Evaluation of Antioxidant, Immunomodulatory, and Cytotoxic Action of Fractions from <i>Eugenia uniflora</i> L. and <i>Eugenia malaccensis</i> L.: Correlation with Polyphenol and Flavanoid Content

Figueirôa, Evellyne de Oliveira; Silva, Luís Cláudio Nascimento da; Melo, Cristiane Moutinho Lagos de; Neves, José Maria Gomes; Silva, Nicácio Henrique da; Pereira, Valéria Rêgo Alves; Correia, Maria Tereza dos Santos

doi:10.1155/2013/125027

Cited by 30 publications

(22 citation statements)

References 27 publications

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“…The ethyl acetate fraction had the highest antioxidant property. This agrees with the deduction made in other studies which revealed that the ethyl acetate fraction of E. uniflora leaf extract contains higher antioxidant potential compared to the butanol and aqueous fractions of the same extract as a result of higher polyphenols content in ethylacetate fraction 23 . Likewise, polyphenolic compounds have been found to possess a wide range of bioactivity potential and have been indicated by the high activity of fruit extract of Eugenia uniflora in DPPH antioxidant assay compared to the activities of fruit extracts of over thirty edible tropical fruits 24 .…”

Section: Phytochemical Screeningsupporting

confidence: 93%

PHYTOCHEMICAL CONSTITUENTS, ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF Eugenia uniflora Linn. LEAF

Olugbuyiro¹,

Banwo²,

Adeyemi³

et al. 2018

RJC

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In this study, the leaf of Eugenia uniflora, an ornamental plant, was evaluated for its phytochemical constituents, antioxidant property and antimicrobial activity. The methanol extract of the leaf was partitioned into hexane, ethyl acetate and aqueous fractions. The antioxidant activities of the crude extract and fractions were performed using 2, 2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method, and IC50 values were calculated. The antimicrobial activity was tested against five bacteria strains (Pseudomonas aeruginosa, Escherichia coli, Micrococcus varians, Klebsiella pneumonia, Staphylococcus aureus) and two fungal strains (Saccharomyces cerevisiae, Aspergillus niger) using agar well diffusion method. Phytochemical screening indicated the presence of phenols, flavonoids, alkaloids, tannins, cardiac glycosides, saponin glycosides, saponins and anthraquinones in the leaf. The ethyl acetate fraction showed highest antioxidant activity (IC50 value of 0.919 mg/ml) compared with reference standard ascorbic acid (IC50 value of 0.859 mg/ml). The plant fractions demonstrated good antibacterial activities. The ethyl acetate fraction showed MIC values of 1.25 mg/mL, against P. aeruginosa and E. coli; 2.50 mg/mL against A. niger and 5.00 mg/mL against M. varians, K. pneumonia and S. cerevisiae while the aqueous fraction had MIC values of 0.625 mg/mL against S. cerevisiae; 1.25 mg/mL against P. aeruginosa, M. varians, K. pneumonia and A. niger and 2.5 mg/mL against Escherichia coli. The hexane fraction had MIC value of 1.25 mg/mL against Staphylococcus aureus. The results indicate that Eugenia uniflora leaf is rich in natural antioxidants and bioactive compounds which may provide health benefits and serve in the management of bacterial and fungal infections.

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Section: Phytochemical Screeningsupporting

confidence: 93%

PHYTOCHEMICAL CONSTITUENTS, ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF Eugenia uniflora Linn. LEAF

Olugbuyiro¹,

Banwo²,

Adeyemi³

et al. 2018

RJC

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“…Some authors have correlated the immunomodulatory effects with the antioxidant status [75] reported that higher antioxidant capacity of E. malaccensis L. and E. uniflora L. helped to reduce the inflammatory response in in vivo. Plant extracts with antioxidant activity could also have immunomodulatory ability [76] .…”

Section: Resultsmentioning

confidence: 99%

Immunomodulatory Effects of Gallic Acid against Cyclophosphamide- and Cisplatin-induced Immunosuppression in Swiss Albino Mice

Sd¹,

Vijayalaxmi²,

Shenoy³

2018

pharmaceutical-sciences

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Shruthi, et al.: Immunomodulatory Effects of Gallic Acid in Swiss Albino MiceGallic acid is a triphenolic acid, widely distributed in fruits, vegetables and plants and is reported to produce antioxidant, antiinflammatory, antifungal, antiviral and antitumor effects. In the present study, immunomoduatory effect of gallic acid was tested against cyclophosphamide and cisplatin; two widely used anticancer agents induced immunosuppression in Swiss albino mice. Cyclophosphamide and cisplatin are known immunosuppressive agents, which elicit variety of immune responses. In recent years much attention is given for the identification of plants or their bioactive compounds as immunomodulators. Three different doses of gallic acid i.e., 100, 200 and 400 mg/kg weight were administered orally for 7 consecutive days. Cyclophosphamide (50 mg/kg) and cisplatin (10 mg/kg) were administered intraperitoneally as single dose. Levamisole 50 mg/kg was used as standard immunomodulatory drug. 0.5 % carboxymethyl cellulose was used as solvent control. Evaluation of immunomodulatory property of gallic acid was done by using haemagglutination antibody titre response and haematological parameters such as white blood cells, red blood cells, platelet counts and haemoglobin levels. Relative weight of thymus an important lymphoid organ was also determined. Augmentation of antibody titre values and haematological end points clearly indicated immunomodulatory effect of gallic acid against cyclophosphamide and cisplatin-induced myelosuppression in Swiss albino mice. Results indicate that, gallic acid could be used as an adjuvant with immunosuppressive drugs to reduce their adverse effects on immune system.

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“…The total flavonoid content of M. floribunda leaves was higher than those reported for other Myrtaceae species, such as Eugenia uniflora (8.43%), Eugenia malaccensis (4.07%), Eugenia brasiliensis (5.41%), Eugenia beaurepaireana (11.46%) and Eugenia umbelliflora (3.28%) (Figueirôa et al 2013, Magina 2008. This result may be explained because the species M. floribunda lives in areas of sandbank, which is a hostile environment for vegetal growth, and also its flavonoids antioxidant propriety protects the vegetal tissue from the extensive luminosity (Zuanazzi and Montanha 2004).…”

Section: Resultsmentioning

confidence: 58%

Antiproliferative activity in tumor cell lines, antioxidant capacity and total phenolic, flavonoid and tannin contents of Myrciaria floribunda

Tietbohl

Oliveira

Esteves

et al. 2017

An. Acad. Bras. Ciênc.

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Myrciaria floribunda (H. West ex Willd.) O. Berg, Myrtaceae, is a native plant species of the Atlantic Rain Forest, from north to south of Brazil. The lyophilized ethyl acetate extract from the leaves of M. floribunda was investigated for its antiproliferative activity in tumor cell lines, antioxidant capacity and its total phenolic, flavonoid and tannin contents. Antiproliferative activity was tested in vitro against seven human cancer cells and against immortalized human skin keratinocytes line (HaCat, no cancer cell). Antioxidant activity was determined using 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and oxygen radical absorbing capacity (ORAC) assays and total phenolic, flavonoid and tannin contents were determined by spectrophotometric techniques. Ethyl acetate extract of M. floribunda exhibited antiproliferative activity against cancer cell lines with total growth inhibition (TGI) between 69.70 and 172.10 µg/mL. For HaCat cell, TGI value was 213.60 µg/mL. M. floribunda showed a strong antioxidant potential: EC 50 of 45.89±0.42 µg/mL and 0.55±0.05 mmol TE/g for DPPH and ORAC, respectively. Total phenolic content was 0.23±0.013g gallic acid equivalents (GAE)/g extract and exhibited 13.10±1.60% of tannins content. The content of flavonoid was 24.08±0.44% expressed as rutin equivalents. These results provide a direction for further researches about the antitumoral potential of M. floribunda.

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Evaluation of Antioxidant, Immunomodulatory, and Cytotoxic Action of Fractions from Eugenia uniflora L. and Eugenia malaccensis L.: Correlation with Polyphenol and Flavanoid Content

Cited by 30 publications

References 27 publications

PHYTOCHEMICAL CONSTITUENTS, ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF Eugenia uniflora Linn. LEAF

PHYTOCHEMICAL CONSTITUENTS, ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF Eugenia uniflora Linn. LEAF

Immunomodulatory Effects of Gallic Acid against Cyclophosphamide- and Cisplatin-induced Immunosuppression in Swiss Albino Mice

Antiproliferative activity in tumor cell lines, antioxidant capacity and total phenolic, flavonoid and tannin contents of Myrciaria floribunda

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