Acute Toxic and Genotoxic Effects of Aluminum and Manganese Using In Vitro Models

Francisco, Luiza Flávia Veiga; Baldivia, Débora da Silva; Crispim, Bruno do Amaral; Klafke, Syla Maria Farias Ferraz; Castilho, Pamella Fukuda de; Viana, Lucilene Finoto; Santos, Edson Lucas dos; Oliveira, Kelly Mari Pires de; Barufatti, Alexéia

doi:10.3390/toxics9070153

Cited by 13 publications

(4 citation statements)

References 56 publications

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“…According to Kwon et al 70 , chromosome fragments or chromosomes that do not fit into the nucleus of the daughter cell during telophase may be the primary source of MN formation. Previous studies have demonstrated that Mn exposure causes MN development, supporting our findings 71 , 72 . Mn has the potential to be hazardous, as shown by the fact that it can result in chromosomal breakage or modifications to the mitotic spindle during the cell division process.…”

Section: Resultssupporting

confidence: 92%

Effect of Melissa officinalis L. leaf extract on manganese-induced cyto-genotoxicity on Allium cepa L.

Üstündağ,

Macar,

Kalefetoğlu Macar

et al. 2023

Sci Rep

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Although the antioxidant properties of Melissa officinalis extract (Mox) are widely known, little work has focused on its protective capacity against heavy metal stress. The primary objective of this study was to determine the potential of Mox to mitigate manganese (II) chloride (MnCI2)-induced cyto-genotoxicity using the Allium and comet assays. Physiological, genotoxic, biochemical and anatomical parameters as well as the phenolic composition of Mox were examined in Allium cepa (L.). Application of 1000 µM MnCl2 reduced the rooting percentage, root elongation, weight gain, mitotic index and levels of chlorophyll a and chlorophyll b pigments compared to the control group. However, it increased micronuclei formation, chromosomal abnormality frequencies, tail DNA percentage, proline amount, lipid peroxidation level and meristematic damage severity. The activities of superoxide dismutase and catalase also increased. Chromosomal aberrations induced by MnCl2 were fragment, sticky chromosome, vagrant chromosome, unequal distribution of chromatin and bridge. Application of 250 mg/L Mox and 500 mg/L Mox along with MnCl2 significantly alleviated adverse effects dose dependently. The antioxidant activity bestowed by the phenolic compounds in Mox assisted the organism to combat MnCl2 toxicity. Consequently, Mox exerted remarkable protection against MnCl2 toxicity and it needs to be investigated further as a potential therapeutic option.

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Section: Resultssupporting

confidence: 92%

Effect of Melissa officinalis L. leaf extract on manganese-induced cyto-genotoxicity on Allium cepa L.

Üstündağ,

Macar,

Kalefetoğlu Macar

et al. 2023

Sci Rep

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“…These samples have increased concentration of iron, nickel, and chromium compared to the control: in L14D, a certain amount of eluted Al contributes to genotoxicity [ 27 ]; in B7D, Fe (>2.3 mg/L) indicates ferroptosis [ 28 ]. Synergistic effects of metals can also lead to increased genotoxicity of the samples [ 29 ].…”

Section: Resultsmentioning

confidence: 99%

“…Genotoxicity was mainly found in the samples with increased concentration of iron, nickel, and chromium compared to the control. The eluate incubated with all parts for 3 days has nine times more aluminum than the control, which also contributes to genotoxicity [ 27 ]. Synergistic effects of metals can also lead to increased genotoxicity of samples [ 29 , 31 ].…”

Section: Discussionmentioning

confidence: 99%

Toxicity of Metal Ions Released from a Fixed Orthodontic Appliance to Gastrointestinal Tract Cell Lines

Durgo

Orešić

Mlinarić³

et al. 2023

IJMS

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The mechanism of toxicity and cellular response to metal ions present in the environment is still a very current area of research. In this work, which is a continuation of the study of the toxicity of metal ions released by fixed orthodontic appliances, eluates of archwires, brackets, ligatures, and bands are used to test the prooxidant effect, cytotoxicity, and genotoxicity on cell lines of the gastrointestinal tract. Eluates obtained after three immersion periods (3, 7, and 14 days) and with known amounts and types of metal ions were used. Four cell lines—CAL 27 (human tongue), Hep-G2 (liver), AGS (stomach) and CaCo-2 (colon)—were treated with each type of eluate at four concentrations (0.1×, 0.5×, 1.0×, and 2.0×) for 24 h. Most eluates had toxic effects on CAL 27 cells over the entire concentration range regardless of exposure time, while CaCo-2 proved to be the most resistant. In AGS and Hep-G2 cells, all samples tested induced free radical formation, with the highest concentration (2×) causing a decrease in free radicals formed compared to the lowest concentrations. Eluates containing Cr, Mn, and Al showed a slight pro-oxidant effect on DNA (on plasmid φX-174 RF I) and slight genotoxicity (comet assay), but these effects are not so great that the human body could not “resist” them. Statistical analysis of data on chemical composition, cytotoxicity, ROS, genotoxicity, and prooxidative DNA damage shows the influence of metal ions present in some eluates on the toxicity obtained. Fe and Ni are responsible for the production of ROS, while Mn and Cr have a great influence on hydroxyl radicals, which cause single-strand breaks in supercoiled plasmid DNA in addition to the production of ROS. On the other hand, Fe, Cr, Mn, and Al are responsible for the cytotoxic effect of the studied eluates. The obtained results confirm that this type of research is useful and brings us closer to more accurate in vivo conditions.

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“…We used the toxic concentrations (60 μM) of both Fe and Mn, where approximately 40–42% cell death was induced following 24 h incubation with either metal. These concentrations were based on more recent reports ( Francisco et al 2021 ; Zhang N. et al 2022 ) and our previous studies ( Getachew et al 2019 ) where we had observed about 30 percent toxicity with lower concentrations of 20–40 μM of each metal. Hence, we chose a higher concentration of both Fe and Mn to induce significantly more toxicity to allow even subtle protections by our treatments.…”

Section: Methodsmentioning

confidence: 96%

Butyrate protects and synergizes with nicotine against iron- and manganese-induced toxicities in cell culture: Implications for neurodegenerative diseases

Tizabi,

Getachew,

Aschner

2023

Preprint

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Toxic exposures to heavy metals, such as iron (Fe) and manganese (Mn), can result in long-range neurological diseases and are therefore of significant environmental and medical concerns. We have previously reported that damage to neuroblastoma-derived dopaminergic cells (SH-SY5Y) by both Fe and Mn could be prevented by pre-treatment with nicotine. Moreover, butyrate, a short chain fatty acid (SCFA) provided protection against salsolinol, a selective dopaminergic toxin, in the same cell line. Here, we broadened the investigation to determine whether butyrate might also protect against Fe and/or Mn, and whether, if combined with nicotine, an additive or synergistic effect might be observed. Both butyrate and nicotine concentration-dependently blocked Fe and Mn toxicities. The ineffective concentrations of nicotine and butyrate, when combined, provided full protection against both Fe and Mn. Moreover, the effects of nicotine but not butyrate could be blocked by mecamylamine, a non-selective nicotinic antagonist. On the other hand, the effects of butyrate, but not nicotine, could be blocked by beta-hydroxy butyrate, a fatty acid-3 receptor antagonist. These results not only provide further support for neuroprotective effects of both nicotine and butyrate but indicate distinct mechanisms of action for each one. Furthermore, potential utility of the combination of butyrate and nicotine against heavy metal toxicities is suggested.

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Acute Toxic and Genotoxic Effects of Aluminum and Manganese Using In Vitro Models

Cited by 13 publications

References 56 publications

Effect of Melissa officinalis L. leaf extract on manganese-induced cyto-genotoxicity on Allium cepa L.

Effect of Melissa officinalis L. leaf extract on manganese-induced cyto-genotoxicity on Allium cepa L.

Toxicity of Metal Ions Released from a Fixed Orthodontic Appliance to Gastrointestinal Tract Cell Lines

Butyrate protects and synergizes with nicotine against iron- and manganese-induced toxicities in cell culture: Implications for neurodegenerative diseases

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