Evaluation of carbon sources, gelling agents, growth hormones and additives for efficient callus induction and plant regeneration in Indian wheat (Triticum aestivum L.) genotypes using mature embryos

Malik, Kamla; Birla, Deepshikha; Yadav, Honey; Sainger, Manish; Chaudhary, Darshna; Jaiwal, Pawan K.

doi:10.1007/s12892-017-0046-0

Cited by 12 publications

(5 citation statements)

References 38 publications

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“…Studies were also extended to investigate a wide range of plant species and crops in an attempt to screen the best ingredients to be used in explants and medium components. Our study has also produced convincing results consistent with those obtained with bananas (Kacar et al 2010), wheat (Malik et al 2017), kiwi (Mardiana et al 2018), oil and date palms (Al-Mayahi and Ali 2021; Palanyandy et al 2020), and rice (Gehad M. Mohamed et al 2021;Repalli et al 2019).…”

Section: Mechanical Strengthsupporting

confidence: 88%

Developed applicability of a bacterial cellulose matrix as a gelling substitute for plant tissue culture media

et al. 2022

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Bacterial cellulose (BC) is a natural biodegradable, eco-friendly fiber, lying within the nanoscale range. It is reputable for its various physical and chemical qualities, like high hydrophilicity, immense crystallinity, ease of sterility, being toxin-free, and extremely pure. Adding to its wide applicability in different fields, this study evaluated the applicability of a developed gelling substitute for plant tissue culture media. The BC matrix was characterized under the acronym PLATIBACGEL (PLAnt TIssue Culture BActerial Cellulose GEL), formed by Komagataeibacter hansenii AS.5, preisolated from rotten apple waste. Scanning electron microscope, Fourier-transform infrared, X-ray diffractometer, and tensile strength analyses confirmed the formation of purified, porous, and heterogeneous densely packed multiple network polymers possessing cellulose properties. The water holding capacity (WHC) values of wet and dried BC membranes were 9179% and 226.9%, respectively, and the water absorption rate (WAR) of dry BC membranes was higher than that of wet membranes. Using BC as a tissue culture gelling agent, six genotypes from tomato and wheat seeds were cultured in vitro, for guaranteeing explant genetic diversity, over seven treatments. Treatment 5, included PLATIBACGEL as the main constituent, improved and sustained all in vitro seed germination, root penetration, and plant support. Likewise, repeated tomato micropropagation subcultures were successful. Results demonstrated applying PLATIBACGEL as a promising, reusable, cheap, and reliable alternative plant micropropagation media gelling agent. Wherefore, plant cellular developers and tissue-culturists can utilize bio-polymers like BC for better understanding plant cell response to different in vitro culturing conditions, with expected beneficial returns on gelling agents industry and markets as well. Graphical abstract

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Section: Mechanical Strengthsupporting

confidence: 88%

Developed applicability of a bacterial cellulose matrix as a gelling substitute for plant tissue culture media

et al. 2022

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“…Explant type, the constituents of media and genotype are important items affecting the success of in vitro plant regeneration. Several other agents in in vitro regeneration are sugar sources, gelling agent, and growth ingredients [ 180 , 181 , 182 ]. The process of in vitro plant regeneration begins with the formation of callus and then ends with the induction of shoot formation.…”

Section: Physiological Responses Of Plant Regenerationmentioning

confidence: 99%

Cellular, Molecular, and Physiological Aspects of In Vitro Plant Regeneration

Bidabadi

Jain

2020

Plants

128

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Plants generally have the highest regenerative ability because they show a high degree of developmental plasticity. Although the basic principles of plant regeneration date back many years, understanding the cellular, molecular, and physiological mechanisms based on these principles is currently in progress. In addition to the significant effects of some factors such as medium components, phytohormones, explant type, and light on the regeneration ability of an explant, recent reports evidence the involvement of molecular signals in organogenesis and embryogenesis responses to explant wounding, induced plant cell death, and phytohormones interaction. However, some cellular behaviors such as the occurrence of somaclonal variations and abnormalities during the in vitro plant regeneration process may be associated with adverse effects on the efficacy of plant regeneration. A review of past studies suggests that, in some cases, regeneration in plants involves the reprogramming of distinct somatic cells, while in others, it is induced by the activation of relatively undifferentiated cells in somatic tissues. However, this review covers the most important factors involved in the process of plant regeneration and discusses the mechanisms by which plants monitor this process.

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“…Dicamba produced the highest callus weight at the third week, which was earlier compared to 2,4-D and picloram. This is due to the already reported ability of dicamba to initiate the growth of callus even after two to three days of culturing on nutrient medium [ 49 ]. The use of dicamba as a single PGR in research on Centella asiatica was reported to be effective in promoting callus growth [ 9 ].…”

Section: Discussionmentioning

confidence: 99%

“…This plant grows in swampy areas of tropical and subtropical regions of the world [ 51 ]. According to Malik et al [ 49 ], the rate of callus growth induced by dicamba increased with the increase of its concentration. This is contrary to the present study since the higher concentrations of dicamba at 40 and 50 μM reduced the growth of P. bullata callus.…”

Section: Discussionmentioning

confidence: 99%

Induction, Multiplication, and Evaluation of Antioxidant Activity of Polyalthia bullata Callus, a Woody Medicinal Plant

Zaman

Azzeme

Ramle

et al. 2020

Plants

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Polyalthia bullata is an endangered medicinal plant species. Hence, establishment of P. bullata callus culture is hoped to assist in mass production of secondary metabolites. Leaf and midrib were explants for callus induction. Both of them were cultured on Murashige and Skoog (MS) and Woody Plant Medium (WPM) containing different types and concentrations of auxins (2,4-dichlorophenoxyacetic acid (2,4-D), α-naphthaleneacetic acid (NAA), picloram, and dicamba). The callus produced was further multiplied on MS and WPM supplemented with different concentrations of 2,4-D, NAA, picloram, dicamba, indole-3-acetic acid (IAA), and indole-3-butyric acid (IBA) media. The quantification of total phenolic content (TPC), total flavonoid content (TFC) and antioxidant capacity was further carried out on P. bullata callus, and the results were subjected to correlation analysis. Among the media, the WPM + 16.56 µM picloram (53.33 ± 22.06%) was the best for callus induction while MS + 30 µM dicamba was the best for callus multiplication. The TPC, TFC, and EC50 of DPPH scavenging activity were determined at 0.657 ± 0.07 mg GAE/g FW, 0.491 ± 0.03 mg QE/g, and 85.59 ± 6.09 µg/mL in P. bullata callus, respectively. The positive correlation between DPPH scavenging activity with TPC was determined at r = 0.869, and that of TFC was at r = 0.904. Hence, the P. bullata callus has an ability to accumulate antioxidants. It therefore can be a medium for secondary metabolites production.

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Evaluation of carbon sources, gelling agents, growth hormones and additives for efficient callus induction and plant regeneration in Indian wheat (Triticum aestivum L.) genotypes using mature embryos

Cited by 12 publications

References 38 publications

Developed applicability of a bacterial cellulose matrix as a gelling substitute for plant tissue culture media

Developed applicability of a bacterial cellulose matrix as a gelling substitute for plant tissue culture media

Cellular, Molecular, and Physiological Aspects of In Vitro Plant Regeneration

Induction, Multiplication, and Evaluation of Antioxidant Activity of Polyalthia bullata Callus, a Woody Medicinal Plant

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