Evaluation of antioxidant and antimutagenic activities of aluminum chloride

Saraç, Nurdan; Uğur, Aysel; Karaca, İnci Rana

doi:10.26650/eor.20197852

Cited by 5 publications

(3 citation statements)

References 31 publications

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“… 2019 ; Sarac et al. 2019 ). The present study showed that the isolated compounds 1 – 4 inhibited α-amylase and thus contributed to the α-amylase inhibition activity of the M. latifolia chloroform extract.…”

Section: Discussionmentioning

confidence: 99%

α-Amylase and dipeptidyl peptidase-4 (DPP-4) inhibitory effects of Melicope latifolia bark extracts and identification of bioactive constituents using in vitro and in silico approaches

Quek

Kassim

Lim

et al. 2021

Pharmaceutical Biology

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Context Melicope latifolia (DC.) T. G. Hartley (Rutaceae) was reported to contain various phytochemicals including coumarins, flavonoids, and acetophenones. Objective This study investigates the antidiabetic and antioxidant effects of M. latifolia bark extracts, fractions, and isolated constituents. Materials and methods Melicope latifolia extracts (hexane, chloroform, and methanol), fractions, and isolated constituents with varying concentrations (0.078–10 mg/mL) were subjected to in vitro α-amylase and dipeptidyl peptidase-4 (DPP-4) inhibitory assay. Molecular docking was performed to study the binding mechanism of active compounds towards α-amylase and DPP-4 enzymes. The antioxidant activity of M. latifolia fractions and compounds were determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging and β-carotene bleaching assays. Results Melicope latifolia chloroform extract showed the highest antidiabetic activity (α-amylase IC 50 : 1464.32 μg/mL; DPP-4 IC 50 : 221.58 μg/mL). Fractionation of chloroform extract yielded four major fractions (CF 1 –CF 4 ) whereby CF 3 showed the highest antidiabetic activity (α-amylase IC 50 : 397.68 μg/mL; DPP-4 IC 50 : 37.16 μg/mL) and resulted in β-sitosterol ( 1 ), halfordin ( 2 ), methyl p -coumarate ( 3 ), and protocatechuic acid ( 4 ). Isolation of compounds 2 – 4 from the species and their DPP-4 inhibitory were reported for the first time. Compound 2 showed the highest α-amylase (IC 50 : 197.53 μM) and β-carotene (88.48%) inhibition, and formed the highest number of molecular interactions with critical amino acid residues of α-amylase. The highest DPP-4 inhibition was exhibited by compound 3 (IC 50 : 911.44 μM). Discussion and conclusions The in vitro and in silico analyses indicated the potential of M. latifolia as an alternative source of α-amylase and DPP-4 inhibitors. Further pharmacological studies on the compounds are recommended.

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Section: Discussionmentioning

confidence: 99%

α-Amylase and dipeptidyl peptidase-4 (DPP-4) inhibitory effects of Melicope latifolia bark extracts and identification of bioactive constituents using in vitro and in silico approaches

Quek

Kassim

Lim

et al. 2021

Pharmaceutical Biology

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“…Our results are consistent with those of previously published studies for both strains regarding treatment with Al. Saraç et al [ 59 ] showed no mutagenic effects in the Ames test for the concentrations of AlCl 3 evaluated (0.025, 0.25, and 1.25 mg/mL plate). In another study, Ahn and Jeffery [ 13 ] verified that Al did not cause mutagenic effects at concentrations of 0.3 and 3.0 mg/L but was suggested as toxic to the TA98 strain.…”

Section: Discussionmentioning

confidence: 99%

Acute Toxic and Genotoxic Effects of Aluminum and Manganese Using In Vitro Models

Francisco

Baldivia

Crispim

et al. 2021

Toxics

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The objective of this study was to use the same concentrations of aluminum (Al) and manganese (Mn) detected previously in groundwater above those permitted by Brazilian law and assess their cytotoxic and genotoxic effects in hamster ovary cell lines and their mutagenic effects through the Salmonella microsome assay. Chinese hamster ovary (CHO) and CHO-XRS5 cells were treated with different concentrations of Al and Mn (0.2 to 2.0 mg/L and 0.1 to 3.0 mg/L, respectively). The Ames test was used to analyze the concentrations of Al and Mn ranging from 0.025 to 1.0 mg/L and 0.0125 to 1.5 mg/L, respectively. Both metals showed cytotoxic effects on both cell lines and two bacterial strains (TA98 and TA100). The genotoxic effects of the highest concentrations of Al and Mn in cell lines showed nuclear buds, micronuclei, and DNA damage; however, none of the concentrations showed a positive mutagenic response in the Ames test. This is one of the few studies to demonstrate the cytotoxic effects of Al and Mn through the Ames test. In addition, the metals caused genomic instability in cell lines. Therefore, this study may help hasten the review of established regulatory standards for human consumption of groundwater.

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“…Importantly, it was observed that co-treatments caused the activation of the MAPK/ERK signaling pathway and as a result of this, the progression of the cell cycle was promoted by increasing the phosphorylation of CHK2 in threonine 68, as well as cell migration increased as a result of the high levels of metalloproteases MMP-2 and MMP-9 [ 19 ]. Presumably, the antioxidant activity of AlCl 3 may also have some connection with these findings since it could mitigate ROS induced by chemotherapeutic agents to some degree [ 89 ].…”

Section: Evidence Of Pollutants Affecting the Efficacy Of Chemotherap...mentioning

confidence: 99%

Can Exposure to Environmental Pollutants Be Associated with Less Effective Chemotherapy in Cancer Patients?

Lagunas‐Rangel

Liu

Schiöth

2022

IJERPH

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Since environmental pollutants are ubiquitous and many of them are resistant to degradation, we are exposed to many of them on a daily basis. Notably, these pollutants can have harmful effects on our health and be linked to the development of disease. Epidemiological evidence together with a better understanding of the mechanisms that link toxic substances with the development of diseases, suggest that exposure to some environmental pollutants can lead to an increased risk of developing cancer. Furthermore, several studies have raised the role of low-dose exposure to environmental pollutants in cancer progression. However, little is known about how these compounds influence the treatments given to cancer patients. In this work, we present a series of evidences suggesting that environmental pollutants such as bisphenol A (BPA), benzo[a]pyrene (BaP), persistent organic pollutants (POPs), aluminum chloride (AlCl3), and airborne particulate matter may reduce the efficacy of some common chemotherapeutic drugs used in different types of cancer. We discuss the potential underlying molecular mechanisms that lead to the generation of this chemoresistance, such as apoptosis evasion, DNA damage repair, activation of pro-cancer signaling pathways, drug efflux and action of antioxidant enzymes, among others.

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Evaluation of antioxidant and antimutagenic activities of aluminum chloride

Cited by 5 publications

References 31 publications

α-Amylase and dipeptidyl peptidase-4 (DPP-4) inhibitory effects of Melicope latifolia bark extracts and identification of bioactive constituents using in vitro and in silico approaches

α-Amylase and dipeptidyl peptidase-4 (DPP-4) inhibitory effects of Melicope latifolia bark extracts and identification of bioactive constituents using in vitro and in silico approaches

Acute Toxic and Genotoxic Effects of Aluminum and Manganese Using In Vitro Models

Can Exposure to Environmental Pollutants Be Associated with Less Effective Chemotherapy in Cancer Patients?

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