Evaluation of autotrophic and mixotrophic regimen Chlorella pyrenoidosa cells in various wastes water for its biochemical composition and biomass production

Abstract

The present study investigates the possibility of integrating an existing industrial large scale biomass production with the treatment of waste water in which a mixture of organic and inorganic rich pollutants was used as a medium. This study suggests that the replacement of a defined medium with a complete mixotrophic medium gives a significant statistical difference in terms of growth parameters i.e. biomass production and specific growth rate.The green microalga C. pyrenoidosa was cultivated under different mixotrophic conditions for evaluation of biomass production. Inorganic defined fog's medium supplemented, with raw dairy wastewater led to 1.37g/L biomass production in comparison to 1.2g/L obtained with pure glucose revealing 14.16% increase. The study also involves the supplementation of raw dairy wastewater as an organic carbon source in an inorganic medium comprising municipal treated water and reverse osmosis (RO) treated wastewater and attained 2.4g/L and 1.6g/L of biomass respectively, as compared to 0.3g/L and 0.16g/L obtained in the wastewaters alone revealing 700% and 900% increase respectively. Mixotrophic regimen cells as analyzed by a 2D Fourier transform infrared (FTIR) spectroscopy for its biochemical content revealed that fog's blended raw dairy waste (RDW) regimen cells had maximum Carbohydrate/Amide ratio. The study suggests that the mixotrophic regimen C. pyrenoidosa cells can show appropriate growth in a mixture of waste waters and the same comes out to be a cost effective and feasible alternative commercial medium for biomass production without requiring any expensive organic carbon sources in the culture medium.

Investigating the Physiological Responses of Three Endaphic Strains of Cyanobacteria to Crude Oil Concentrations in Limited Salinity and Irradiation Conditions

“…On the other hand, the dry weight contents at different concentrations of crude oil were classified into three categories, which showed a significant difference, so that the control concentration had the lowest value and concentrations of 1-5% crude oil shows the highest amount (Table 4). Dhull et al (2014) found that by increasing the concentration of carbon in the medium, the biomass production rate increases in some microalgae species, which is consistent with the results of this study.…”

Glucose addition‐induced changes in the growth and chemical compositions of a freshwater microalga Chlorella kessleri

“…In the mixotrophic cultures, the content of protein in C. kessleri decreased from 54.3% to 34.9%, whereas that of carbohydrate increased from 12.6% to 28.2% with the increase of glucose concentrations from 2 to 15 g L −1 . In agreement with the present study, the same changes of protein and carbohydrate contents in C. pyrenoidosa were observed by Dhull et al ., who found that the contents of protein and carbohydrate were 65.0%, 20.3% and 39.3%, 54.0%, when C. pyrenoidosa grew in the photoautotrophic and mixotrophic cultures, respectively. Moreover, the content of lipids in C. kessleri increased firstly from 25.0% to 32.7% with increasing glucose concentrations from 2 to 6 g L −1 , and then maintained at c .32.0% when the glucose concentrations were > 6 g L −1 .…”

Cultivation of the Microalgae Chlorella Pyrenoidosa Using the Processes of Biotechnology

“…The cellular concentration for C. pyrenoidosa was determined by withdrawing 5 ml of microalgae suspension throughout the experiment (8 days) via the test tube. In this way, optical density measurements were carried out using a FEMTO 700 PLUS spectrophotometer, whose reading wavelength was 686nm, and the following equation was used Growth rate (wet biomass) = (observed optical density / 0.0563) (R 2 = 0.9989) (DHULL et al, 2014).…”