Evaluation of antimicrobial activity of selected plant extracts by rapid XTT colorimetry and bacterial enumeration

Al‐Bakri, Amal G.; Afifi, Fatma U.

doi:10.1016/j.mimet.2006.05.013

Cited by 122 publications

(68 citation statements)

References 22 publications

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“…Al-Bakri and Afifi expressed that some trepenoids, alkaloids and phenolic constitutes of Ruta graveolense are accounted for its antibacterial activity on Bacillus subtilis and Staphylococcus aureus 20 . Chacko et al, demonstrated that in evaluation of three different solvents included hexane, petroleum ether and methanol the best of them in larvicidal activity was petroleum ether solvent for R. graveolens 21 .…”

Section: Resultsmentioning

confidence: 99%

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2018

IJPSR

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Introduction:Klebsiella pneumoniae is one of the most frequent infection organism following orthopedic surgeries and specifically total hip arthroplasty. Recently, attitude toward herbal medicine is being popular both in people and health care systems. Ruta graveolens is a traditional herbal remedy Iranian medicine and some other countries with a wide spectrum of therapeutic utilities. Our purpose was to determine the effects of this medicinal plant on Klebsiella pneumoniae growth. Materials and methods: Standard Klebsiella pneumoniae was used. We assessed the hexane extracts of both leaf and stark of Ruta graveolens on growth of bacterial disc diffusion and serial dilution methods. We compared the results with some standard frequent chemical antibiotics. Results:Our results showed that all doses of leave extract including 200, 250, 300, 350 and 400 micrograms of stark extract had inhibited the growth. In disc diffusion test 500 μg of hexanic extract prevent the growth of bacteria rather than antibiotic discs. We determined minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) by doing their methods. Conclusion: Hexanic extract of Ruta can affect the growth of Klebsiella pneumoniae both for bactericidal and inhibitory effects. It can be a good candidate for more research in vivo to introduce new agent for treating this type of infection.

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Section: Resultsmentioning

confidence: 99%

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2018

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“…Antimicrobial activity of ethanolic extract from leaves and flowers of M. vulgare were evaluated by rapid 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) colorimetry method and potential ethanol extract was further tested by bacterial enumeration methods [105]. M. vulgare showed promising antimicrobial activity against Bacillus subtilis and Staphylococcus aureus.…”

Section: Antimicrobial Activitymentioning

confidence: 99%

Marrubium vulgare L.: A review on phytochemical and pharmacological aspects

Lodhi¹,

Vadnere²,

Sharma³

et al. 2017

J Intercult Ethnopharmacol

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Marrubium vulgare L. (family: Lamiaceae), also known as the white horehound, is widely used as an herbal remedy for chronic coughs and colds. It is used in various disorders related to skin, liver, gastric, heart, and immune system. This review abridges phytochemical, pharmacological studies, and medicinal uses of M. vulgare and provides scientific proof for various ethnobotanical claims to identify gaps, which will give impulsion for novel research on M. vulgare based herbal medicines. This review summarizes selected scientific evidence on phytochemistry and pharmacological properties of M. vulgare over the past 48 years . Works related to M. vulgare was reviewed from various sources such as books, internet source, i.e., Google Search engine, PubMed, and Science Direct, and chemical abstract. The exhaustive literature was studied, and critical analysis was performed according to their phytochemical and pharmacological properties. Phytochemical investigations on different parts of M. vulgare have been reported the presence of flavonoids, steroids, terpenoids, tannins, saponins, and volatile oils (0.05%). The aerial parts contain marrubiin, together with ursolic acid and choline. Pharmacological activities such as antinociceptive, antispasmodic, antihypertensive, antidiabetic, gastroprotective, anti-inflammatory, antimicrobial, anticancer, antioxidant, and antihepatotoxic activity have been reported. M. vulgare has therapeutic potential in the treatment of inflammatory conditions, liver disorders, pain, cardiovascular, gastric, and diabetic conditions. Aerial parts of M. vulgare is a good source of labdane type diterpene especially marrubiin which is present in high concentrations. However, further scientific studies are needed to explore clinical efficacy, toxicity and to explore the therapeutic effect of major secondary metabolites such as diterpenes, phenylpropanoid, and phenylethanoid glycosides of M. vulgare.

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“…In this method relative reduction in formazan production, which was produced by reduction of XTT salt by dehydrogenase enzymes of Electron Transport System (ETS), was measured colorimetrically at 490 nm 15 . Only those extracts, which showed maximum inhibitory effect against different bacteria in comparison to others as demonstrated by agar well diffusion method, were evaluated for this method.…”

Section: Leaves Collection and Processingmentioning

confidence: 99%

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2017

IJPSR

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Inconsequential use of broad-spectrum antibiotics results in the evolution of multiple drug resistant pathogenic gram-negative bacterial strains which is a global health threat and is responsible for high morbidity and mortality rates. This necessitated the search for new effective and safe treatment strategies. One such successful treatment strategy is combination therapy which comprises cow urine distillate and medicinal plants extract. In the present report, this combination therapy was used against five pathogenic gram-negative bacteria-S. typhimurium, K. pneumoniae, E. coli, E. aerogenes and V. cholerae. It was observed that combination therapy had more profound effect than the individual plant extract against all the bacterial strains at the specified level. Combination therapy with T. erecta plant extract was found to be the most effective which improved the antibacterial effect 2.54 times more against E. coli. Other plants extract also improved their antibacterial potential when used with CUD (R.A.I=1.28 to 2.54). INTRODUCTION: Cell wall of gram-negative bacteria is more complex than gram-positive bacteria, due to an extra outer phospholipidic membrane carrying the lipopolysaccharide components, which makes the cell wall less permeable to antimicrobial substances. Evolution of multidrug-resistant gram-negative bacteria due to incongruous use of broad-spectrum antibiotics is responsible for the 30-70% mortality rate due to treatment failure of hospitalized patients.

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Evaluation of antimicrobial activity of selected plant extracts by rapid XTT colorimetry and bacterial enumeration

Cited by 122 publications

References 22 publications

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Marrubium vulgare L.: A review on phytochemical and pharmacological aspects

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