Evaluation of Chemicals with Endocrine Modulating Activity in a Yeast-Based Steroid Hormone Receptor Gene Transcription Assay

Gaido, Kevin W.; Leonard, Linda S.; Lovell, Stephanie; Gould, Janet C.; Babaı̈, Dariouch; Portier, Christopher J.; McDonnell, Donald P.

doi:10.1006/taap.1996.8069

Cited by 634 publications

(367 citation statements)

References 48 publications

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“…200 L of the supernatant was transferred into a new 96-well plate, and the OD 420nm was determined. The ␤-galactosidase activity was calculated according to equations described by Gaido et al [22].…”

Section: The Conventional Yeast Assaymentioning

confidence: 99%

Thyroid hormone disrupting activities of sediment from the Guanting Reservoir, Beijing, China

Ren

et al. 2014

Journal of Hazardous Materials

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h i g h l i g h t s• Yeast bioassays were used to evaluate the TR disrupting activities of the sediments.• The organic extracts showed TR antagonistic activities.• The elutriates also significantly antagonized the TR.• The causes of toxicity in the elutriates appear to be partly related to the heavy metals. a r t i c l e i n f o a b s t r a c tIn the present study, yeast bioassays were used to evaluate and characterize the thyroid receptor (TR) disrupting activities of the organic extracts and elutriates of the sediments from the Guanting Reservoir, Beijing, China. An accelerated solvent extraction was used to separate the organic extracts, which were subjected to a yeast bioassay. The organic extracts could affect thyroid hormone signaling by decreasing the binding of the thyroid hormone. The TR antagonistic activity equivalents (TEQ bio ) referring to amiodarone hydrochloride were calculated and the observed TEQ bio-organic extracts ranged from 25.4 ± 3.7 to 176.9 ± 18.0 g/g. Elutriate toxicity tests using the modified yeast bioassay revealed that the elutriates also significantly antagonized the TR, with the TEQ bio-elutriates ranging from N.D. to 7.8 ± 0.8 g/L. To characterize the toxic compounds, elutriates were extracted by using a C18 cartridge or treated with ethylenediaminetetraacetic acid (EDTA, 30 mg/L). The results suggested that the addition of EDTA eliminated over 74.3% of the total effects, and the chemical analysis revealed that heavy metals, some of which exhibited TR disrupting potency, for example Zn and Cd, were detectable with higher concentrations in the elutriates. Thus, the cause(s) of toxicity in the elutriate appear to be partly related to the heavy metals.

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Section: The Conventional Yeast Assaymentioning

confidence: 99%

Thyroid hormone disrupting activities of sediment from the Guanting Reservoir, Beijing, China

Ren

et al. 2014

Journal of Hazardous Materials

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“…The bioassay, particularly the in vitro assay, could be an effective supplementary method for the primary screening of thyroid-disrupting activities due to its ease of manipulation and ability to process large number of samples quickly and inexpensively (Murk et al 2013;Shi et al 2011;Gaido et al 1997). Unfortunately, these bioassays often require water-sample pretreatment, which not only remove interference from the matrix but also eliminates some TDCs in the tested water samples.…”

Section: Discussionmentioning

confidence: 99%

“…The reactions were terminated by adding 100 lL of 1 mol/L Na 2 CO 3 ; 200 lL of the supernatant was transferred into a new 96-well plate, and the OD 420nm was determined. b-galactosidase activity was calculated according to equations described by Gaido et al (1997).…”

Section: Sample Collection and Processingmentioning

confidence: 99%

Identification of Thyroid-Receptor Antagonists in Water from the Guanting Reservoir, Beijing, China

Ren

Han

et al. 2014

Arch Environ Contam Toxicol

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Thyroid hormone (TH) has long been known to be essential for normal brain development in both humans and animals, and increasing evidence suggests that environmental components may disrupt TH signaling. In the present study, two-hybrid yeast bioassay and chemical analysis were used to evaluate and identify thyroid-receptor (TR) disruptors in water from the Guanting Reservoir, Beijing, China. Modified yeast bioassay showed that the water samples could affect TH signaling. The bioassay-derived amiodarone hydrochloride equivalents ranged from 33.8 ± 3.3 to 308.5 ± 31.8 lg/L. Solid-phase extraction was used to separate the organic extracts, which were subjected to bioassay and chemical analysis. The organic extracts significantly antagonized the TR, which accounted for [86.0 % of the total effects. Thus, organic extracts may play a major role in the TR-disruption activity of the water. Phenols, organochlorine pesticides, and phthalate esters were detected in the organic extracts. Chemical analysis and toxic-equivalent calculation showed that a major cause of the TR antagonism of the water was dibutyl phthalate (80.1 to 122.7 %).

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“…To ensure that activity in the bioassay was caused by true antagonistic responses not by cytotoxicity, the result was excluded if OD 600 value was less than 10% of that of the negative control. The ␤-galactosidase activity was calculated according to equations described previously by Gaido et al [33].…”

Section: Yeast Assaymentioning

confidence: 99%

Chlorination by-products of bisphenol A enhanced retinoid X receptor disrupting effects

Jiang²,

et al. 2016

Journal of Hazardous Materials

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Evaluation of Chemicals with Endocrine Modulating Activity in a Yeast-Based Steroid Hormone Receptor Gene Transcription Assay

Cited by 634 publications

References 48 publications

Thyroid hormone disrupting activities of sediment from the Guanting Reservoir, Beijing, China

Thyroid hormone disrupting activities of sediment from the Guanting Reservoir, Beijing, China

Identification of Thyroid-Receptor Antagonists in Water from the Guanting Reservoir, Beijing, China

Chlorination by-products of bisphenol A enhanced retinoid X receptor disrupting effects

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