Investigation of quality and antimicrobal activity of cultivated marigold flowers Calendula officinalis L.

Gurzulov, Katarina; Marčetić, Mirjana; Božić, Dragana

doi:10.5937/medpodm1602073g

Cited by 2 publications

(3 citation statements)

References 8 publications

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“…The TFC depends on the extraction techniques, solvents, and used procedures for its determination. The extract of pot marigold prepared by maceration using 70% (v/v) ethanol has the TFC of 1.45% [1]. Jurca et al [12] defined the TFC as milligram quercetin equivalent per 100 g of the dry weight (mg QE 100 g -1 d.w.).…”

Section: (1) (2019) 10-18mentioning

confidence: 99%

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The optimization of ultrasound-assisted extraction of total flavonoids from pot marigold (Calendulae officinalis L.) flowers

Zerajić¹,

Savić-Gajić²,

Savić³

et al. 2019

Adv Techol

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Pot marigold (Calendula officinalis L.) contains various bioactive compounds such as flavonoids, tannins, saponosides, carotenoids, triterpene alcohols, polysaccharides, amino acids, and essential oil. The extract of pot marigold flower has a benefit to the skin due to the presence of flavonoids that possess the anti-inflammatory, astringent, antifungal, and antiseptic effects. The aim of this study was to apply the ultrasound-assisted extraction as an advanced extraction technique and to investigate the impact of extraction time (5-35 min), ethanol concentration (20-80%, v/v), and extraction temperature (30-70 °C) on the total flavonoid content of pot marigold flower at the liquid-to-solid ratio of 20 cm 3 g -1 . The total flavonoids content of the extracts was determined spectrophotometrically with aluminium (III) chloride. The modeling of ultrasound-assisted extraction was carried out using a Box-Behnken design. Thus found optimal extraction conditions were the extraction time of 29 min, 39.6% (v/v) ethanol, and extraction temperature of 64.2 °C. The experimental value (220.2 mg 100 g -1 d.w.) of the total flavonoid content under optimal conditions was in a good agreement with the predicted value (221.5 mg 100 g -1 d.w.). According to the results of statistical analysis, the proposed second-order polynomial equation can be used to describe the extraction of flavonoids and to predict the total flavonoid content. The extraction procedure can be accepted from the point of the pharmaceutical application due to the use of ethanol as a representative of green solvents. The extraction time was shorter compared with other conventional extraction techniques.

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Section: (1) (2019) 10-18mentioning

confidence: 99%

“…Pot marigold (Calendula officinalis L.) originates from the Mediterranean [1], and grows in the gardens and parks of Central and Southern Europe, Western Asia and USA [2]. It reaches a height between 30 and 60 cm [2,3].…”

Section: Introductionmentioning

confidence: 99%

The optimization of ultrasound-assisted extraction of total flavonoids from pot marigold (Calendulae officinalis L.) flowers

Zerajić¹,

Savić-Gajić²,

Savić³

et al. 2019

Adv Techol

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show abstract

“…It is used for the treatment of dry and damaged skin, although it can be used for all skin types. Today, the traditional ointments with ethanolic extract of dried pot marigold flower are still used [10]. The intensive studies have focused on the development of new carriers for the topical application of pot marigold flower extract [11].…”

Section: Introductionmentioning

confidence: 99%

Physical, chemical, and antioxidant stability studies of a topical formulation containing pot marigold (Calendula officinalis L.) flowers extract

Savić¹,

Savić-Gajić²

2021

Adv Techol

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Pot marigold flower extract (Calendula officinalis L.) has pharmacological properties due to the presence of various bioactive compounds. It is known that the extract has antioxidant, anti-inflammatory, antitumor, antibacterial, antifungal, antiviral, antimutagenic, antidermatitis properties, etc. The aim of this study was to improve the quality of the selected topical formulation by adding the ethanolic extract of pot marigold flower, as well as to monitor its stability. The topical formulation was water-in-oil emulsion prepared using the hot/hot emulsification process with an oil phase consisting of Vaseline, lanolin, and almond oil. The extract, prepared by ultrasound-assisted extraction, had an antioxidants content of 3.512 g gallic acid equivalent per 100 g-1 of dry weight and the half-maximal inhibitory concentration of 0.14 mg mL-1 determined by the DPPH assay. Chemical stability studies have shown that daylight has no significant effect on the stability of antioxidants in the extract, while an increase in temperature leads to their degradation. The shelf-life of the extract is about 8 months at 4 °C and 3 months at 22 °C (room temperature). The prepared uncategorized topical formulations containing 1% and 2% (w/w) pot marigold extract were stable at different temperatures during the storage. The uncategorized formulations showed antioxidant activity, but the activity of the extract in the formulations decreased with increasing storage temperature. Pot marigold flower extract and the developed uncategorized formulations showed an inhibitory effect on Gram-positive (Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli, Proteus mirabilis, Klebsiella pneumoniae), as well as on Candida albicans. The uncategorized formulations with this activity can be used in the treatment of skin infection.

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Investigation of quality and antimicrobal activity of cultivated marigold flowers Calendula officinalis L.

Cited by 2 publications

References 8 publications

The optimization of ultrasound-assisted extraction of total flavonoids from pot marigold (Calendulae officinalis L.) flowers

The optimization of ultrasound-assisted extraction of total flavonoids from pot marigold (Calendulae officinalis L.) flowers

Physical, chemical, and antioxidant stability studies of a topical formulation containing pot marigold (Calendula officinalis L.) flowers extract

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