Evaluation of antiinflammatory activity of the total flavonoids of Laggera pterodonta on acute and chronic inflammation models

Abstract

The antiinflammatory effect of the total flavonoids of Laggera pterodonta (TFLP) was evaluated with various in vivo models of both acute and chronic inflammation. In the acute inflammation tests, TFLP significantly inhibited xylene-induced mouse ear oedema, carrageenan-induced rat paw oedema and acetic acid-induced mouse vascular permeability. In the carrageenan-induced rat pleurisy model, TFLP efficiently suppressed inflammatory exudate and leukocyte migration, reduced the serum levels of lysozyme (LZM) and malondialdehyde (MDA), increased the activity of serum superoxide dismutase (SOD), and also decreased the contents of total protein, nitric oxide (NO) and prostaglandin E2 (PGE2) in the pleural exudates. No marked effect of TFLP on the activity of serum glutathione peroxidase (GSH-PX) was observed. In the chronic inflammation experiment, TFLP inhibited cotton pellet-induced rat granuloma. The antiinflammatory mechanisms of TFLP are probably associated with the inhibition of prostaglandin formation, influence on the antioxidant systems and the suppression of LZM release. The acute toxicity study revealed that TFLP was nontoxic in mice up to an oral dose of 7.5 g/kg body weight.

In vivo effects of N/OFQ(1–13)NH2 and its structural analogue [ORN9]N/OFQ(1–13)NH2 on carrageenan-induced inflammation: rat-paw oedema and antioxidant status

“…Similar results were reported by others authors [40,55]. Four hours after CG application we also found changes in endogenous cell antioxidants: GSH level and SOD activity decreased, and GSH-Px activity increased in tissues from right, but not from left paws in accordance with published data [40,51,53]. Additionally, we found a strong increase of Glu-6-P-DH activity.…”

“…Moreover, CG-induced inflammation is found to be useful model for testing the antioxidant efficacy of different substances, as celecoxib, local anesthetics, arrhythmic agents, flavonoids and many other including anti-inflammatory drugs [40,41,43,[45][46][47][48][49][50][51][52][53][54]. For example, the anti-inflammatory effects of n-acetylcysteine may include: an inhibition of neutrophil infiltration and prevention of the activation of NADPH oxidase, responsible for O 2 -production; inhibition of the activation of NF-kB and prevention of the expression of iNOS, responsible for NO .…”

Evaluation of anti-inflammatory and antioxidant activities of Peltigera rufescens lichen species in acute and chronic inflammation models

“…In addition to neutrophil-derived free radicals, production of nitric oxide (NO) plays an important role in the development of the edema caused by various factors, including the effects induced by prostaglandins and cytokines [2,[25][26][27][28][29]. Nitric oxide synthesized by nitric oxide synthase in activated inflammatory cells acts as a secondary mediator of some actions of pro-inflammatory cytokines [2,25,[27][28][29][30].…”

Development of HPLC fingerprint for the quality control ofEuonymus fortuneiand distinguishing it from related species

“…Thus, these compounds are usually chosen as marker compounds to access the quality of herbal medicine in the literature [13][14][15]. The total flavonoid content is frequently used as an important reference to be determined in raw material or aqueous extracts [16][17][18][19].…”