Evaluation of antimicrobial properties of four plant extracts against human pathogens

Rawani, Anjali; Pal, Sudin; Chandra, Goutam

doi:10.1016/s2221-1691(11)60127-5

Cited by 29 publications

(22 citation statements)

References 11 publications

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“…This is reflected by the zone of inhibition observed in the inoculated nutrient agar plates and further confirmed by the microdilution broth method. Various plant extracts have been shown to possess anti-microbial activity based on disc diffusion assay (Rawani et al 2011). The variation observed among plant extracts in terms of anti-microbial activity may be attributed to the nature of the phyto-constituents or its geographic origin, particularly the climatic conditions in which the plant is growing (Ncube et al 2008).…”

Section: Discussionmentioning

confidence: 99%

Agrobacterium rhizogenes-mediated transformation of a multi-medicinal herb,Boerhaavia diffusaL.: optimization of the process and anti-microbial activity against bacterial pathogens causing urinary tract infections

Sahu

Jena

Swain

et al. 2013

Frontiers in Life Science

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Hairy root cultures (HRCs) of Boerhaavia diffusa L., an ethno-medicinally important herb, were established via explant co-cultivation with Agrobacterium rhizogenes. Several key factors influencing transformation efficiency were optimized. Rhizoclones showing prolific growth were maintained through successive subcultures in solidified Murashige & Skoog's medium without growth regulators (MS0) under diffused illumination. PCR analysis of the rhizoclones demonstrated positive amplification of rolB and rolC genes of pRi T L -DNA. Reverse transcription-PCR (RT-PCR) and opine assay revealed the presence and expression of the pRi T R -DNA borne ags gene. The methanol and dichloromethane (M:DM) extracts of hairy roots (HRs) and natural roots (NRs) were screened against a wide range of bacterial pathogens causing human urinary tract infections (UTIs). The MIC and MBC values of HRs were markedly lower when compared to NRs against microorganisms causing UTIs. Extracts of HRs were more effective than those of NRs not only against wild type strains but also, and more importantly, against multi-drug resistant strains. Establishment of fast-proliferating renewable HRCs of B. diffusa would provide a direct source of anti-microbial phytocompounds against several pathogenic bacteria, including resistant strains, causing UTIs.

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Section: Discussionmentioning

confidence: 99%

Agrobacterium rhizogenes-mediated transformation of a multi-medicinal herb,Boerhaavia diffusaL.: optimization of the process and anti-microbial activity against bacterial pathogens causing urinary tract infections

Sahu

Jena

Swain

et al. 2013

Frontiers in Life Science

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“…The healthy and disease free plant identified with the aid of keys compiled by Obot and Ayeni [18] were initially rinsed with distilled water and dried on paper towels in the laboratory at (37±1)°C for 24 h [9] for antibacterial activities test and phytochemical analysis.…”

Section: Plant Materialsmentioning

confidence: 99%

“…Use of medicinal plants as a drug is alternative method for the management of pathogenic microbes like bacteria, fungi and viruses is eco-friendly. Different researches conducted worldwide to proof the antimicrobial efficacy of traditional used medicinal plants [7,9,10]. Traditional medicines are well and widely documented in developing countries.…”

Section: Introductionmentioning

confidence: 99%

Phytochemical screening and antibacterial activities of crude extract of Nymphaea lotus (water lily) against fish pathogens

et al. 2016

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Background This study was designed to determine the role of aqueous extract of water lily (Nymphaea lotus) for potential antibacterial activity against some gram-negative fish pathogenic bacteria.Material and methods Aqueous extracts of the dried whole plant Nymphaea lotus was tested by the agar disc diffusion method against four bacteria strains, namely, Escherichia coli, Vibrio anguillarum, Pseudomonas fluorescens, Aeromonas hydrophila and Salmonella typhi. Inhibitory growth values were determined in colony forming unit per gram (cfu/g) for each pathogen while examination of phytochemical constituents of the crude extricate of the plant was also carried out qualitatively.Results Phytochemical screening of the crude aqueous extract revealed the high presence of saponins, alkaloids and carbohydrate, moderate presence of cardiac glycosides, tannins, phenolics, anthroquinones, terpenoid, quinones, catechins and trace of flavonoids.Conclusion The antibacterial analysis of crude aqueous extracts showed moderate varying degrees of resistance against the microbes especially Escherichia coli and Vibrio anguillarum where complete growth inhibition was observed. Hence, this plant aqueous extract can help in the treatment of fish bacterial infections.Journal of Biomedical Sciences. 2015;2(4):38-42.

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“…Plants have been used globally in the treatment of various infectious diseases as a traditional medicine for thousands of years. Currently, a number of pharmaceutical companies are spending a lot of money and time to develop cost-effective plantderived natural drugs [3]. Plants are the rich source of many secondary plant metabolites, such as tannins, terpenoids, alkaloids, flavonoids, phenols, and quinines [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

An in Vitro Investigation of Antimicrobial Efficacy of Euphorbia Hirta and Murraya Koenigii Against Selected Pathogenic Microorganisms

Gupta¹,

Kumar²,

Gupta³

2018

Asian J Pharm Clin Res

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Objective: To investigate the solvent-dependent antimicrobial activity and phytochemical analysis of extracts of Euphorbia hirta (leaves and flowers) and Murraya koenigii (leaves), as well as to evaluate the synergistic activity of these medicinal extracts with suitable antibiotic discs and antibiotics susceptibility of selected pathogenic microorganisms. Methods:The antimicrobial activity of the medicinal extracts was screened through agar well diffusion method and antibiotics susceptibility of selected microorganisms was investigated using disc diffusion method. A combined agar well diffusion and disc diffusion methods were used for the determination of synergistic activities of the extracts with antibiotic discs.Results: Among the different solvents, ethanol had maximum zone of inhibition against the test pathogens. Ethanolic leaf extracts of E. hirta exhibited the highest inhibitory activity against Candida albicans and Staphylococcus aureus with minimum inhibitory concentration value of 12.5 mg/mL and 25.0 mg/mL, respectively. Antimicrobial assay revealed that E. hirta extracts were active against all tested Gram-negative bacteria. However, none of the plant extracts had inhibitory activity against Gram-positive bacterium Propionibacterium acnes. Phytochemical screening for both the extracts from E. hirta revealed the presence of steroid, tannin, terpenoids, carbohydrates, alkaloid, flavonoid, diterpene, and glycoside, whereas M. koenigii extract was rich in saponins, protein, steroid, tannin, carbohydrates, alkaloid, flavonoid, and glycoside. Conclusion:The present study proposes that E. hirta and M. koenigii extracts are excellent sources of natural bioactive compounds that could be used as potent antimicrobial drugs to counter the emerging problem of antibiotic resistance of pathogenic microorganisms.

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Evaluation of antimicrobial properties of four plant extracts against human pathogens

Cited by 29 publications

References 11 publications

Agrobacterium rhizogenes-mediated transformation of a multi-medicinal herb,Boerhaavia diffusaL.: optimization of the process and anti-microbial activity against bacterial pathogens causing urinary tract infections

Agrobacterium rhizogenes-mediated transformation of a multi-medicinal herb,Boerhaavia diffusaL.: optimization of the process and anti-microbial activity against bacterial pathogens causing urinary tract infections

Phytochemical screening and antibacterial activities of crude extract of Nymphaea lotus (water lily) against fish pathogens

An in Vitro Investigation of Antimicrobial Efficacy of Euphorbia Hirta and Murraya Koenigii Against Selected Pathogenic Microorganisms

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