Evaluation of Antioxidant and Immunity Function of Tetramethylpyrazine Phosphate Tablets in Vivo

Guo, Ling; Wang, Aihong; Sun, Yan; Xu, Chong-En

doi:10.3390/molecules17055412

Cited by 24 publications

(16 citation statements)

References 46 publications

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“…Consistent with our findings, Guo et al also found that TET could attenuate cardiac function from myocardial injury via increasing the level of GSH and SOD and decreasing MDA. 32 These data indicated that TET could reverse BUP-induced oxidative damage in SH-SY5Y cells. However, when 3-MA was present, the level of total GS, SOD and MDA returned to the level of the BUP group.…”

Section: Discussionmentioning

confidence: 84%

<p>Tetramethylpyrazine attenuated bupivacaine-induced neurotoxicity in SH-SY5Y cells through regulating apoptosis, autophagy and oxidative damage</p>

Wang¹,

Xia²,

Qiao³

et al. 2019

DDDT

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Background: Bupivacaine (BUP) acts as a local anesthetic, which is extensively used for clinical patients but could generate neurotoxicity in neurons. Tetramethylpyrazine (TET) exhibits strong neuron protective effects against neurotoxicity. Hence, we investigate the effect of TET on BUP-induced neurotoxicity in SH-SY5Y cells. Methods: CCK-8 assay was used to detect cell proliferation in SH-SY5Y cells. In addition, Western blotting was used to examine Bax, Bcl-2, active caspase 3, LC3II, Beclin 1 and p-62 protein levels in cells. Moreover, ELISA assay was used to detect the levels of total glutathione (GS), superoxide dismutase (SOD) and malondialdehyde (MDA) in cells. Results: In this study, we found that TET attenuated the neurotoxicity of BUP on SH-SY5Y cells. Meanwhile, TET alleviated BUP-induced apoptosis in SH-SY5Y cell via decreasing the expressions of active caspase-3 and Bax and increasing the expression of Bcl-2. In addition, monodansylcadaverine staining assay and Western blotting results confirmed that TET induced autophagy in SH-SY5Y cells via increasing the LC3II/I and Beclin 1 levels. Furthermore, TET attenuated BUP-induced oxidative damage in SH-SY5Y cells via upregulation of the levels of total GS and SOD and downregulation of the level of MDA. Interesting, the protective effects of TET against BUP-induced neurotoxicity in SH-SY5Y cells were reversed by autophagy inhibitor 3-methyladenine (3MA). Conclusion: These data indicated that TET may play a neuroprotective role via inhibiting apoptosis and inducing autophagy in SH-SY5Y cells. Therefore, TET may be a potential agent for the treatment of human neurotoxicity induced by BUP.

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Section: Discussionmentioning

confidence: 84%

<p>Tetramethylpyrazine attenuated bupivacaine-induced neurotoxicity in SH-SY5Y cells through regulating apoptosis, autophagy and oxidative damage</p>

Wang¹,

Xia²,

Qiao³

et al. 2019

DDDT

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“…Ligustraine is an alkaloid monomer of the plants Chuangxiong which is widely used in a variety of disease, such as cardiovascular diseases [31] . In recent years, various evidences demonstrated that ligustrazine had anti-oxidant and immunity functions [32] . Previously, we had reported that ligustrazine could suppress peritoneal brosis so as to prevent adhesion formation [19] [20] .…”

Section: Discussionmentioning

confidence: 99%

Effect of Ligustrazine Nanoparticles on Th1/Th2 Balance by TLR4/MyD88/NF-κB Pathway in Rats with Postoperative Peritoneal Adhesion

Yang

Lian

Zhang³

et al. 2020

Preprint

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Background Postoperative peritoneal adhesion (PPA) is regarded as fibrous bands connecting both injured abdominal wall and organs or adjacent tissues and associated with T helper (Th)1 and Th2 differentiation. However, the critical role of immunopathogenesis of adhesion formation was precisely unknown. Previously we demonstrated that ligustrazine have positive effects on adhesion formation. And microarray analysis revealed TLR4/MyD88/NF-κB pathway as an important underlying regulatory mechanism in the onset and development of PPA. To further explore the possible mechanism of PPA and enhance the specificity of ligustrazine delivery to injured sites of rodent models, a new agent polylactic acid (PLA) nanoparticles loaded with ligustrazine, that is, ligustrazine nanoparticles (LN) were applied. Methods Twenty-four SD rats were randomly divided into sham, model, LN and sodium hyaluronate (SH) groups. The structure of LN, including entrapment efficiency (EE) and loading capacity (LC), and in vitro drug release were calculated. Adhesions were scored and the masson’s trichrome staining was used to determine the collagen deposition. The expression of TLR4, MyD88 and NF-κB were measured by qPCR, Immunohistochemistry and western blot assay. Moreover, Th1-related cytokines (IFN-γ, IL-12), Th2-related cytokines (IL-4, IL-6) in the cecum tissue and serum were conducted by ELISA. Results LN had good EE, LC and control-release delivery characters with fairly uniform diameter and spherical morphology. It could effectively prevent adhesion formation after surgery. Besides, it could reduce collagen fibers accumulation, downregulate the expression levels of TLR4, MyD88 and NF-κB, and maintain Th1/Th2 balance. Conclusions Ligustrazine nanoparticles had effective effects on Th1/Th2 balance by regualtingTLR4/MyD88/NF-κB pathway in PPA rats. It may be served as a promising therapy on postoperative adhesion formation.

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“…Ligustrazine, also known as tetramethylpyrazine, has the molecular formula C 8 H 12 N 2 and is an identified chemical component isolated from the Chinese herb Rhizoma Chuanxiong. There are a host of clinical applications for ligustrazine, including preventing ischemic heart and cerebrovascular disease, presumably due to its documented effects on important cellular pathways, such as oxidative stress, inflammation, and angiogenesis . As the clinical indications have increased, ligustrazine's repairing effect on all kinds of trauma adhesion have gradually attracted domestic and foreign researchers .…”

Section: Introductionmentioning

confidence: 99%

Effect of intra‐abdominal administration of ligustrazine nanoparticles nano spray on postoperative peritoneal adhesion in rat model

Yan

Yue

Zeng

et al. 2015

J of Obstet and Gynaecol

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Evaluation of Antioxidant and Immunity Function of Tetramethylpyrazine Phosphate Tablets in Vivo

Cited by 24 publications

References 46 publications

<p>Tetramethylpyrazine attenuated bupivacaine-induced neurotoxicity in SH-SY5Y cells through regulating apoptosis, autophagy and oxidative damage</p>

<p>Tetramethylpyrazine attenuated bupivacaine-induced neurotoxicity in SH-SY5Y cells through regulating apoptosis, autophagy and oxidative damage</p>

Effect of Ligustrazine Nanoparticles on Th1/Th2 Balance by TLR4/MyD88/NF-κB Pathway in Rats with Postoperative Peritoneal Adhesion

Effect of intra‐abdominal administration of ligustrazine nanoparticles nano spray on postoperative peritoneal adhesion in rat model

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