A Deus e às leis da natureza_física (3ª Lei de Newton, principalmente). Às pessoas verdadeiras que encontrei ao longo da Vida, e quem torce por mim de verdade. Dedico essa pesquisa a todas as pessoas que me incentivam e motivam em todos os dias de minha Vida. v Agradecimentos Ao CNPQ, pela bolsa de doutorado concedida, pois sem esta seria impossível realizar este trabalho. Aos meus pais, Shyrlei e Helson, por terem me proporcionado a Vida e me fazerem enxergar que todos nós possuímos defeitos e qualidades. Por me fazerem acreditar em mim, em meus potenciais, e em Deus, que nos guia, abençoa e orienta. Ao meu irmão Fábio, mestre em Metrologia na área de Eng a Elétrica (UFSC-SC), pelo incentivo aos estudos. À minha irmã Fiorella, mestra em Letras (USP-SP), docente na área de Língua Portuguesa e Alemã, exemplo de dedicação e horas de estudo. À minha única avó viva, Olívia, linda, feliz, bondosa, que me proporcionou muitas orientações, ensinamentos e muitos conselhos de Vida. E também, por me fazer acreditar que devemos orar, agradecer e pedir a Deus para nos ajudar a seguir nossa jornada diária. In memorium: Américo, Aristides, Cristina e Hercílio. Enfim, a todos os meus ancestrais. Aos meus padrinhos, Paulo e Sara, os quais me batizaram quando pequenina, e a seus filhos Paulo e Pedro, pela existência em minha Vida e serem exemplos de caráter, disciplina e amor a seguir.

The results obtained here, show that the use of Chlorella pyrenoidosa microalgae and biotechnology, using the discontinuous process, presented satisfactory results. With this, the study of the microalga Chlorella sp. has proved to be important because it has a wealth of proteins, carbohydrates, amino acids, fatty acids, carotenoids, vitamins and minerals in its constitution, which may represent commercial importance. This research revealed the best results for obtaining a lipoprotein-rich biomass, taking into account three different culture media, calculations of cell concentration, cell productivity, to the content (%) of protein, lipid, carbohydrate and ash present in the microalgal biomass.

The use of naturally occurring nanobiotechnological inputs is essential for human health and the environment. This research is of fundamental importance, given consumers' current awareness of using more natural, sustainable and healthy pharmaceutical ingredients. Thus, there has been a growing demand for more biocompatible pharmaceutical formulations with the body, which should have on their labels no aggression to nature (environment) and no use of animal testing (USP-2018). Thus, consumers want to direct the benefits that come from nature, among other means of knowledge, to their families. They are seeking to associate the cost of the finished cosmetic product with the benefit it brings to the health of the human body. In this research the continuation of studies of scientists Araújo, Giudici and Sousa, in 2019 a, b and c, which used this patented nanobiotechnological input (USP-2018), was used in the natural formulation of liquid soap.

Through the cultivation of the microalgae Chlorella pyrenoidosa was used the discontinuous biotechnological process, which was obtained a microalgal biomass (dry). This was directed to the supercritical processing apparatus, in which after use it was obtained an extract.  This was analyzed to verify the types (varieties) of fatty acids in a gas chromatograph and also, it was coupled to mass spectrometry. It was noted that to perform the analysis in these two devices there was a complementarity between the results of types of fatty acids.

The use of naturally occurring nanobiotechnological inputs is essential for human health and the environment. This research is of fundamental importance, given consumers' current awareness of using more natural, sustainable and healthy pharmaceutical ingredients. Thus, there has been a growing demand for more biocompatible pharmaceutical formulations with the body, which should have on their labels no aggression to nature (environment) and no use of animal testing (Araújo & Giudici, 2018). Thus, consumers want to direct the benefits that come from nature, among other means of knowledge, to their families. They are seeking to associate the cost of the finished cosmeticproduct with the benefit it brings to the health of the human body. In this research the continuation of studies of the scientist Araújo, which used the patented nanobiotechnological input (Araújo & Giudici, 2018), was used in the natural formulation (Algal nanobiotechnological refreshing spray for the face).