids by a Strain of <E1>Pseudomonas aeruginosa

Cited by 78 publications

References 25 publications

Biosurfactant Production by Pseudomonas aeruginosa Grown in Residual Soybean Oil

Biosurfactant Production by Pseudomonas aeruginosa Grown in Residual Soybean Oil

Use of a greasy effluent floater treatment station from the slaughterhouse for biosurfactant production

Comparison of mono‐rhamnolipids and di‐rhamnolipids on microbial enhanced oil recovery (MEOR) applications

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