Evaluation of Biologically Active Compounds from Calendula officinalis Flowers using Spectrophotometry

Butnariu, Monica; Coradini, C. Z.

doi:10.1186/1752-153x-6-35

Cited by 101 publications

(86 citation statements)

References 33 publications

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“…1). The reason for colour change was the excitation of Surface Plasmon vibrations in metal nanoparticles [17,11]. Optical absorption spectroscopy has proved to be a very useful technique for the analysis of nanoparticles.…”

Section: Resultsmentioning

confidence: 99%

Biogenic Synthesis, Characterization of Silver Nanoparticles from Candelula Officinalis Extract and Evaluation of Their Antimicrobial Activity

Rashmi¹,

Grewal²

2015

Nano BioMed ENG

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Synthesis of silver nanoparticles from root extract of Candelula officinalis (marigold) as a biomaterial is reported in the present study. Plant root extract has water soluble organic materials which help in the reduction of silver ions and stabilization of AgNPs. Aqueous solution of silver nitrate was treated with root extract of C. officinalis for the formation of AgNPs. The UV-Visible spectroscopy indicated an absorption peak at 423 nm, which indicates synthesis of AgNPs. Further characterization by Dynamic Light Scattering (DLS) showed size distribution profile of synthesized silver nanoparticles (AgNPs). Transmission electron microscopy (TEM) demonstrated the presence of spherical AgNPs in range of 14-40 nm. The formed silver nanoparticles showed good antimicrobial activity against various microorganisms viz. Eshcherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, Candida albicans. AgNPs showed the maximum activity against Pseudomonas aeruginosa with a inhibition zone of 22 nm. Thus, the present study provides evidence of simple, rapid and environmental friendly route to synthesize AgNPs using aqueous root extract of Candelula officinalis.

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Section: Resultsmentioning

confidence: 99%

Biogenic Synthesis, Characterization of Silver Nanoparticles from Candelula Officinalis Extract and Evaluation of Their Antimicrobial Activity

Rashmi¹,

Grewal²

2015

Nano BioMed ENG

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“…These activities are related to the chemical composition of the plant, especially the presence of flavonoids and polyphenols (Butnariu & Coradini 2012;Borella et al 2010). According to the results obtained for the HECO, a potential antioxidant and anti-inflammatory effect is also exhibited.…”

Section: Discussionmentioning

confidence: 99%

“…Among its constituents, phenolic compounds (flavonoids and phenolic acids), along with saponins, carotenoids, and triterpenic alcohols are presented (Parente et al 2004;Butnariu & Coradini, 2012). The flavonoids include quercetin, rutin, narcissin, isorhamnetin and kaempferol (Fonseca et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Phytochemical evaluation and in vitro antioxidant and photo-protective capacity of Calendula officinalis L. leaves

Deuschle

Piana

et al. 2015

Rev. bras. plantas med.

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ABSTRACT:The plant Calendula officinalis L. is widely applied due to its medicinal properties, which are mainly dermatological and ornamental. The goal of this study is to assess the phytochemical components in a hydroethanolic extract (HECO) from the leaves of Calendula officinalis L. using UV-VIS spectrophotometry and thin layer chromatography (TLC), as well as to identify and quantify the components related to its antioxidant capacity employing high performance liquid chromatographic (HPLC). The antioxidant capacity evaluation was performed using the DPPH method for superoxide and hydroxyl radicals. The photo-protective capacity was evaluated by UVspectrophotometry in order to determine the in vitro Sun Protection Factor(SPF). The results show the plant's strong antioxidant activity (DPPH and hydroxyl methods), which we believe to be related to the presence of flavonoids (24.67 mg/g), polyphenols (33.90 mg/g), condensed tannins (27.30 mg/g), and the amount of rutin (37.25 mg/g), and quercetin (6.09 mg/g) found during the study. The HECO presented a good antioxidant capacity, most likely due to the polyphenols, flavonoids, and tannins in its contents. However, the obtained SPF of 1.89 ± 0.05 does not allow the plant to be classified as a stand-alone sunscreen, and more studies are needed in order to test its ability to enhance sunscreens in existing cosmetic formulations.Keywords: Calendula officinalis. Marigold. Antioxidant. Phenolic Compounds. Photo-protection. RESUMO:Avaliação fitoquímica e capacidade antioxidante e fotoprotetora in vitro das folhas de Calendula officinalis L. A Calendula officinalis L. é uma planta amplamente utilizada por suas propriedades medicinais, principalmente dermatológica e ornamental. O objetivo deste estudo é avaliar constituintes fitoquímicos do extrato hidroetanólico das folhas de Calêndula officinalis L. (HECO) por espectrofotometria UV-visível e cromatografia em camada delgada (CCD), bem como detectar e quantificar os componentes relacionados com a capacidade antioxidante por Cromatografia Líquida de Alta Eficiência (CLAE). Posteriormente, foi avaliada a capacidade antioxidante pelo método do DPPH, radicais superóxido e hidroxila. Além disso, a capacidade fotoprotetora foi avaliada através de espectrofotometria UV para determinação do Fator de Proteção Solar in vitro (FPS). Os resultados evidenciaram que a planta apresentou uma excelente atividade antioxidante para o método do DPPH e do radical hidroxila, o que pode estar relacionada com a presença de flavonoides (24,67 mg/g), polifenóis (33,90 mg/g) e taninos condensados (27,30 mg/g) e da quantidade de rutina (37,25 mg/g) e quercetina (6,09 mg/g) encontrados neste estudo. O HECO apresentou uma notável capacidade antioxidante, provavelmente devido à presença de polifenóis, flavonoides e taninos. O valor do FPS encontrado (1,89 ± 0,05), não classifica a planta como um protetor solar isolado. No entanto, mais estudos são necessários para testar a capacidade de potencializar filtros solares em formulações cosméticas.

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“…Dobijeni rezultati su u skladu sa prethodnim ispitivanjima, gde su utvrđeni heterozidi izoramnetina i kvercetina: rutin, izoramnetin-3-O-rutinozid, 3-O-glukozid izoramnetina, izokvercitrin, kao i kafena, hlorogenska i galna kiselina (7,19).…”

Section: Analiza Flavonoida Pomoću Hplc Metodeunclassified

Investigation of quality and antimicrobal activity of cultivated marigold flowers Calendula officinalis L.

Gurzulov¹,

Marčetić²,

Božić³

2016

Medicinski podmladak

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Evaluation of Biologically Active Compounds from Calendula officinalis Flowers using Spectrophotometry

Cited by 101 publications

References 33 publications

Biogenic Synthesis, Characterization of Silver Nanoparticles from Candelula Officinalis Extract and Evaluation of Their Antimicrobial Activity

Biogenic Synthesis, Characterization of Silver Nanoparticles from Candelula Officinalis Extract and Evaluation of Their Antimicrobial Activity

Phytochemical evaluation and in vitro antioxidant and photo-protective capacity of Calendula officinalis L. leaves

Investigation of quality and antimicrobal activity of cultivated marigold flowers Calendula officinalis L.

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