Evaluation of cytotoxicity and genotoxicity of some Philippine medicinal plants

Abstract

The genotoxicity and toxicity of ethnomedicinal Philippine plants, which include Cassia fistula, Derris elliptica, Ficus elastica, Gliciridia sepium, Michelia alba, Morus alba, Pogostemon cablin and Ricinus communis, were tested using the Vitotox assay. The plants are used traditionally to treat several disorders like diabetes, weakness, menorrhagia, headache, toothache and rheumatism. The dried leaves were homogenized for overnight soaking in methanol at room temperature. The resulting alcoholic extracts were filtered and concentrated in vacuo and tested for their genotoxicity and cytotoxicity using Vitotox®. Results showed that the medicinal plants that were tested are not genotoxic nor cytotoxic, except for R. communis and P. cablin, which showed toxicity at high doses (low dilutions) in the absence of S9.

Short peptide analogs as alternatives to collagen in pro-regenerative corneal implants

“…In vitro cytotoxicity and genotoxicity assessments were performed using the Vitotox™ protocol (Gentaur, Belgium), a highly sensitive technique that is closely related to the Ames test. In this test, two different strains of Salmonella typhimurium used respectively for cytotoxicity and genotoxicity testing were exposed to different dilutions of homogenized hydrogels, along with positive controls [25] . Neither the RHCIII-MPC controls ( Fig.…”

Antidiabetic Potential of Medicinal Plants and Their Active Components

“…Traditional knowledge of antidiabetic Asian plants: (1) Review in Iran [50,51,52,53,54]; (2) Review in Jordan [55,56,57]; (3)Review in Malaysia [58,59]; (4) Review in Mongolia [60]; (5) Review in Philippines [61,62]; (6) Review in Saudi Arabia [63,64,65]; (7) Review in Korea [66,67,68]; (8) Review in Sri Lanka [69]; (9) Review in Syria [70]; (10) Review in Thailand [71,72,73,74,75]; (11) Review in Turkey [76,77,78,79,80,81,82]; (12) Review in Vietnam [83,84,85]; (13) Review in India [86,87,88,89,90,91,92,93,94,95,96,97,98,99,100,101,102,103,104,105]; and (14) Review in China [99,106,107,…”

“…The leaves and edible fruits of Ficus hispida are used for the treatment of diabetes [116] and is used in Ayurveda [117], Siddha [118], and traditional African medicine [119]. Ficus elastica , an ethnomedicinal Filipino plant, exhibits less toxicity [62], which is used in diabetes treatment.…”

A 28-Day Repeated Dose Toxicological Study of an Aqueous Extract of Morus Alba L.

“…Numerous other published studies on various M alba leaf extracts, including genotoxicity, mutagenicity, and acute oral toxicity studies, as well as a subchronic repeated dose oral toxicity study on silkworm powder extract (containing 1.25% DNJ) have also demonstrated a general lack of safety concerns at doses of up to 5,000 mg/kg bw/d in rodents. 23 –31…”