Ankyrin is the major oxidised protein in erythrocyte membranes from end-stage renal disease patients on chronic haemodialysis and oxidation is decreased by dialysis and vitamin C supplementation

Abstract

Chronically haemodialysed end-stage renal disease patients are at high risk of morbidity arising from complications of dialysis, the underlying pathology that has led to renal disease and the complex pathology of chronic kidney disease. Anaemia is commonplace and its origins are multifactorial, involving reduced renal erythropoietin production, accumulation of uremic toxins and an increase in erythrocyte fragility.Oxidative damage is a common risk factor in renal disease and its co-morbidities, and is known to cause erythrocyte fragility. Therefore we have investigated the hypothesis that specific erythrocyte membrane proteins are more oxidised in endstage renal disease patients and that vitamin C supplementation can ameliorate membrane protein oxidation.Eleven patients and fifteen control subjects were recruited to the study. Patients were supplemented with 2 x 500mg vitamin C per day for four weeks. Erythrocyte membrane proteins were prepared pre-and post-vitamin C supplementation for determination of protein oxidation. Total protein carbonyls were reduced by vitamin C supplementation but not by dialysis when investigated by enzyme linked immunosorbent assay. Using a western blot to detect oxidised proteins, one protein band, later identified as containing ankyrin, was found to be oxidised in patients but not controls and was reduced significantly by 60% in all patients after dialysis and by 20% after vitamin C treatment pre-dialysis. Ankyrin oxidation analysis may be useful in a stratified medicines approach as a possible marker to identify requirements for intervention in dialysis patients.

Red blood cell abnormalities and the pathogenesis of anemia in end‐stage renal disease

“…peroxiredoxin‐2), protein aggregation/complexation (e.g. spectrin–Hb complex , fragmentation, oxidation /carbonylation (e.g. ankyrin ) and activation (e.g.…”

“…spectrin–Hb complex , fragmentation, oxidation /carbonylation (e.g. ankyrin ) and activation (e.g. of calpain following increase in cytosolic calcium) (Table ).…”

The Effect of Erythropoietin on Total Protein Levels during Ischemia Reperfusion Injury in Rats

“…Yang et al [4] found limited evidence to suggest that nandrolone decanoate can increase plasma TP (MD 0.40 g/L, 95% CI 0.13 to 0.67), levels compared with Epo. Ruskovska et al [5] found TP carbonyls reduced by vitamin C supplementation (2 × 500 mg vitamin C per day for 4 weeks) either under or not Epo dialysis in end-stage renal disease patients than control ones. The present authors found the Ischemia for 45 min followed by reperfusion for 60 min (group A); 2) Ischemia for 45 min followed by reperfusion for 120 min (group B); 3) Ischemia for 45 min followed by immediate Epo intravenous (IV) administration and reperfusion for 60 min (group C); 4) ischemia for 45 min followed by immediate Epo IV administration and reperfusion for 120 min (group D).…”

Vitamins E and C exert protective roles in hydrogen peroxide-induced DNA damage in human peripheral blood mononuclear cells

“…For example, ROS are involved in cell signaling, cell division, and protection against infections and carcinogenesis (MITTAL et al, 2014;SIES, 2017;PATTERSON et al, 2019). However, high levels of reactive species leading to oxidative distress are associated with lipid peroxidation, protein oxidation and DNA damage (RUSKOVSKA and BERNLOHR, 2013;NIKITAKI et al, 2015;RUSKOVSKA et al, 2015). On the other side, cellular antioxidant defense comprises enzymes that include superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, thioredoxins and others, and non-enzymatic antioxidants such as glutathione, carotenoids and the antioxidant vitaminsvitamin E and C (TRABER and STEVENS, 2011).…”