Untitled

Abstract

Inconsequential use of broad-spectrum antibiotics results in the evolution of multiple drug resistant pathogenic gram-negative bacterial strains which is a global health threat and is responsible for high morbidity and mortality rates. This necessitated the search for new effective and safe treatment strategies. One such successful treatment strategy is combination therapy which comprises cow urine distillate and medicinal plants extract. In the present report, this combination therapy was used against five pathogenic gram-negative bacteria-S. typhimurium, K. pneumoniae, E. coli, E. aerogenes and V. cholerae. It was observed that combination therapy had more profound effect than the individual plant extract against all the bacterial strains at the specified level. Combination therapy with T. erecta plant extract was found to be the most effective which improved the antibacterial effect 2.54 times more against E. coli. Other plants extract also improved their antibacterial potential when used with CUD (R.A.I=1.28 to 2.54). INTRODUCTION: Cell wall of gram-negative bacteria is more complex than gram-positive bacteria, due to an extra outer phospholipidic membrane carrying the lipopolysaccharide components, which makes the cell wall less permeable to antimicrobial substances. Evolution of multidrug-resistant gram-negative bacteria due to incongruous use of broad-spectrum antibiotics is responsible for the 30-70% mortality rate due to treatment failure of hospitalized patients.