Evaluation of Chlorhexidine Toxicity Injected in the Paw of Mice and Added to Cultured L929 Fibroblasts

Faria, Gisele; Celes, Mara Rúbia Nunes; Rossi, Andiara De; Silva, Lea Assed B.; Silva, João; Rossi, Marcos A.

doi:10.1016/j.joen.2006.12.023

Cited by 77 publications

(77 citation statements)

References 36 publications

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“…The results indicated that leukocytes and kidney cells are potential targets for primary DNA damage after oral exposure to CHX. Faria et al (26) have reported that CHX injected in the subplantar space of the hind paw of mice induced severe toxic effects, as evidenced by necrotic changes in the epidermis, dermis, and subcutaneous tissue in association with reactive inflammatory response, particularly at higher concentrations. In addition, in cultured fibroblasts, CHX induced apoptosis at lower concentrations and necrosis at higher concentrations and increased expression of heatshock protein 70, an indicator of cellular stress.…”

Section: Discussionmentioning

confidence: 99%

Determination of para-Chloroaniline and Reactive Oxygen Species in Chlorhexidine and Chlorhexidine Associated with Calcium Hydroxide

Barbin

Saquy

Guedes

et al. 2008

Journal of Endodontics

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Section: Discussionmentioning

confidence: 99%

Determination of para-Chloroaniline and Reactive Oxygen Species in Chlorhexidine and Chlorhexidine Associated with Calcium Hydroxide

Barbin

Saquy

Guedes

et al. 2008

Journal of Endodontics

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“…One milliliter of culture medium of each material and dilution combination was then added to 10 wells in the tissue culture plate (n = 10 per combination). As a negative control, cells were cultured in medium only, and as a positive control, cells were cultured in medium containing 0.010% chlorhexidine (11,19).…”

Section: Cytotoxicitymentioning

confidence: 99%

Cytotoxicity Comparison of Three Current Direct Pulp-capping Agents with a New Bioceramic Root Repair Putty

Hirschman

Wheater

Bringas

et al. 2012

Journal of Endodontics

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“…A strong anti-inflammatory effect was associated to CHX, by reducing the activity of matrix metalloproteinases (MMPs) (7), the basal concentration of leucocytes and the levels of the proinflammatory cytokines (11). However, it has been related toxicity effects from CHX (12)(13)(14)(15), even at a very low concentration (16). This drug has been demonstrated to induce an inflammatory reaction (14), tissue necrosis (15) and to retard granulation tissue formation and wound healing (13).…”

Section: Introductionmentioning

confidence: 99%

“…However, it has been related toxicity effects from CHX (12)(13)(14)(15), even at a very low concentration (16). This drug has been demonstrated to induce an inflammatory reaction (14), tissue necrosis (15) and to retard granulation tissue formation and wound healing (13). Besides, CHX behaved in a cytotoxic manner toward osteoblasts in vitro (17).…”

Section: Introductionmentioning

confidence: 99%

Biocompatibility of a chlorhexidine local delivery system in a subcutaneous mouse model

Monteiro¹,

Macedo²,

Macedo³

et al. 2011

Med Oral

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Objective: This study aimed evaluating histologically and histomorphometrically the response of the conjunctive tissue face to the implant of chlorhexidine chips in the subcutaneous tissues of rats. Study Design: In this research 35 male rats Wistar were used to analyze the biocompatibility and the degradation process of chlorhexidine chip. In each animal, it was made 2 incisions for subcutaneous implantation of chlorhexidine chip (test group) and a polytetrafluorethylene membrane (control group). The morphological changes in subcutaneous implantations were assessed after 1, 3, 5, 7, 10, 14, 21 days. The data were submitted to Friedman nonparametric test to analyze the comparisons among observation periods and to allow the comparison among groups. Results: Differences were found in the analysis of the inflammatory response when comparing the tested materials (p values ≤ 0.05). In test group was observed hemorrhage, edema and intense inflammatory infiltrate predominantly neutrophilic around material. From 3-day and subsequent periods was verified granulation tissue externally at this infiltrate. From 10-day on was observed crescent area of degradation of chlorhexidine chip, associated with neutrophilic and macrophagic infiltrate, that maintained until 21-day. In the control group, moderate inflammatory infiltrate was observed initially, predominantly polymorphonuclear, edema and granulation tissue 3-day period. The inflammatory infiltrate was gradually replaced for granulation tissue, culminating in a fibrous capsule. Giant multinucleate cells situated at contact interface with the coating was examined since 3-day and persisted until 21-day. Conclusion: The chlorhexidine chip induces an intense acute inflammatory response at subcutaneous tissue of rats. Therefore, at conditions of this study was not biocompatible.

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Evaluation of Chlorhexidine Toxicity Injected in the Paw of Mice and Added to Cultured L929 Fibroblasts

Cited by 77 publications

References 36 publications

Determination of para-Chloroaniline and Reactive Oxygen Species in Chlorhexidine and Chlorhexidine Associated with Calcium Hydroxide

Determination of para-Chloroaniline and Reactive Oxygen Species in Chlorhexidine and Chlorhexidine Associated with Calcium Hydroxide

Cytotoxicity Comparison of Three Current Direct Pulp-capping Agents with a New Bioceramic Root Repair Putty

Biocompatibility of a chlorhexidine local delivery system in a subcutaneous mouse model

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