Effects of Hemodialysis on Thiol-Disulphide Homeostasis in Critically Ill Pediatric Patients with Acute Kidney Injury

Ayar, Ganime; Şahin, Şanlıay; Yazıcı, Mutlu Uysal; Neşeli̇oğlu, Salim; Erel, Özcan; Bayrakçı, Umut Selda

doi:10.1155/2018/1898671

Cited by 12 publications

(11 citation statements)

References 34 publications

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“…Previous research demonstrated that patients with hospital-acquired AKI had lower levels of unadjusted plasma-free thiols as compared to critically ill patients without AKI and healthy subjects [ 30 ]. In another study, pediatric patients with AKI had lower levels of unadjusted plasma-free thiols as compared to healthy controls [ 31 ]. Both the unadjusted thiol levels correlated with the albumin-adjusted thiol levels in our study; in contrast, we used adjusted thiol levels to correct for the albumin levels.…”

Section: Discussionmentioning

confidence: 99%

“…To date, to the best of our knowledge, no other study unravelled the association between (albumin-adjusted) plasma-free thiols and AKI in patients in the ICU with and without sepsis. Previous studies demonstrated lowered plasma-free thiol levels in children with AKI [ 31 ] or adult patients with AKI [ 30 ], although not among adult patients in the ICU. Moreover, as thiols are influenced by serum albumin levels, which might be affected by critical illness or sepsis, we corrected the thiol levels for albumin, in contrast to previous studies.…”

Section: Discussionmentioning

confidence: 99%

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Acute Kidney Injury is Associated with Lowered Plasma-Free Thiol Levels

et al. 2020

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Acute kidney injury (AKI) is associated with the abrupt loss of kidney function. Oxidative stress plays an important role in the pathophysiology of AKI. Free thiols (R-SH) are crucial components of the extracellular antioxidant machinery and reliably reflect systemic oxidative stress. Lower levels of thiols represent higher levels of oxidative stress. In this preliminary study, we hypothesized that plasma-free thiols are associated with AKI upon admission to the intensive care unit (ICU). In this study, 301 critically ill patients were included. Plasma samples were taken upon admission, and albumin-adjusted plasma-free thiols were determined. Albumin-adjusted plasma-free thiols were lower in patients with AKI (n = 43, median (interquartile range) 7.28 µmol/g (3.52, 8.95)) compared to patients without AKI (8.50 μmol/g (5.82, 11.28); p < 0.05) upon admission to the ICU. Higher age (B = −0.72), higher levels of neutrophil gelatinase-associated lipocalin (B = −0.002), creatinine (B = −0.01) and lower serum albumin (B = 0.47) were associated with lower free thiol levels. Further, albumin-adjusted free thiol levels were significantly reduced in patients with sepsis (8.30 (5.52–10.64) µmol/g) compared to patients without sepsis (6.95 (3.72–8.92) µmol/g; p < 0.05). Together, albumin-adjusted plasma-free thiols were significantly reduced in patients with AKI and patients with sepsis compared with patients without AKI and sepsis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Acute Kidney Injury is Associated with Lowered Plasma-Free Thiol Levels

et al. 2020

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“…Although it has been shown that thiols play a role in the pathogenesis of many disease, such as ankylosing spondylitis, lung cancer, rheumatoid arthritis, diabetic nephropathy, sickle cell disease, gastric cancer, and juvenile idiopathic arthritis [15][16][17][18][19][20][21], few studies have been conducted with respect to sepsis [22,23].…”

Section: Discussionmentioning

confidence: 99%

“…Ayar G et al measured thiol-disulphide homeostasis as oxidative stress marker in critically ill children with sepsis [23]. They found that the levels of native thiol, total thiol, and disulphide were significantly decreased in patients with septis shock and sepsis.…”

Section: Discussionmentioning

confidence: 99%

Thiol/disulphide homeostasis in intensive care unit patients with sepsis and septic shock

Yıldız

2020

Turk J Med Sci

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Sepsis, an exaggerated response to infection by the body, is a frequent problem of intensive care units that respond to physiological and pathological biochemical changes [1]. Although the actual incidence is unknown, it is known that sepsis is still an important cause of mortality in intensive care units worldwide [2]. Concomitant factors such as uncontrolled diabetes mellitus, organ transplantation, surgical history, cirrhosis, and congestive heart failure may increase patients' susceptibility to sepsis. It is known that the level of molecules such as reactive species and/or free radicals increase in the pathogenesis of sepsis. Oxidative stress is an undesirable condition with adverse consequences for the body. Oxidative stress state is the disruption of the balance between the level of antioxidant and oxidant molecules [3]. Overproduction of reactive oxidative species disrupts the structure of proteins and lipids. Due to this effect, breaks in DNA structures and oxidation in proteins and lipids of cell membrane occur [4,5]. A breakdown in the mitochondrial electron transmission systems and an increase in the inducible nitric oxide synthesis are the most important changes that occur [3,6,7]. These mechanisms in sepsis cause Background/aim: Sepsis is a condition caused by infection followed by unregulated inflammatory response which may lead to organ dysfunction. The aim of this study is to be the first in the literature and it has been designed to show the thiol/disulphide changes in patients with sepsis and septic shock and their correlation with acute phase reactants. Material and methods: A total of 113 patients (septic shock 53 and sepsis 60) and 60 healthy control subjects have been enrolled in this study from the period February 2018 to 2019. The patients were divided in 2 groups: nonsurvivors (74) and survivors (39). The investigation includes measurements of native thiol, total thiol, dynamic disulphide bond, oxidized thiol ratio, reduced thiol ratio and thiol oxidation reduction ratio, erythrocyte sedimentation rate, C-reactive protein, and procalcitonin. Results: The findings of this study suggest that changes in thiol levels play a role in the pathogenesis of patients with sepsis and septic shock. Conclusions: Thiol/disulphide homeostasis is impaired in patients with sepsis and septic shock. Understanding the role of thiol/ disulphide homeostasis in sepsis and septic shock may provide different therapeutic intervention strategies for patients.

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“…Previous research demonstrated that patients with hospital-acquired AKI had lower levels of unadjusted plasma free thiols as compared to critically ill patients without AKI and healthy subjects (30). In another study, paediatric patients with AKI also had lower levels of unadjusted plasma free thiols as compared to healthy controls (31).…”

Section: Discussionmentioning

confidence: 91%

Plasma thiol redox status as indicator of acute kidney injury

Boekhoud

Koeze

Slikke

et al. 2020

Preprint

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Introduction: Acute kidney injury (AKI) is associated with the abrupt loss of kidney function resulting in increased morbidity and mortality. Oxidative stress plays an important role in the pathophysiology of AKI. Free thiols (R-SH, sulfhydryl groups) are crucial components of the extracellular antioxidant machinery and reliably reflect systemic oxidative stress. Lower levels of thiols represent higher levels of oxidative stress. In this study, we hypothesized that plasma free thiols are associated with AKI upon admission to the intensive care unit (ICU). Methods: In this study, 301 critically ill patients were included for analysis. Plasma samples were taken upon admission. Plasma levels of albumin-adjusted plasma free thiols were determined and correlated with AKI stage upon ICU admission. Results: Albumin-adjusted plasma free thiols were significantly lower in patients with AKI (n=43, median [interquartile range] 7.28 µmol/g [3.52,8.95]) compared to patients without AKI (8.50 µmol/g [5.82, 11.28]; p < 0.05) upon admission to the ICU. Higher age (B = -0.72, p < 0.001), higher levels of neutrophil gelatinase-associated lipocalin (B = -0.002, p < 0.05), creatinine (B = -0.01, p < 0.05) and lower serum albumin (B = 0.47, p < 0.001) were associated with lower free thiol levels. Further, albumin-adjusted free thiol levels were significantly reduced in patients with sepsis (8.30 [5.52-10.64] µmol/g) compared to patients without sepsis (6.95 [3.72-8.92] µmol/g; p < 0.05). Conclusion: Albumin-adjusted plasma free thiols were significantly reduced in patients with AKI and patients with sepsis compared with patients without AKI and sepsis. Together, these data suggest that free thiol levels are mainly reduced in sepsis-associated AKI.

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Effects of Hemodialysis on Thiol-Disulphide Homeostasis in Critically Ill Pediatric Patients with Acute Kidney Injury

Cited by 12 publications

References 34 publications

Acute Kidney Injury is Associated with Lowered Plasma-Free Thiol Levels

Acute Kidney Injury is Associated with Lowered Plasma-Free Thiol Levels

Thiol/disulphide homeostasis in intensive care unit patients with sepsis and septic shock

Plasma thiol redox status as indicator of acute kidney injury

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