Induction, Multiplication, and Evaluation of Antioxidant Activity of Polyalthia bullata Callus, a Woody Medicinal Plant

Abstract

Polyalthia bullata is an endangered medicinal plant species. Hence, establishment of P. bullata callus culture is hoped to assist in mass production of secondary metabolites. Leaf and midrib were explants for callus induction. Both of them were cultured on Murashige and Skoog (MS) and Woody Plant Medium (WPM) containing different types and concentrations of auxins (2,4-dichlorophenoxyacetic acid (2,4-D), α-naphthaleneacetic acid (NAA), picloram, and dicamba). The callus produced was further multiplied on MS and WPM supplemented with different concentrations of 2,4-D, NAA, picloram, dicamba, indole-3-acetic acid (IAA), and indole-3-butyric acid (IBA) media. The quantification of total phenolic content (TPC), total flavonoid content (TFC) and antioxidant capacity was further carried out on P. bullata callus, and the results were subjected to correlation analysis. Among the media, the WPM + 16.56 µM picloram (53.33 ± 22.06%) was the best for callus induction while MS + 30 µM dicamba was the best for callus multiplication. The TPC, TFC, and EC50 of DPPH scavenging activity were determined at 0.657 ± 0.07 mg GAE/g FW, 0.491 ± 0.03 mg QE/g, and 85.59 ± 6.09 µg/mL in P. bullata callus, respectively. The positive correlation between DPPH scavenging activity with TPC was determined at r = 0.869, and that of TFC was at r = 0.904. Hence, the P. bullata callus has an ability to accumulate antioxidants. It therefore can be a medium for secondary metabolites production.

Anticancer and antibacterial flavonoids from the callus of Ampelopsis grossedentata; a new weapon to mitigate the proliferation of cancer cells and bacteria

“…44 7 carbon resonance signals at δ C 110.56, 120.56, 140.38, 146.59 and 169.47 were in agreement with 3,4,5-trihydroxybenzoic acid. 22 These carbon resonance signals revealed that compound 1 contained two structural units dihydromyricetin and 3,4,5-trihydroxybenzoic acid. To assign the relative configurations of compound 1 , the ROESY correlations between H-1/H-2′, H-1/H-6′, H-2/H-2′ and H-2/H-6′ were done; it indicated that H-1, H-2 H-2′, and H-6′ were cofacial (Fig.…”

“…However, the callus culture could be a realistic source of bioactive secondary metabolites production with no time and space limitation and the technique has been widely used as an alternative for the production of plant secondary metabolites. 22 Therefore, the current research was designed to establish the in vitro callus cultures of this species as an alternative bioactivity-based reproducible approach for extraction and isolation of key bioactive compounds. Further, we assessed the cytotoxicity and antibacterial potential of the callus extract on A549, 4T1 and NCI-H1975 and against a panel of Gram positive bacteria and Gram negative bacterial pathogens, respectively.…”

Biotechnology and In Vitro Culture as an Alternative System for Secondary Metabolite Production

“…Many fields can use this approach, which allows the production of high-value bioactive compounds such as pharmaceuticals, food, and cosmetics [151]. The quantity and quality of the obtained metabolites can be greatly influenced by various parameters such as the nature of the elicitor, its concentration, and the exposure time, Table 4 [152][153][154][155][156][157][158].…”

Medicinal Plant Extracts

“…The study of medicinal and aromatic plants identified by the authors will be carried out according to a selection of the most used species, based on the country or area where the research is being conducted [ 4 ]. As such, in this special edition, the authors will be able to demonstrate their scientific contributions through the botanical description of medicinal plants—both traditionally and currently used species—as well as the chemical characterization of their respective phytocomplexes and their proven pharmacological action [ 5 , 6 ].…”