Evaluation of Antioxidant Activity of Botto-Botto Leaf Fraction (Chromolaena Odorata L.) Using DPPH and ABTS Methods

Tahir, Karlina Amir; Miskad, Upik Anderiani; Djawad, Khairuddin; Sartini, Sartini; Khaerani, Khaerani; Indrisari, Maulita

doi:10.3889/oamjms.2021.5982

Cited by 3 publications

(2 citation statements)

References 15 publications

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“…Very low IC 50 value of essential oil supports the significance of E. odoratum leaves as a promising herbal source of antioxidant compounds, hence can be utilized in nutritional as well as pharmaceutical in industries.Raman et al, 23 reported the DPPH scavenging activity of aqueous and methanol extracts of E. odoratum having IC 50 values 10.5µg/ml and 10.2µg/ml, respectively, that are comparable to the present study. Tahir et al, 24 performed antioxidant activity of the leaf extract by DPPH method, and reported n-butanol fraction have maximum activity with IC 50 value at 33.535µg/ ml. Another study revealed that the DPPH assay of ethyl acetate fraction had the highest antioxidant activity, with IC 50 value of 10.05±0.06 mg/ml.…”

Section: Discussionmentioning

confidence: 99%

GC-MS Characterization of Eupatorium odoratum (L.) Leaves Essential Oil and Evaluation of In vitro Antimicrobial and Antioxidant Activity

Choudhary,

Bithel,

Sharma

et al. 2023

J. Pure Appl. Microbiol.

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The leaves essential oil of Eupatorium odoratum (L.) obtained by the hydro-distillation method was light yellowish in colour, having pungent aromatic fragrance with the yield of 0.010%. Fifteen secondary metabolites were identified via the GC-MS analysis of the essential oil and tau-Cadinol (20.10%) was found to be the major secondary metabolite in the essential oil followed by α Bisabolol (15.33%), cis-muurola-4 (14) 5- diene (10.79%), Isobornyl acetate (6.40%), and Isocaryophyllene (5.39%). The antimicrobial efficacy was investigated via agar well diffusion technique and essential oil was most effective against P. aeruginosa with inhibition zone 22.00±0.57 mm, which was followed by E. coli (19.00±0.57 mm), S. aureus (18.33±0.33 mm), K. pneumoniae (16.50±0.33 mm), and S. pyogenes (11.00±0.33 mm). MIC and MBC values were observed lowest against P. aeruginosa, i.e. 3.12µl/ml and 6.24µl/ml, respectively. Antioxidant activity of essential oil was investigated by DPPH and H2O2 scavenging assay and IC50 value was found very low, i.e. 10.58µl/ml and 11.76µl/ml, respectively, so the essential oil was considered as a potential antioxidant agent. The results validate scientifically the traditional utilization of leaves essential oil of E. odoratum in traditional medicinal system and confirmed that it can be used as modern phytomedicines as an antimicrobial as well as antioxidant agent.

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Section: Discussionmentioning

confidence: 99%

GC-MS Characterization of Eupatorium odoratum (L.) Leaves Essential Oil and Evaluation of In vitro Antimicrobial and Antioxidant Activity

Choudhary,

Bithel,

Sharma

et al. 2023

J. Pure Appl. Microbiol.

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“…This plant is spread all over the world in almost all types of habitats (Aziz, et al, 2020). This plant contains acacetin, chalcones, eupatilin, luteolin, scutellareintetramethyl ether, and sinensetin that have been known to exhibit antibacterial activity, anti-fungi, and antioxidant properties (Atindehou, et al, 2013;Sirinthipaporn & Jiraungkoorskul, 2017;Zahara, 2019;Tahir, et al, 2021). C. odorata is easy to find and has potential source of compounds to be used as a co-chemotherapeutic agent.…”

Section: Introductionmentioning

confidence: 99%

Chromolaena odorata L. Leaf Extract Elevates Cytotoxicity of Doxorubicin on 4T1 Breast Cancer Cells

Putri,

Rahmawati,

Rahman

et al. 2024

Indones J Cancer Chemoprevent

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Chemotherapeutic agents for breast cancer such as doxorubicin can attack normal cells as the side effects. Chromolaena odorata L. and its chemical content, sinensetin, have potential anticancer and antioxidant properties. The objective of this research is to examine the anticancer properties of C. odorata leaves extract and sinensetin on 4T1 triple negative breast cancer (TNBC) cells combined with doxorubicin. The MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5 diphenyltetrazolium bromide) assay on 4T1 cells was used to determine the IC50 and the Combination Index (CI) of the two agents in combination. Washing out the treatment and determining the cells viability after a few days was done to evaluate the persistence of the effects to cancer cells. Chromolaena odorata extract (COE) obtained was proven to contain sinensetin which gave a positive signal on the chromatogram. COE and sinensetin were moderately cytotoxic to 4T1 cells with IC50 value of 53 μg/mL and 58 μM (21.6 μg/mL), respectively. Both compounds were synergist (CI<0.7) to strong synergist (CI<0.3) when combined with doxorubicin (IC50 90 nM = 0.05 μg/mL). COE and sinensetin exhibited moderate and not cytotoxic against Vero cells with IC50 values of 60 μg/mL and 243 μM (90.43 μg/mL), respectively. Both COE and sinensetin showed selectivity index values of >1 (1.13 and 4.19, respectively). Moreover, the cytotoxic effects of COE on 4T1 cells was persisted until 48 h after removing COE from the medium, indicating the tumor-suppression potency of COE. Our findings strengthen the scientific basis of C. odorata leaves extract to be developed as a co-chemotherapeutics agent for doxorubicin on TNBC.Keywords: Chromolaena odorata L., breast cancer cells, doxorubicin, co-chemotherapy, kidney cells.

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Collagen Thickness and Density in BALB/c Mice Exposed to UVB Light after using Siam Weeds Cream (Chromolaena odorata L.)

Tahir

Djawad

Sartini

et al. 2022

RJPT

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Introduction: Ultraviolet B (UVB) radiation can cause damage to the skin's dermis layer, which can lead to collagen fragmentation. Damage to collagen generates wrinkles, which are a sign of aging. Objective: The purpose of this study was to determine how Siam Weeds cream (Chromolaena odorata L.) altered the thickness and density of collagen in the skin of BALB/c mice after UVB exposure. Methods: There were four groups of 24 BALB/c mice altogether: normal control, negative control (Cream Base with UVB), positive control (UVB), and treatment (C. odorata cream and UVB). Just before and after the mice were exposed to UVB at 500 mJ/cm2, the Siam Weeds cream formulation was applied at a concentration of 1%. Mice were slaughtered, and a back skin excisional biopsy was conducted to analyze collagen. Results: the treatment group differed significantly both in thickness and density of collagen against the positive control group and the negative control group (p<0.05) and There was no statistically significant difference between the treatment and normal control groups (p<0.05). Conclusion: A 1% dosage of C. odorata cream can improve the thickness and density of collagen in UVB-exposed mice's skin.

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Evaluation of Antioxidant Activity of Botto-Botto Leaf Fraction (Chromolaena Odorata L.) Using DPPH and ABTS Methods

Cited by 3 publications

References 15 publications

GC-MS Characterization of Eupatorium odoratum (L.) Leaves Essential Oil and Evaluation of In vitro Antimicrobial and Antioxidant Activity

GC-MS Characterization of Eupatorium odoratum (L.) Leaves Essential Oil and Evaluation of In vitro Antimicrobial and Antioxidant Activity

Chromolaena odorata L. Leaf Extract Elevates Cytotoxicity of Doxorubicin on 4T1 Breast Cancer Cells

Collagen Thickness and Density in BALB/c Mice Exposed to UVB Light after using Siam Weeds Cream (Chromolaena odorata L.)

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