Evaluation of Assays for Measurement of Serum (Anti)oxidants in Hemodialysis Patients

Ruskovska, Tatjana; Jansen, Eugène; Antarorov, Risto

doi:10.1155/2014/843157

Cited by 16 publications

(11 citation statements)

References 36 publications

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“…Although ESRD patients who are dependent on chronic haemodialysis treatment are widely recognised as having an oxidative stress related condition, the lack of significant difference in overall protein oxidation results are consistent with others [39]. The decrease in total protein oxidation after vitamin C intervention is again in accordance with our previous study; combined vitamin C / vitamin E antioxidant supplementation resulted in significant decrease of the plasma hydroperoxides of nearly 70% in chronically haemodialysed patients [40].…”

Section: Discussionsupporting

confidence: 90%

Ankyrin is the major oxidised protein in erythrocyte membranes from end-stage renal disease patients on chronic haemodialysis and oxidation is decreased by dialysis and vitamin C supplementation

Ruskovska

Bennett

Brown

et al. 2014

Free Radical Research

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Chronically haemodialysed end-stage renal disease patients are at high risk of morbidity arising from complications of dialysis, the underlying pathology that has led to renal disease and the complex pathology of chronic kidney disease. Anaemia is commonplace and its origins are multifactorial, involving reduced renal erythropoietin production, accumulation of uremic toxins and an increase in erythrocyte fragility.Oxidative damage is a common risk factor in renal disease and its co-morbidities, and is known to cause erythrocyte fragility. Therefore we have investigated the hypothesis that specific erythrocyte membrane proteins are more oxidised in endstage renal disease patients and that vitamin C supplementation can ameliorate membrane protein oxidation.Eleven patients and fifteen control subjects were recruited to the study. Patients were supplemented with 2 x 500mg vitamin C per day for four weeks. Erythrocyte membrane proteins were prepared pre-and post-vitamin C supplementation for determination of protein oxidation. Total protein carbonyls were reduced by vitamin C supplementation but not by dialysis when investigated by enzyme linked immunosorbent assay. Using a western blot to detect oxidised proteins, one protein band, later identified as containing ankyrin, was found to be oxidised in patients but not controls and was reduced significantly by 60% in all patients after dialysis and by 20% after vitamin C treatment pre-dialysis. Ankyrin oxidation analysis may be useful in a stratified medicines approach as a possible marker to identify requirements for intervention in dialysis patients.

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Section: Discussionsupporting

confidence: 90%

Ankyrin is the major oxidised protein in erythrocyte membranes from end-stage renal disease patients on chronic haemodialysis and oxidation is decreased by dialysis and vitamin C supplementation

Ruskovska

Bennett

Brown

et al. 2014

Free Radical Research

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“…In a study of biomarkers to assess OS in hemodialysis patients in comparison to healthy people [22], the authors concluded that the variability of results obtained using different assays “should be taken into account when interpreting data from clinical studies of OS”, in agreement with the hypothesis of different types of OS [8]. This is “especially (true) in complex pathologies” [22], which can be attributed to the association of different types of OS to different pathologies. In another study, the same authors showed that the inter-assay correlations between different antioxidant assays were difficult to interpret, since some assays showed nice correlation while others showed no correlation [23].…”

Section: Discussionmentioning

confidence: 99%

Gender- and age-dependencies of oxidative stress, as detected based on the steady state concentrations of different biomarkers in the MARK-AGE study

et al. 2019

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Recently, Weber et al. published a thorough investigation of the age-dependency of oxidative stress (OS) determined by the steady state concentrations of different compounds - oxidation products and antioxidants - that are in common use as biomarkers of OS in 2207 healthy individuals of the cross-sectional MARK-AGE Project. The correlations among biomarkers were significant but weak. These findings may indicate different manifestations of OS and must further be evaluated. Here, we report a refined analysis of OS based on the above-mentioned original data. We show that malondialdehyde (MDA) appears to be sensitive to both gender and age. It is significantly lower and shows a greater age-dependence in women than in men. The age-dependency of MDA in women arises in a stepwise fashion. The age-dependent slope of the steady state concentration is maximal at the age between 50 and 55 years, indicating that it may be attributed to the change of metabolism in the post-menopause. Interestingly, total glutathione (GSH) decreased with age simultaneously with the increase in MDA. Different biomarkers yield different gender- and age-dependencies. Unlike the concentration of MDA, the concentrations of the other two oxidation products, i.e. protein carbonyls and 3-nitrotyrosine were similar in men and women and appeared to be independent of age in the healthy study population. The analyzed antioxidants exhibited different gender- and age-dependencies. In conclusion, it appears that all the biomarkers assessed here reflect different types of OS and that MDA and GSH reflect the same type of OS.

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“…There are different studies which demonstrated the total serum antioxidant levels in chronic hemodialyzed patients compared to the healthy group [ 30 ]. Furthermore, some studies have found higher total serum antioxidant levels at predialysis [ 31 , 32 ], and some have found a significant decrease in antioxidants after dialysis [ 32 ]. Different results might be caused by different tests used for measurement of antioxidants and the results might be affected by many patient-related factors [ 7 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of Hemodialysis on Thiol-Disulphide Homeostasis in Critically Ill Pediatric Patients with Acute Kidney Injury

Ayar

Şahin

Yazıcı

et al. 2018

BioMed Research International

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Aim To evaluate thiol/disulphide homeostasis as a new indicator of oxidative stress in AKI patients and to determine the effect of HD on antioxidant balance and oxidative stress through plasma thiols. Methods This study was performed in patients aged between 12 months and 18 years prospectively who underwent hemodialysis due to AKI and were followed up for a year in a 22-bed tertiary pediatric intensive care unit. 20 patients and 39 controls were included. Results No difference was present between the groups in terms of age and gender. Median values of plasma native thiol, total thiol, and percent thiol were significantly lower in AKI group both before and after dialysis when compared to control group. The median dynamic disulphide values were significantly lower in the AKI group of predialysis compared to the controls. When pre- and postdialysis values were compared, disulphide values were statistically higher after dialysis. When pre- and postdialysis native thiol, dynamic disulphide, total thiol, and percent thiol median values were compared, postdialysis values were significantly higher than the predialysis values. There was a positive correlation between albumin, total thiol, and native thiol values before dialysis in the patient group. Conclusion AKI patients have low levels of thiol species showing the presence of oxidative stress and hemodialysis has a positive effect on thiol/disulphide balance. This new method may be an inexpensive and simple tool suitable for clinical studies and can be used in routine screening as a useful indicator to show oxidative stress.

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Evaluation of Assays for Measurement of Serum (Anti)oxidants in Hemodialysis Patients

Cited by 16 publications

References 36 publications

Ankyrin is the major oxidised protein in erythrocyte membranes from end-stage renal disease patients on chronic haemodialysis and oxidation is decreased by dialysis and vitamin C supplementation

Ankyrin is the major oxidised protein in erythrocyte membranes from end-stage renal disease patients on chronic haemodialysis and oxidation is decreased by dialysis and vitamin C supplementation

Gender- and age-dependencies of oxidative stress, as detected based on the steady state concentrations of different biomarkers in the MARK-AGE study

Effects of Hemodialysis on Thiol-Disulphide Homeostasis in Critically Ill Pediatric Patients with Acute Kidney Injury

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