Evaluation of basal media for micropropagation of four highbush blueberry cultivars

Tetsumura, Takuya; Matsumoto, Yasuyo; Sato, Makiko; Honsho, Chitose; Yamashita, Kensuke; Komatsu, Hirokazu; Sugimoto, Yasuhiro; Kunitake, Hisato

doi:10.1016/j.scienta.2008.06.028

Cited by 61 publications

(51 citation statements)

References 12 publications

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“…When the percentages of shoot formation between V. arctostaphylos and V. myrtillus were compared, no dramatic difference was observed with the exception that the shoots of the former were significantly longer (Cüce et al, 2013) than the latter (presented here). Although zeatin was also found to be effective for shoot proliferation of lowbush blueberry (Kaldmäe et al, 2006), lingonberry (Debnath and McRae, 2001b), and highbush blueberry (Chandler and Draper, 1986;Eccher and Noe, 1989;Tetsumura et al, 2008), 2iP supplemented with IBA significantly enhanced axillary shoot elongation in highbush blueberry (Litwińczuk and Wadas, 2008). In agreement with this, the highest shoot elongation of the present study (13.01 mm) was observed when WPM plus 1.0/0.1 mg L -1 zeatin/IBA combination was employed.…”

Section: Discussionmentioning

confidence: 99%

Micropropagation of Vaccinium myrtillus L. (Bilberry) naturally growingin the Turkish flora

Cüce¹,

Sökmen²

2015

Turk J Biol

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This study was designed to micropropagate Vaccinium myrtillus L., a naturally growing bilberry, in the Turkish flora. In order to determine the most effective basal medium, lateral buds were initially selected as explants, which were then individually cultured in Murashige and Skoog medium, Anderson's rhododendron medium, and McCown's woody plant medium (WPM) supplemented with 1.0 mg L -1 zeatin in combination with either 0.1 mg L -1 indole-3-butyric acid (IBA) or 0.1 mg L -1 α-naphthalene acetic acid. WPM, supplemented with zeatin and IBA, was found to be the most effective basal medium tested. As shoot regeneration ability highly depends on the medium and cytokinin concentration, WPM basal media containing various concentrations (0.5, 1.0, and 2.0 mg L -1 ) of 3 different cytokinins, namely zeatin, thidiazuron, and N 6 -[2-isopentenyl] adenine, were employed together with IBA (0.1 mg L -1 ) and were compared with basal media containing none of the growth regulators. In terms of shoot multiplication, 2.0 mg L -1 zeatin was found to be superior to the other tested growth regulators when combined with 0.1 mg L -1 IBA. WPM was also used as a basal medium for rooting and was supplemented with different concentrations of IBA (0.25-2.0 mg L -1 ) with or without activated charcoal (AC). WPM supplemented with 0.5/1.0 mg L -1 IBA/AC was the best culture medium for rooting with 60% root formation.

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Section: Discussionmentioning

confidence: 99%

Micropropagation of Vaccinium myrtillus L. (Bilberry) naturally growingin the Turkish flora

Cüce¹,

Sökmen²

2015

Turk J Biol

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“…However, visual inspections showed that the release of phenolics was enhanced when explants were cultured in MS, which is possibly due to the higher concentration of salt in this medium versus the WPM culture medium. Tetsumura et al (2008), Mereti et al (2002) and Andreu and Marin (2005) found that the efficiency of the morphogenic response of plants in different culture media was dependent on the genotype of the species used, the concentration of salts present in the culture medium, and the species itself.…”

Section: Evaluation Of the Culture Media And The Addition Of Growth Rmentioning

confidence: 99%

In vitro culture of Luma chequen from vegetative buds

et al. 2013

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“…Este medio se formuló emulando la composición de macronutrientes de WPM [14], en tanto que se mantuvo sin cambios las concentraciones de micronutrientes. El pH del medio fue 5.2 y la concentración de sacarosa de 20 g/l [15,16,17].…”

Section: Germinación In Vitro De Semillas De Mortiñounclassified

“…Tetsumura et al [16] también observaron un porcentaje de contaminación de hasta 40 %, utilizando explantes de plantas de blueberry mantenidas en un espacio abierto. Los explantes de mortiño se obtuvieron de plantas silvestres que estaban creciendo en su hábitat natural, lo cual podría explicar el alto porcentaje de contaminación que se observó.…”

Section: Introducción In Vitro Y Brotación De Yemas Axilares De Mortiñounclassified

Cultivo in vitro del mortiño (<em>Vaccinium floribundum</em> Kunth)

Torres

Trujillo

Arahana

2010

Av. Cienc. Ing. (Quito)

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Due to the economic and agronomic potencial that mortiño (Vaccinium floribundum Kunth) has in Ecuador, the objective of this research was to establish in vitro propagation methods for this species by seed germination and by shoot growth from axillary buds. Modified Woody Plant Medium [1] (mWPM) was used, in which up to 60.7 % of seeds germinated. A strong cytokine, trans-zeatin riboside, TZR (1 mg/l), combined with α-napthaleneacetic acid, NAA (0.05 mg/l), was used to subculture the in vitro germinated plantlets. The use of basal medium without hormones or with 2iP, 6-(gamma,gamma-dimethylalylamino) purine, allowed elongation and rooting of plantletss. The acclimatization of mortiño plants has not yet been standardized, and further research is needed to find the conditions that are suitable for this process. To establish in vitro cultures by axillary buds, apical segments of the stem were disinfected and introduced in medium mWPM containing TZR, (7 mg/l) combined with NAA (0.1 mg/l). Growing buds were transferred to media with 2iP (3 or 5 mg/l) for propagation and elongation. This investigation is a preliminary study for the in vitro culture of mortiño, setting the basis for future research with this species.Keywords. trans-zeatin riboside (TZR), 6-(gamma,gamma-dimethylalylamino) purine (2iP), Vaccinium floribundum Kunth, in vitro germination, axillary buds. ResumenDebido al potencial agrícola y económico que tiene el mortiño (Vaccinium floribundum Kunth) para el Ecuador, esta investigación tuvo como objetivo establecer métodos de propagación in vitro de esta especie como germinación de semillas y brotación de yemas axilares. Se utilizó un medio basal sin hormonas, Woody Plant Medium [1] modificado (mWPM), en el que las semillas germinaron con hasta un 60.7 % de eficiencia. Para el subcultivo de plántulas germinadas in vitro , se usó una citoquinina fuerte, la trans zeatina ribóside, TZR (1 mg/l) en combinación con ácido α-naftalenacético, NAA (0.05 mg/l). El medio basal sin hormonas o con 2iP, 6-(gamma,gamma-dimetilalilamino) purina, permitió la elongación de las plántulas germinadas in vitro y su enraizamiento. La aclimatación de las plantas de mortiño cultivadas in vitro aún no está estandarizada y se requiere mayor investigación para encontrar los factores necesarios para completar satisfactoriamente este proceso. Para la introducción in vitro de yemas axilares se desinfectaron los extremos apicales de tallos de mortiño, y se los introdujo en medio mWPM suplementado con TZR (7 mg/l) en combinación con NAA, (0.1 mg/l). Las yemas brotadas se subcultivaron en medios que contenían 2iP (3 ó 5 mg/l) para su propagación y elongación. Esta investigación representa un estudio piloto para el cultivo in vitro del mortiño, sentando las bases para futuras investigaciones con esta especie.Palabras Clave. trans zeatina ribóside (TZR), 6-(gamma,gamma-dimetilalilamino) purina (2iP) , Vaccinium floribundum Kunth, germinación in vitro, brotación de yemas axilares. IntroducciónEl mortiño ( Vaccinium floribundum Kunth) e...

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Evaluation of basal media for micropropagation of four highbush blueberry cultivars

Cited by 61 publications

References 12 publications

Micropropagation of Vaccinium myrtillus L. (Bilberry) naturally growingin the Turkish flora

Micropropagation of Vaccinium myrtillus L. (Bilberry) naturally growingin the Turkish flora

In vitro culture of Luma chequen from vegetative buds

Cultivo in vitro del mortiño (<em>Vaccinium floribundum</em> Kunth)

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