Evaluation of antimycobacterial activity of medicinal plants used by Malian traditional medicine practitioners to treat tuberculosis

Abstract

Global Tuberculosis (TB) control is facing major challenges such as occurrence of multidrug-resistant (MDR) and extensively drug-resistant tuberculosis (XDR). The current TB drugs are getting less effective and associated with side effects limiting their use, especially with MDR and XDR infected patients. In Mali, many medicinal plants are used against various diseases including bacterial infections. The study aimed at studying the antimycobacterial activities of 60 extracts from 22 Malian medicinal. The antibacterial activity against Mycobacterium tuberculosis H37Rv was assessed employing micro-broth dilution method. Out of 60 extracts evaluated, eleven from nine different plants were found to be active against H37Rv strain. The minimal inhibitory concentrations (MICs) ranked from 125 μg/mL to 1250 μg/mL. The most active extracts (125 μg/mL) were represented by ethanolic extract of Saba senegalensis and Vitellaria paradoxa leaves, dichloromethane extract of Cola cordifolia leaves, Strychnos spinosa and Ximenia Americana roots. Ethanolic extract of Zizyphus mauritiana, Guiera senegalensis and methanolic extract of Anthocleista djalonensis also prevented the growth of H37Rv at 250 μg/mL. The results suggest that Saba senegalensis, Vitellaria paradoxa, Cola cordifolia, Strychnos spinosa and Ximenia Americana could be potential sources of antimycobacterial molecules

Acute toxicity, hepato-curative activity of extracts of a combination of plants on CCL4-induced hepatotoxicity in rats and antiradical activity

“…The extraction was performed following the method described previously with few adjustments [7]. To prepare the aqueous decoction, we mixed 30 g of powders with 300 mL distilled water, boiled the mixture for 15 minutes, let it cooled down and then filtered through a gaze and cotton.…”

“… Group 1: Neutral controls: rats received olive oil (ip) on day 1 followed by distilled water (10 mL/kg per oral route) for seven (7) consecutive days.  Group 2: Negative controls: rats were intoxicated with CCl4 (ip) on day 1, followed by distilled water (10 mL/kg oral route) for seven (7) consecutive days.…”

“… Group 1: Neutral controls: rats received olive oil (ip) on day 1 followed by distilled water (10 mL/kg per oral route) for seven (7) consecutive days.  Group 2: Negative controls: rats were intoxicated with CCl4 (ip) on day 1, followed by distilled water (10 mL/kg oral route) for seven (7) consecutive days.  Group 3: Positive control: rats were intoxicated with CCl4 (ip) day 1, then treated with silymarin (10 mg/kg per oral route) for seven (7) consecutive days.…”

“… Group 2: Negative controls: rats were intoxicated with CCl4 (ip) on day 1, followed by distilled water (10 mL/kg oral route) for seven (7) consecutive days.  Group 3: Positive control: rats were intoxicated with CCl4 (ip) day 1, then treated with silymarin (10 mg/kg per oral route) for seven (7) consecutive days.  Group 4-6: Rats were intoxicated with CCl4 (ip) day 1, then respectively treated with 400, 200 and 100 mg/kg per oral route of LAD from the mixture.…”

Comparative study of the nutritional potential of a wild fruit (Saba senegalensis) from Côte d'Ivoire with pineapple, papaya and banana

“…The interest of Saba senegalensis in feeding and treating hundreds of diseases in rural Populations is widely recognized (Gning et al, 2013). Indeed, it is used for the treatment of certain diseases such as dysentery, diarrhoea and cough (Koné et al, 2012;Gning et al, 2014;Ballo et al, 2020;Angaman et al, 2001). The fruit consists of a globular shell enclosing very soft and juicy yellow pulp seeds (Kini et al, 2008).…”