Antiproliferative activity in tumor cell lines, antioxidant capacity and total phenolic, flavonoid and tannin contents of Myrciaria floribunda

Abstract

Myrciaria floribunda (H. West ex Willd.) O. Berg, Myrtaceae, is a native plant species of the Atlantic Rain Forest, from north to south of Brazil. The lyophilized ethyl acetate extract from the leaves of M. floribunda was investigated for its antiproliferative activity in tumor cell lines, antioxidant capacity and its total phenolic, flavonoid and tannin contents. Antiproliferative activity was tested in vitro against seven human cancer cells and against immortalized human skin keratinocytes line (HaCat, no cancer cell). Antioxidant activity was determined using 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and oxygen radical absorbing capacity (ORAC) assays and total phenolic, flavonoid and tannin contents were determined by spectrophotometric techniques. Ethyl acetate extract of M. floribunda exhibited antiproliferative activity against cancer cell lines with total growth inhibition (TGI) between 69.70 and 172.10 µg/mL. For HaCat cell, TGI value was 213.60 µg/mL. M. floribunda showed a strong antioxidant potential: EC 50 of 45.89±0.42 µg/mL and 0.55±0.05 mmol TE/g for DPPH and ORAC, respectively. Total phenolic content was 0.23±0.013g gallic acid equivalents (GAE)/g extract and exhibited 13.10±1.60% of tannins content. The content of flavonoid was 24.08±0.44% expressed as rutin equivalents. These results provide a direction for further researches about the antitumoral potential of M. floribunda.

Antioxidant and the Efficacy of Sophora secundiflora and Methoxyisoflavones in the Immune Function of Pigeons Vaccinated against Paramyxovirus Serotype-1

“…Various studies suggest that phenolic compounds, specifically the flavonoids promoted the secondary immune response and modulate host resistance. 29,[32][33][34] Our current study showed the isolated and identified of three flavonoids 4 -6 for the first time from S. secundiflora. Also, 5-7-dihydroxy-4′-methoxyisoflavone (biochanin A) 1 and 7-hydroxy-4′methoxyisoflavone (formononetin) 2 were isolated and identified.…”

“…Therefore, it is possible to assert that the activities of the EaE are in suitable correlation with these chemical contents, once these classes of compounds are known as potent molecules with immune modulating and radical scavenging activity. 3,33,49 The vaccinated groups showed a maximum protection of 66.6% at the 14 DPV. The protection percentage of the vaccinated and EaE-treated groups of birds at the DPV of 3, 14, 21 and 35 were 53, 87, 100, and 80%, respectively (Table 4).…”

“…44 The activity of APMV vaccinated groups, as a result of the treatment with successive plant extracts (TeE, EaE, and BuE), might be ascribed to the ability of polyphenolics to reduce oxidative stress, improving the membrane integrity of the cells and enhance the innate immune function. 33 Treatment of vaccinated group with EaE extract, containing the 4'-methoxyisoflavones 1 and 2, may prevent oxidative damage by detoxifying ROS/ free radicals. An effective immune response for preventing of many diseases is related with the activities of antibody via the complement activation and antibody-dependent cell-mediated cytotoxicity pathway, macrophage and T-cell.…”

“…Similar relationships have been widely reported in many plants. 3,33 The difference in antioxidant potential of the extracts may be related to variation in the percentage of phytoconstituents extracted in various solvents. HeE sample has exhibited good antioxidant activity, although it contains less polyphenolic content than other samples.…”

Telomerase Inhibitors from Natural Products and Their Anticancer Potential

“…In addition, TA could protect against skin tumor promotion induced by UV radiation in an in vivo study [ 207 ]. Tietbohl et al [ 208 ] have found that TA possesses anti-proliferative properties, which was tested in vitro against seven human cancer cells and immortalized skin keratinocytes. Animal studies have also shown that regular dietary consumption of TA has strongly demonstrated dose-dependent chemopreventive actions against hepatic tumor development and enhances the survival rate [ 209 , 210 , 211 ].…”