Use of a greasy effluent floater treatment station from the slaughterhouse for biosurfactant production

Borges, Wesley da Silva; Cardoso, Vicelma Luiz; Resende, Miriam Maria de

doi:10.1002/bab.1018

Cited by 10 publications

(3 citation statements)

References 27 publications

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“…Slaughterhouse material was previously proven to allow for laboratory cultivation of different strains with proteolytic, lipolytic, hemolytic, and biosurfactant activities3132333435. Here, we expected that a microbial community residing in a habitat rich in blood, fats, remnants of animal skin and flesh would be adapted to the efficient degradation of proteins and lipids.…”

Section: Resultsmentioning

confidence: 99%

“…A microbial community living in a biofilm attached to a solid surface of a blood bottom drain in the butchery Frenken Vieh- und Fleisch GmbH (Düren, Germany) was used as eDNA source for the construction of metagenomic libraries. Slaughterhouse material was previously proven to allow for laboratory cultivation of different strains with proteolytic, lipolytic, hemolytic, and biosurfactant activities 31 32 33 34 35 . Here, we expected that a microbial community residing in a habitat rich in blood, fats, remnants of animal skin and flesh would be adapted to the efficient degradation of proteins and lipids.…”

Section: Resultsmentioning

confidence: 99%

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Metagenomic discovery of novel enzymes and biosurfactants in a slaughterhouse biofilm microbial community

Thies

Rausch

Kovačić

et al. 2016

Sci Rep

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DNA derived from environmental samples is a rich source of novel bioactive molecules. The choice of the habitat to be sampled predefines the properties of the biomolecules to be discovered due to the physiological adaptation of the microbial community to the prevailing environmental conditions. We have constructed a metagenomic library in Escherichia coli DH10b with environmental DNA (eDNA) isolated from the microbial community of a slaughterhouse drain biofilm consisting mainly of species from the family Flavobacteriaceae. By functional screening of this library we have identified several lipases, proteases and two clones (SA343 and SA354) with biosurfactant and hemolytic activities. Sequence analysis of the respective eDNA fragments and subsequent structure homology modelling identified genes encoding putative N-acyl amino acid synthases with a unique two-domain organisation. The produced biosurfactants were identified by NMR spectroscopy as N-acyltyrosines with N-myristoyltyrosine as the predominant species. Critical micelle concentration and reduction of surface tension were similar to those of chemically synthesised N-myristoyltyrosine. Furthermore, we showed that the newly isolated N-acyltyrosines exhibit antibiotic activity against various bacteria. This is the first report describing the successful application of functional high-throughput screening assays for the identification of biosurfactant producing clones within a metagenomic library.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Metagenomic discovery of novel enzymes and biosurfactants in a slaughterhouse biofilm microbial community

Thies

Rausch

Kovačić

et al. 2016

Sci Rep

View full text Add to dashboard Cite

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“…The concentrations (in g/L) selected for the fat, ammonium nitrate and residual brewery yeast were 12, 0 and 15, respectively, according to Borges et al (2012).…”

Section: Culture Medium For Maintenance Of Strains and Growth Conditionsmentioning

confidence: 99%

Optimization of the Operating Conditions for Rhamnolipid Production Using Slaughterhouse-Generated Industrial Float as Substrate

Borges

Moura

Filho

et al. 2015

Braz. J. Chem. Eng.

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-Biosurfactants have a wide range of applications in emulsions, separations, and solubilization because these chemicals reduce the surface tension and viscosity of solutions. This work studied rhamnolipid production using a batch bioreactor with a working volume of 1.5 liters, Pseudomonas aeruginosa ATCC (American Type Culture Collection) 10145 strain, and the greasy effluent from the slaughter of poultry and pigs as the substrate. The main goal of this research was to evaluate the level of aeration, agitation speed and inoculum concentration using a Central Composite Design (CCD). Experimental conditions were selected using the surface response technique obtained from the CCD, and the results were validated to test the reproducibility. The following operating conditions were selected: 1.2 vvm level of aeration, 600 rpm agitation speed, and 1.0 g/L biomass inoculum concentration. Under these conditions, the following results were obtained: the rhamnose production, surface tension and emulsifying index were 5.37 g/L, 25.6 dyne/cm and 100%, respectively.

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In situ downstream strategies for cost‐effective bio/surfactant recovery

Najmi

Ebrahimipour

Franzetti

et al. 2018

Biotech and App Biochem

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Since 60-80% of total costs of production are usually associated with downstream collection, separation, and purification processes, it has become advantageous to investigate how to replace traditional methods with efficient and cost-effective alternative techniques for recovery and purification of biosurfactants. In the traditional techniques, large volumes of organic solvents are usually used for increasing production cost and the overall environmental burden. In addition, traditional production and separation methods typically carried out in batch cultures reduce biosurfactant yields due to product inhibition and lower biosurfactants activity as a result of interaction with the organic solvents used. However, some in situ recovery methods that allow continuous separation of bioproducts from culture broth leading to an improvement in yield production and fermentation efficiency. For biosurfactants commercialization, enhancement of product capacity of the separation methods and the rate of product removal is critical. Recently, interest in the integration of separation methods with a production step as rapid and efficient techniques has been increasing. This review focuses on the technology gains and potentials for the most common methods used in in situ product removal: foam fractionation and ultrafiltration, especially used to recover and purify two well-known biosurfactants: glycolipids (rhamnolipids) and lipopeptides (surfactins).

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Use of a greasy effluent floater treatment station from the slaughterhouse for biosurfactant production

Cited by 10 publications

References 27 publications

Metagenomic discovery of novel enzymes and biosurfactants in a slaughterhouse biofilm microbial community

Metagenomic discovery of novel enzymes and biosurfactants in a slaughterhouse biofilm microbial community

Optimization of the Operating Conditions for Rhamnolipid Production Using Slaughterhouse-Generated Industrial Float as Substrate

In situ downstream strategies for cost‐effective bio/surfactant recovery

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