Cytotoxicity Comparison of Three Current Direct Pulp-capping Agents with a New Bioceramic Root Repair Putty

Hirschman, Wade R.; Wheater, Michelle; Bringas, Josef S.; Hoen, Michel

doi:10.1016/j.joen.2011.11.012

Cited by 90 publications

(75 citation statements)

References 16 publications

(24 reference statements)

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“…At the same time, OH − was released and started to cause cytotoxicity 19) . An in vitro study indicated that Ultra-Blend Plus had no increase of adult human dermal fibroblast cytotoxicity levels, compared to negative control 20) . It is worth to note that a typical 70% of polymerization degree of conversion of dimethacrylate monomers does not mean 30% of free monomers.…”

Section: Calcium Hydroxide and Resin-modified Calcium Hydroxidementioning

confidence: 98%

“…Another study also suggested that calcium silicates (ProRoot MTA, MTA-Angelus (Angelus, Londrina, PR, Brazil), Biodentine) were less cytotoxic to odontoblasts cells than two-paste calcium hydroxide (Dycal) and one-paste calcium hydroxide (Calcicur) 8) . An in vitro adult human dermal fibroblast cytotoxicity study showed that MTA materials (MTA-Angelus, Brasseler Endosequence Root Repair Putty) and resin-modified Ca(OH) 2 (Ultra-Blend Plus) had cytotoxicity levels statistically similar to the negative control, which was statistically less cytotoxic than the two-paste calcium hydroxide (Dycal) 20) . MTA also showed less toxic to human tooth germ stem cells than Dycal 26) .…”

Section: Calcium Silicates and Resin-modified Calcium Silicatesmentioning

confidence: 99%

See 1 more Smart Citation

Cytotoxicity and biocompatibility of resin-free and resin-modified direct pulp capping materials: A state-of-the-art review

Chen¹,

Suh²

2017

Dental Materials Journal

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Direct pulp capping is the placement of a dental material directly over exposed pulp to prevent dental pulp from dying and avoid the need for root canal treatments. Calcium hydroxide and calcium silicates/mineral trioxide aggregates (MTA) have been commonly used for direct pulp capping with great success clinically. In recent years, resin-modified calcium hydroxide and calcium silicates have been developed with the advantages of precise placement, command set, and superior physical strength. As pulp capping materials would be in direct contact with the pulp, the cytotoxicity and biocompatibility is of particular importance in order to avoid pulp irritation and maintain pulp vitality. Therefore, this review article will summarize the cytotoxicity and biocompatibility of direct pulp capping materials, particularly resin-modified materials.

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Section: Calcium Hydroxide and Resin-modified Calcium Hydroxidementioning

confidence: 98%

Section: Calcium Silicates and Resin-modified Calcium Silicatesmentioning

confidence: 99%

Cytotoxicity and biocompatibility of resin-free and resin-modified direct pulp capping materials: A state-of-the-art review

Chen¹,

Suh²

2017

Dental Materials Journal

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“…Recent studies showed that the cytotoxic effects imposed by Ortho MTA, [26,27] Retro MTA, [37] BioAggregate, [18,28,29] Biodentine, [26,28,38,39] MTA Plus [40] and MTA Angelus exhibited negligible cytotoxicity. [41,42] While the extracts of BioAggregate promoted cell viability, Biodentine showed a reduced number of cells. The overall effects of BioAggregate seemed to be better than those of MTA and Biodentine, and to indicate a better cytocompatibility pattern.…”

Section: Microscopy Resultsmentioning

confidence: 99%

Evaluation of cytotoxicity and gelatinases activity in 3T3 fibroblast cell by root repair materials

Varol

Bahar

Karsli

et al. 2016

Biotechnology & Biotechnological Equipment

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The aim of this study was to investigate the effects of calcium silicate-based products on cytotoxicity in the 3T3 fibroblast and gelatinolytic activity of matrix metalloproteinases (MMPs). 3T3 fibroblasts were incubated directly with Ortho Mineral trioxide aggregate (MTA), BioAggregate, Biodentine, MTA Plus, MTA Angelus and MTA Cerkamed for 24 hours and seven days. The cytotoxicity was determined using an MTT assay. Supernatants were collected to determine MMP-2 and MMP-9. Data were analysed using IBM SPSS 22. Seventh day extracts of Ortho MTA and Biodentine showed reduced cell viability. Specific characterization of MMPs in cell culture demonstrated that MMP-2 (62 kPa) in the cell culture supernatants by gelatin zymography showed induced expression in four out of seven groups by 3T3 cells. No MMP-9 expression was observed. The cytotoxicity of materials revealed a significant difference in cell viability between the groups on the first and seventh days. The results of this study revealed minor cytotoxic effects for Ortho MTA and Biodentine. This study suggests that endodontic sealers induced production of MMP-2. MMP-9 might be expressed in small amounts when compared with MMP-2.

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“…Some studies (CHANG et al, 1998;CAMARGO et al, 2009;SEPET et al, 2009;SILVA et al, 2012A;SILVA et al, 2012B;YOSHINO et al, 2013) used fibroblasts for quick and easy growth (HIRSHMAN et al, 2012), in addition to their availability in periapical tissues and the periodontal ligament, which are areas that are susceptible to the effects of intracanal medications and their degradation products (YOSHINO et al, 2013). Fibroblasts are also the largest producers of collagen tissue and, therefore, actively participate in the repair tissue process (SILVA et al, 2012B).…”

Section: Discussionmentioning

confidence: 99%

Assessment of intracanal medications cytotoxicity on L929 fibroblast cells

Farias¹,

Matos²,

Carvalho³

et al. 2016

Biosci. J.

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ABSTRACT:This study aimed to evaluate the cytotoxicity of intracanal medications on L929 fibroblast cells at different periods of observation. The following experimental groups were studied: calcium hydroxide with camphorated paramonochlorophenol and glycerin (CPG); iodoform with glycerin (IG); calcium hydroxide with iodoform and distilled water (CIW); iodoform with distilled water (IW); calcium hydroxide with distilled water (CW); Otosporin ® (OT); and a control group composed of cells and culture medium. Eluates were prepared from each group and placed in contact with 1 x 10 5 cells/well for periods of 30 minutes, 12, 24, 48 and 72 hours, 5 and 7 days. After each experimental period, a cytotoxicity test was performed using methyltetrazolium (MTT) and a spectrophotometer at an optical density of 570 nm to analyze cell viability. The ANOVA and Tukey test with a significance level of 5% was used to analyze the data. At 30 minutes and at 12 hours, all groups were equal to the control group. At 24 hours, there was greater cytotoxicity in the IG group than in the control group (P<0.001). At 48 hours, only the OT group was cytotoxic (P <0.001). At 72 hours and at 5 days, the most cytotoxic groups were CW and OT. At 7 days, the IW and CPG groups were the least cytotoxic (P <0.001). With respect to experimental time, significant differences between 24 hours and 7 days were observed in all groups. Otosporin ® was the most cytotoxic medication, followed by calcium hydroxide with distilled water.

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Cytotoxicity Comparison of Three Current Direct Pulp-capping Agents with a New Bioceramic Root Repair Putty

Cited by 90 publications

References 16 publications

Cytotoxicity and biocompatibility of resin-free and resin-modified direct pulp capping materials: A state-of-the-art review

Cytotoxicity and biocompatibility of resin-free and resin-modified direct pulp capping materials: A state-of-the-art review

Evaluation of cytotoxicity and gelatinases activity in 3T3 fibroblast cell by root repair materials

Assessment of intracanal medications cytotoxicity on L929 fibroblast cells

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